Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 124021733

GI number: 124021733

Start: 21983

End: 23059

Strand: Reverse

Name: pilT [H]

Synonym: P9303_00181

Alternate gene names: 124021733

Gene position: 23059-21983 (Counterclockwise)

Preceding gene: 124021734

Following gene: 124021732

Centisome position: 0.86

GC content: 51.25

Gene sequence:

>1077_bases
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAGCC
CCCGTATGGACGCTTCAGCGGTGAATTAAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAGAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTTGGCAGCACTATCCCGAG
TATTGAAAGTCTCAACCTGCCATCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACCCTTGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACATATCCTCACGATC
GAAGATCCAATCGAATTCGTATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGCGAAGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACCGGTCACCTCGTATTCGGCACCTTGCACACCAACTCGGCGGCACAAACGGTCGAT
CGCATGGTTGATGTTTTCCCCCCAAACCAGCAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
GCAAACCCTCTGCCGTCGCCTCAATCCACAACCAGAACAATTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTGACTAAACAGGGTGAAATTAGCCGTTCAGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Upstream 100 bases:

>100_bases
GCGCTTAGCACTCTCACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGGCTGGCTGGAATGTCCCTACTGTCTAACCGC
TCGCCAATGAAGGACCCGTC

Downstream 100 bases:

>100_bases
GAGACAGGCCTAAGCGATGACGTCCTTCGTCGCCACCTACGAATCAGCAAGTGGTCAAACACGCACAATGACCATCAAAG
CGGCCGATCTAACAACCGCA

Product: PilT1-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Sequences:

>Translated_358_residues
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG
>Mature_358_residues
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=332, Percent_Identity=43.9759036144578, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI1789723, Length=310, Percent_Identity=35.4838709677419, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786296, Length=157, Percent_Identity=45.859872611465, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39380; Mature: 39380

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
QRKTLEQIWELDCAYGLKGVARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIET
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEC
SKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTIEDPIEFVYKSDLSLVHQRQL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
NEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLI
HHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
REGKTAQLYSQIQTGGELGMQTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
QRKTLEQIWELDCAYGLKGVARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIET
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEC
SKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTIEDPIEFVYKSDLSLVHQRQL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
NEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLI
HHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
REGKTAQLYSQIQTGGELGMQTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]