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The map label for this gene is mesJ [H]

Identifier: 124021791

GI number: 124021791

Start: 75210

End: 76241

Strand: Reverse

Name: mesJ [H]

Synonym: P9303_00761

Alternate gene names: 124021791

Gene position: 76241-75210 (Counterclockwise)

Preceding gene: 124021797

Following gene: 124021790

Centisome position: 2.84

GC content: 55.04

Gene sequence:

>1032_bases
ATGGCCTCATCCAGCTCAGCGCAGCTGCCCTGGAGCGCCTGGCACGCGCGCCTCCATCACAAACTGCTAGCGAATCCAGA
TCTACTGCCAGCAGAAGCCTCACTCTTGATCGCCGTGTCCGGTGGTCAAGATTCAATGGCCCTGCTCGGCCTCTTGATCG
ATCTCCAACGAAAACATGGCTGGTCTTTAGAGGTGTGGCATGGCGACCACAACTGGCATCAACAATCAGCAACGATCGCA
ACTGAACTCAAGGATTGGTGTGAAGCACACAACTTGAGCTTCTGGTCTGATCAAGCCAAACCTGGACAAACCAACAACGA
AGCCACAGCGCGACATTGGCGATATGAACAACTGACGCTTCACACAGAACGTTTGTCATCCAACAACCCAAACCATCCAT
GCAGGTATGTGCTCACAGGCCACACCAGTTCTGATCGAGCTGAAACCTTGCTACTCAACCTTGCCCGTGGTGCGGACCTG
GCGGGACTGAGCAGCATGCGCCAATGCAGGCCCATCAGCAAAGACAATCCTCGCAAAAACGTGCAGCTCATACGCCCTTT
GCTGGGGTTCTCGCGCGAGGACACCGCCCAGATTTGCAGCGAGCTGTCCTTACCTATCTGGCTTGATCCTGCCAATTCCA
ATCCAGACTTCAGCCGCAACCGAGTCCGCCAAGAAGTGCTCCCAGTACTGGAATCTCTCCATCCGGGCTGCAGCCTGCGC
ATAGCCGCTCTCGCCGAAAGGCTCAACCATCACCATGCGGACCAGCAAGCCATCGCCATGCTTGCTCTAAACAACCTCAG
CAAGCAAAACGGCCTCTGCCGACAGGACCTAGCCCAACTACCGATCACAGCAAGAACAACTCTTTTGGCCTATTGGCTAA
AGCGATCAGGAGCACCAAGCCTGCCAGCAGTCCAACTTGAACAGATCAGCCAAAGCATTGGCCCAGGAAAACCACCTGGA
TCTCTACACCTCGCGCAGGGCTGGAAAGTCCAATGGCAGCGAAACTCGGTACAACTAGAACATCACGACTGA

Upstream 100 bases:

>100_bases
TGAGCGAGGCGGGTCATTCAATCAACGATCTGTAGATCCATATTCCCATTAGCCAGGCTCCCGCACGGCAGACTGGCCTC
AGCTGAGTGACCAGTGCGCT

Downstream 100 bases:

>100_bases
CTCCGGTGGACGGCGACTTCCAACAGCAATACCTGGCCGCAGAGAAGGCCTATGGGGCAGGAAATTACGAAGCAGCGCAA
TCAATCTGTGAACGCCTGCT

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MASSSSAQLPWSAWHARLHHKLLANPDLLPAEASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHQQSATIA
TELKDWCEAHNLSFWSDQAKPGQTNNEATARHWRYEQLTLHTERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGADL
AGLSSMRQCRPISKDNPRKNVQLIRPLLGFSREDTAQICSELSLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
IAALAERLNHHHADQQAIAMLALNNLSKQNGLCRQDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIGPGKPPG
SLHLAQGWKVQWQRNSVQLEHHD

Sequences:

>Translated_343_residues
MASSSSAQLPWSAWHARLHHKLLANPDLLPAEASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHQQSATIA
TELKDWCEAHNLSFWSDQAKPGQTNNEATARHWRYEQLTLHTERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGADL
AGLSSMRQCRPISKDNPRKNVQLIRPLLGFSREDTAQICSELSLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
IAALAERLNHHHADQQAIAMLALNNLSKQNGLCRQDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIGPGKPPG
SLHLAQGWKVQWQRNSVQLEHHD
>Mature_342_residues
ASSSSAQLPWSAWHARLHHKLLANPDLLPAEASLLIAVSGGQDSMALLGLLIDLQRKHGWSLEVWHGDHNWHQQSATIAT
ELKDWCEAHNLSFWSDQAKPGQTNNEATARHWRYEQLTLHTERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGADLA
GLSSMRQCRPISKDNPRKNVQLIRPLLGFSREDTAQICSELSLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLRI
AALAERLNHHHADQQAIAMLALNNLSKQNGLCRQDLAQLPITARTTLLAYWLKRSGAPSLPAVQLEQISQSIGPGKPPGS
LHLAQGWKVQWQRNSVQLEHHD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=204, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 38308; Mature: 38177

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSSAQLPWSAWHARLHHKLLANPDLLPAEASLLIAVSGGQDSMALLGLLIDLQRKHG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
WSLEVWHGDHNWHQQSATIATELKDWCEAHNLSFWSDQAKPGQTNNEATARHWRYEQLTL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHEE
HTERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGADLAGLSSMRQCRPISKDNPRKN
EHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHH
VQLIRPLLGFSREDTAQICSELSLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
HHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
IAALAERLNHHHADQQAIAMLALNNLSKQNGLCRQDLAQLPITARTTLLAYWLKRSGAPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LPAVQLEQISQSIGPGKPPGSLHLAQGWKVQWQRNSVQLEHHD
CCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCEEEECCC
>Mature Secondary Structure 
ASSSSAQLPWSAWHARLHHKLLANPDLLPAEASLLIAVSGGQDSMALLGLLIDLQRKHG
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
WSLEVWHGDHNWHQQSATIATELKDWCEAHNLSFWSDQAKPGQTNNEATARHWRYEQLTL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHEE
HTERLSSNNPNHPCRYVLTGHTSSDRAETLLLNLARGADLAGLSSMRQCRPISKDNPRKN
EHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHH
VQLIRPLLGFSREDTAQICSELSLPIWLDPANSNPDFSRNRVRQEVLPVLESLHPGCSLR
HHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
IAALAERLNHHHADQQAIAMLALNNLSKQNGLCRQDLAQLPITARTTLLAYWLKRSGAPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LPAVQLEQISQSIGPGKPPGSLHLAQGWKVQWQRNSVQLEHHD
CCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]