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The map label for this gene is gap2 [H]

Identifier: 124021742

GI number: 124021742

Start: 30825

End: 31955

Strand: Direct

Name: gap2 [H]

Synonym: P9303_00271

Alternate gene names: 124021742

Gene position: 30825-31955 (Clockwise)

Preceding gene: 124021738

Following gene: 124021745

Centisome position: 1.15

GC content: 49.78

Gene sequence:

>1131_bases
TTGACCTTGTGCCAGGTATGGGCCGCCTCCTCATCCGGAACAGCGGAAAGTGCAACCTATTTCTGTATGATCGGCGGCCA
CTTCCCTTGCGGTTTCCCCGTTTTTGTTATGACTCTGCGCGTTGCGATCAATGGATTCGGCCGAATCGGTCGCAACTTCA
TGCGTTGTTGGCTAAGCCGTGGCGTGAATACTGGGATTGAAGTGGTTGGCGTCAACGTCACTTCTGACCCTAAGACCAAT
GCTCATCTGCTCAAGTACGATTCAATTCTTGGACAGATCAAGGATGCGGAGATCGATTATACCGATGACACTTTTGTTAT
CAATGGCAAACCAATTAAGTGTTTTTCAGATCGCAACCCCTTGAATCTTCCTTGGAAGGAGTGGGGTATCGATCTTGTGA
TCGAGTCCACCGGGGTTTTCAATACTGATGTCGGGGCTAGTAAACACATCGAGGCTGGTGCGAAGAAAGTGATCCTCACA
GCTCCAGGTAAAGGAGATGGTGTGGGCACATATGTCGTAGGCGTCAATGCTGATCAGTACCGTCACGGTGATTTCAATGT
TCTCAGTAATGCCAGCTGCACCACCAATTGCTTGGCTCCAATTGTCAAAGTTTTAGATCAGAATTTCGGAATGGAATGGG
GTTTGATGACCACCATTCACAGCTATACCGGTGACCAGAGAATTCTTGACAACAGCCACCGTGATCTACGCCGCGCTCGA
GCAGCAGCGTTGAATATGGTTCCAACCACTACCGGTGCTGCACAGGCTGTTGCGTTGGTTTATCCCGAAGTTAAAGGCAA
GCTCACCGGTTTCGCGATGCGGGTTCCGACTCCAAATGTGTCAGCTGTTGACCTGACTTTCGGAACTTCAAAAGGCCCAA
GCGTTGATGATGTGAAAGCCGTGATGAAGAGTGCTTCTGAGAACGGGATGAAGGGAATCATCAAGTACAGCGATTTGCCT
CTCGTCTCGACCGACTACGCCGGAACGAACGAGTCGTCCATCTTTGATGCTGATCTCACTTATGCCATGGGTGATAAGGC
CGTGAAAATTCTGGCTTGGTATGACAACGAGTGGGGTTATAGCCAGAGGGTTGTCGATCTCGCTGAGATTGTGGCCAAGA
ATTGGAAGTGA

Upstream 100 bases:

>100_bases
TGAGCGTGCTGGTCAATGGGCAAAACCATAGGCTCAAAAGATAAGCCAGTCTTGGGCTGAAAGCCCGTGATTCTTATGTT
TTGGAGAGGTCCAGCCGGGG

Downstream 100 bases:

>100_bases
TCTTTCAGAGGATTACTTTTTTCTGAGCTTCTAGCTTCTTTTTGAGTTACAAACCCCTCCTCTTAGCGAGGGGTTTTTGT
CTTTCTTTATTGGAAATGTT

Product: glyceraldehyde 3-phosphate dehydrogenase(NADP+)(phosphorylating)

Products: NA

Alternate protein names: GAPDH 2; GAP-2; NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTLCQVWAASSSGTAESATYFCMIGGHFPCGFPVFVMTLRVAINGFGRIGRNFMRCWLSRGVNTGIEVVGVNVTSDPKTN
AHLLKYDSILGQIKDAEIDYTDDTFVINGKPIKCFSDRNPLNLPWKEWGIDLVIESTGVFNTDVGASKHIEAGAKKVILT
APGKGDGVGTYVVGVNADQYRHGDFNVLSNASCTTNCLAPIVKVLDQNFGMEWGLMTTIHSYTGDQRILDNSHRDLRRAR
AAALNMVPTTTGAAQAVALVYPEVKGKLTGFAMRVPTPNVSAVDLTFGTSKGPSVDDVKAVMKSASENGMKGIIKYSDLP
LVSTDYAGTNESSIFDADLTYAMGDKAVKILAWYDNEWGYSQRVVDLAEIVAKNWK

Sequences:

>Translated_376_residues
MTLCQVWAASSSGTAESATYFCMIGGHFPCGFPVFVMTLRVAINGFGRIGRNFMRCWLSRGVNTGIEVVGVNVTSDPKTN
AHLLKYDSILGQIKDAEIDYTDDTFVINGKPIKCFSDRNPLNLPWKEWGIDLVIESTGVFNTDVGASKHIEAGAKKVILT
APGKGDGVGTYVVGVNADQYRHGDFNVLSNASCTTNCLAPIVKVLDQNFGMEWGLMTTIHSYTGDQRILDNSHRDLRRAR
AAALNMVPTTTGAAQAVALVYPEVKGKLTGFAMRVPTPNVSAVDLTFGTSKGPSVDDVKAVMKSASENGMKGIIKYSDLP
LVSTDYAGTNESSIFDADLTYAMGDKAVKILAWYDNEWGYSQRVVDLAEIVAKNWK
>Mature_375_residues
TLCQVWAASSSGTAESATYFCMIGGHFPCGFPVFVMTLRVAINGFGRIGRNFMRCWLSRGVNTGIEVVGVNVTSDPKTNA
HLLKYDSILGQIKDAEIDYTDDTFVINGKPIKCFSDRNPLNLPWKEWGIDLVIESTGVFNTDVGASKHIEAGAKKVILTA
PGKGDGVGTYVVGVNADQYRHGDFNVLSNASCTTNCLAPIVKVLDQNFGMEWGLMTTIHSYTGDQRILDNSHRDLRRARA
AALNMVPTTTGAAQAVALVYPEVKGKLTGFAMRVPTPNVSAVDLTFGTSKGPSVDDVKAVMKSASENGMKGIIKYSDLPL
VSTDYAGTNESSIFDADLTYAMGDKAVKILAWYDNEWGYSQRVVDLAEIVAKNWK

Specific function: Works with both NADP and NAD [H]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=45.3172205438066, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI7669492, Length=334, Percent_Identity=44.311377245509, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1788079, Length=338, Percent_Identity=48.2248520710059, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1789295, Length=330, Percent_Identity=41.2121212121212, Blast_Score=278, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI17534677, Length=342, Percent_Identity=46.4912280701754, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17534679, Length=342, Percent_Identity=46.1988304093567, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32566163, Length=342, Percent_Identity=45.3216374269006, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17568413, Length=342, Percent_Identity=45.3216374269006, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321631, Length=339, Percent_Identity=48.9675516224189, Blast_Score=314, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6322468, Length=340, Percent_Identity=48.8235294117647, Blast_Score=313, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6322409, Length=340, Percent_Identity=48.5294117647059, Blast_Score=305, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17933600, Length=333, Percent_Identity=46.8468468468468, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18110149, Length=333, Percent_Identity=46.8468468468468, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI85725000, Length=333, Percent_Identity=46.8468468468468, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI22023983, Length=333, Percent_Identity=46.8468468468468, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI19922412, Length=329, Percent_Identity=44.3768996960486, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.59 [H]

Molecular weight: Translated: 40753; Mature: 40622

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLCQVWAASSSGTAESATYFCMIGGHFPCGFPVFVMTLRVAINGFGRIGRNFMRCWLSR
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVNTGIEVVGVNVTSDPKTNAHLLKYDSILGQIKDAEIDYTDDTFVINGKPIKCFSDRNP
CCCCCEEEEEEEECCCCCCCCEEEEEHHHHCCCCCCEECCCCCEEEECCCEEEEECCCCC
LNLPWKEWGIDLVIESTGVFNTDVGASKHIEAGAKKVILTAPGKGDGVGTYVVGVNADQY
CCCCHHHCCEEEEEECCCCEECCCCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCE
RHGDFNVLSNASCTTNCLAPIVKVLDQNFGMEWGLMTTIHSYTGDQRILDNSHRDLRRAR
ECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCHHHHHHHH
AAALNMVPTTTGAAQAVALVYPEVKGKLTGFAMRVPTPNVSAVDLTFGTSKGPSVDDVKA
HHHHCCCCCCCCCHHEEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHH
VMKSASENGMKGIIKYSDLPLVSTDYAGTNESSIFDADLTYAMGDKAVKILAWYDNEWGY
HHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCEEECCCCEECCCCEEEEEEEECCCCCC
SQRVVDLAEIVAKNWK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLCQVWAASSSGTAESATYFCMIGGHFPCGFPVFVMTLRVAINGFGRIGRNFMRCWLSR
CEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVNTGIEVVGVNVTSDPKTNAHLLKYDSILGQIKDAEIDYTDDTFVINGKPIKCFSDRNP
CCCCCEEEEEEEECCCCCCCCEEEEEHHHHCCCCCCEECCCCCEEEECCCEEEEECCCCC
LNLPWKEWGIDLVIESTGVFNTDVGASKHIEAGAKKVILTAPGKGDGVGTYVVGVNADQY
CCCCHHHCCEEEEEECCCCEECCCCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCE
RHGDFNVLSNASCTTNCLAPIVKVLDQNFGMEWGLMTTIHSYTGDQRILDNSHRDLRRAR
ECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCHHHHHHHH
AAALNMVPTTTGAAQAVALVYPEVKGKLTGFAMRVPTPNVSAVDLTFGTSKGPSVDDVKA
HHHHCCCCCCCCCHHEEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHH
VMKSASENGMKGIIKYSDLPLVSTDYAGTNESSIFDADLTYAMGDKAVKILAWYDNEWGY
HHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCEEECCCCEECCCCEEEEEEEECCCCCC
SQRVVDLAEIVAKNWK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9226260; 8905231 [H]