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The map label for this gene is tapB [H]

Identifier: 124021734

GI number: 124021734

Start: 23070

End: 24986

Strand: Reverse

Name: tapB [H]

Synonym: P9303_00191

Alternate gene names: 124021734

Gene position: 24986-23070 (Counterclockwise)

Preceding gene: 124021739

Following gene: 124021733

Centisome position: 0.93

GC content: 53.1

Gene sequence:

>1917_bases
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAGTAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTCTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAGTGTGCTTAGCCAAGAAGAACTTGTTGTCGGAGTGGAGCTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCCTGCCAATCAATATGCACAACCAACATTTGGTTGTAGCAATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTGATCTCATTGCTGCAGTCGCAAGGCTTCAGCACAGAATTACGTCTCGCGCTGG
CATCCGACATCAGCCAGCTGCTTGCACCAATGAGATCTGAGCAGCACGGTGAATCTGCATCCAAGTCAAAAGCAACAAAG
CCACTTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGACTTGCTAGTTCCGTAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGCTCACCAGTCATCAACCTGGTGGACC
GCATACTGCTAGAGGCACTGCAAACAGAAGCCAGCGACGTACACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTTTTGCAGCGCTATATCGAACCCCTCCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGCATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTCCTCGACAGCAGTGCTCCACAACTC
GGGCTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGATCAGGCAAGTCAACAACCCTTTACTCCCTACTGGCAGAACGCAACGAGCCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACCCAATGTCAAGTGAACAGGGAAAAAGGCTTCGAT
TTCAGTACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTACTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTGCTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAAATGGGCGTGGAACCATTCATGGTGAGCGCCTCGTTGATTGGCATCGTCTCTCAAAGACTTCTACGA
AGAGTGTGCCGTTCTTGTCGTAAGTCCTATCACCCAACTGAAAAAGAACTGGGGCGATTCGGATTGATGGCCCATACAGA
AACTGGCGTGACCTTTTTCAAGGCCCACCATCACGGTCAAGAAAAACAGCCGTGCCCCAACTGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAATGAAGAGCTCGCTACAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTCGAGGCTGGTATGAAAACTCTGTTGGGCTACAGCCTTGACCTGGTGCGAGAAGGCCACAC
CACCCTTGAGGAAGTGGGCCGAATGATCCTCACCGATTCCGGATTGGAATCAGAGCGCCGCGCCAGAGCGCTTAGCACTC
TCACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGGCTGGCTGGAATGTCCCTACTGTCTAACCGCTCGCCAATGA

Upstream 100 bases:

>100_bases
ACCCTCGTCGGAATTCATCGATGCCACTGATTCCAGAGGCACGGAAGCCTCTAAACCCTCAGCAGCAGGATCGTGAGCTG
GGGTAGGGGCGTCGCCACTC

Downstream 100 bases:

>100_bases
AGGACCCGTCATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTA
CCGGCCAGCCCCCGTATGGA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVSVLSQEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLLQSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATK
PLAQTPTSLLAGFSAEGVLEEDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ

Sequences:

>Translated_638_residues
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVSVLSQEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLLQSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATK
PLAQTPTSLLAGFSAEGVLEEDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ
>Mature_638_residues
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVSVLSQEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLLQSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATK
PLAQTPTSLLAGFSAEGVLEEDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=460, Percent_Identity=43.695652173913, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=38.7179487179487, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI87082188, Length=199, Percent_Identity=40.7035175879397, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 70295; Mature: 70295

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVS
CCEEEECHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VLSQEELVVGVELMANHTTLDISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLL
HCCCCHHEEEEEEHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATKPLAQTPTSLLAGFSAEGVLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCHHHHHCCCCCCCCC
EDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHHEEEEHHHC
PSRYGEKICLRLLDSSAPQLGLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTL
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGE
HHHHHHCCCCCCEEEECCCCHHHCCCCCEEEEECHHCCCCHHHHHHHHHHCCCCEEEEEC
TRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
CCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVY
HHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
EVLRMNEELATAVAKGATTDLVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDS
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCC
GLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVS
CCEEEECHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VLSQEELVVGVELMANHTTLDISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISLL
HCCCCHHEEEEEEHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QSQGFSTELRLALASDISQLLAPMRSEQHGESASKSKATKPLAQTPTSLLAGFSAEGVLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCHHHHHCCCCCCCCC
EDPEEQARLASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHHEEEEHHHC
PSRYGEKICLRLLDSSAPQLGLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTL
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGE
HHHHHHCCCCCCEEEECCCCHHHCCCCCEEEEECHHCCCCHHHHHHHHHHCCCCEEEEEC
TRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
CCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVY
HHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
EVLRMNEELATAVAKGATTDLVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDS
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCC
GLESERRARALSTLTCNVCGAGLQDGWLECPYCLTARQ
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]