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The map label for this gene is speB [C]

Identifier: 124026864

GI number: 124026864

Start: 1815834

End: 1816733

Strand: Direct

Name: speB [C]

Synonym: NATL1_21591

Alternate gene names: 124026864

Gene position: 1815834-1816733 (Clockwise)

Preceding gene: 124026863

Following gene: 124026866

Centisome position: 97.38

GC content: 33.0

Gene sequence:

>900_bases
ATGACCAAATCCAACCTCAAAGATCTATCACTATTTAATAACGATGGTGGAATATTTTTGGGCGCCCAAAGAGGCATCGA
TCAATGCAGAGTGTCACTTTTAGGAGTTCCTTATGATGGAACTTGTTGCTTTAGACCTGGTGCAAGATTTGGACCTTCTG
CTGTTAGGGAAGATAGTTATGGAATTGAAACATATTGTCCGCAATTAAATTTAGATTTAGAAGATATAAAATTTGCTGAT
ATAGGTTCATTAGATGTTCCCCTTGGAGATGCAAAATTAACACTTGATTATATAAAAGATGCTACAAATATTTTACTTAA
AAATAATTTAAAGCCTCTTATTATTGGTGGTGAGCATTCAATAACAAGTGGGATTATTAAATCAATAATTACTAAGTATC
CTGACTTGATTATGCTTCAATTAGATGCTCATGCAGACCTTAGAGATGAATGGTTAGGTAGTAAATTTAGTCATGCATGT
ACTATGAAAAGATGTTTAGAAATACTACCTAGCAAAAAGATTTTTCAAATAGGAATAAGAAGTGGAACAAAATCAGAATT
TCTTGAAATGAATAATAGTAAAAGGTTAATTCAACATACTTTAGGGGAGAATGCAAAATCTTTAGAAGAAGCTCTAAAAA
GTTTTAAAGGTAGACCAATCTATTTAACTTTTGATTTAGATTGGTTTGATCCCTCTGTAATGCCAGGAACAGGCACTCCT
GAGCCCGGAGGCTATTTTTGGGGAGATTTTGCAGCAATAATAAATGTGATTAAGTCTCATAATTTAATTGGTGCTGATGT
AGTCGAATTATCTCCTAAATTAGATAACACTGGTATTAGTAGCATCCTTGCTGCAAAAGTTGTACGTTCATTAATTCTGT
TGTTGGACAAATCATCCTAA

Upstream 100 bases:

>100_bases
TAAAAGAGATCTCCGAAAATTGTCAGGTTTGGAGTGAGCGATGGCAACAAGGTGCTTTTAACACTATTCCTGCATTTATA
GAAAGGGAGCTAGCAAAAAT

Downstream 100 bases:

>100_bases
AAAATTTGCATTTAAGTTTTCATTCAACTAATACCTTTCCAAAATCAAGTTGTTGATGAACAAATTTTTCAGAAATCTTT
GGGAAATTAGGTTCTTTTTT

Product: arginase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKSNLKDLSLFNNDGGIFLGAQRGIDQCRVSLLGVPYDGTCCFRPGARFGPSAVREDSYGIETYCPQLNLDLEDIKFAD
IGSLDVPLGDAKLTLDYIKDATNILLKNNLKPLIIGGEHSITSGIIKSIITKYPDLIMLQLDAHADLRDEWLGSKFSHAC
TMKRCLEILPSKKIFQIGIRSGTKSEFLEMNNSKRLIQHTLGENAKSLEEALKSFKGRPIYLTFDLDWFDPSVMPGTGTP
EPGGYFWGDFAAIINVIKSHNLIGADVVELSPKLDNTGISSILAAKVVRSLILLLDKSS

Sequences:

>Translated_299_residues
MTKSNLKDLSLFNNDGGIFLGAQRGIDQCRVSLLGVPYDGTCCFRPGARFGPSAVREDSYGIETYCPQLNLDLEDIKFAD
IGSLDVPLGDAKLTLDYIKDATNILLKNNLKPLIIGGEHSITSGIIKSIITKYPDLIMLQLDAHADLRDEWLGSKFSHAC
TMKRCLEILPSKKIFQIGIRSGTKSEFLEMNNSKRLIQHTLGENAKSLEEALKSFKGRPIYLTFDLDWFDPSVMPGTGTP
EPGGYFWGDFAAIINVIKSHNLIGADVVELSPKLDNTGISSILAAKVVRSLILLLDKSS
>Mature_298_residues
TKSNLKDLSLFNNDGGIFLGAQRGIDQCRVSLLGVPYDGTCCFRPGARFGPSAVREDSYGIETYCPQLNLDLEDIKFADI
GSLDVPLGDAKLTLDYIKDATNILLKNNLKPLIIGGEHSITSGIIKSIITKYPDLIMLQLDAHADLRDEWLGSKFSHACT
MKRCLEILPSKKIFQIGIRSGTKSEFLEMNNSKRLIQHTLGENAKSLEEALKSFKGRPIYLTFDLDWFDPSVMPGTGTPE
PGGYFWGDFAAIINVIKSHNLIGADVVELSPKLDNTGISSILAAKVVRSLILLLDKSS

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=276, Percent_Identity=33.695652173913, Blast_Score=157, Evalue=8e-39,
Organism=Escherichia coli, GI1789306, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325146, Length=206, Percent_Identity=31.0679611650485, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32854; Mature: 32723

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSNLKDLSLFNNDGGIFLGAQRGIDQCRVSLLGVPYDGTCCFRPGARFGPSAVREDSY
CCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHCCCCC
GIETYCPQLNLDLEDIKFADIGSLDVPLGDAKLTLDYIKDATNILLKNNLKPLIIGGEHS
CCHHCCCCCCCCHHHCCEECCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEECCCHH
ITSGIIKSIITKYPDLIMLQLDAHADLRDEWLGSKFSHACTMKRCLEILPSKKIFQIGIR
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECC
SGTKSEFLEMNNSKRLIQHTLGENAKSLEEALKSFKGRPIYLTFDLDWFDPSVMPGTGTP
CCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCC
EPGGYFWGDFAAIINVIKSHNLIGADVVELSPKLDNTGISSILAAKVVRSLILLLDKSS
CCCCEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKSNLKDLSLFNNDGGIFLGAQRGIDQCRVSLLGVPYDGTCCFRPGARFGPSAVREDSY
CCCCCCCEEEEECCCCEEEECCCCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHCCCCC
GIETYCPQLNLDLEDIKFADIGSLDVPLGDAKLTLDYIKDATNILLKNNLKPLIIGGEHS
CCHHCCCCCCCCHHHCCEECCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEECCCHH
ITSGIIKSIITKYPDLIMLQLDAHADLRDEWLGSKFSHACTMKRCLEILPSKKIFQIGIR
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECC
SGTKSEFLEMNNSKRLIQHTLGENAKSLEEALKSFKGRPIYLTFDLDWFDPSVMPGTGTP
CCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCC
EPGGYFWGDFAAIINVIKSHNLIGADVVELSPKLDNTGISSILAAKVVRSLILLLDKSS
CCCCEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]