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The map label for this gene is speE

Identifier: 124026863

GI number: 124026863

Start: 1814971

End: 1815837

Strand: Direct

Name: speE

Synonym: NATL1_21581

Alternate gene names: 124026863

Gene position: 1814971-1815837 (Clockwise)

Preceding gene: 124026862

Following gene: 124026864

Centisome position: 97.33

GC content: 34.6

Gene sequence:

>867_bases
ATGAATTACAAACTAAAAACAAGGTCAGGGTGGCTTGACGAGTATCATCAAGGCGTTAGATATGGACTAGAGGGAAAATT
AATACTAGAAGAGTCTAGCCCTTTTCAAAAAATTACCATATATGAAAGTAAAAGATATGGGAAAGCACTATTACTTGATG
ATTGCTGGATGACTGCAGAAAAGTCTGAAAAGTGTTATCACGAATGTCTTATTCACCCTGCCTTGTGTTGTTCTACACGA
ATAGATAATATTTTAATTATTGGAGGAGGTGATGGGGGTAGTGCAAGAGAATGTTTAAAATACAAAGAGGTTAAGTCTAT
CGATTTAGTTGAAATTGATCTAAGAGTTATTGAATTAAGTCAAAAATATTTACCTACTATTGGAGGAAATGCATGGTCTG
ATTCACGATTAAATTTACAAATCAAAAATGGAATTGATTGGGTAAAGCATACAAAAGAGAATTCATATGATGTAATTATT
ATTGATGGAGCAGATCCTATTGGACCGTCCAAAGAATTATTCAGCAATTCTTTTCTGAAAGATTGCAAACGAATACTTAA
ACCAGGAGGGGTTTTGGCAACACAAAGTGAATCTCCAGAGTCTTTTCAAGAAATTCACATAAATATTGTCAAAATTCTTC
GTGAAATTTTTGACTACGCTGATCCAATGTATGGATCTGTATCCATATACCCAAGCGGTTTATGGAGCTGGGCATTTGCA
TCTATGGAGAATCCAAAATATATATATCCAAAAAAAAGTCGCGTAAAAGAGATCTCCGAAAATTGTCAGGTTTGGAGTGA
GCGATGGCAACAAGGTGCTTTTAACACTATTCCTGCATTTATAGAAAGGGAGCTAGCAAAAATATGA

Upstream 100 bases:

>100_bases
GAAGAAAATATAGATGGCGAATTACATTCCCAATCAAAAAAGAAATTAGAAAAATATTGGAAATTAGCGAAGATCAATCT
CAAGCATTAATATCATAATT

Downstream 100 bases:

>100_bases
CCAAATCCAACCTCAAAGATCTATCACTATTTAATAACGATGGTGGAATATTTTTGGGCGCCCAAAGAGGCATCGATCAA
TGCAGAGTGTCACTTTTAGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNYKLKTRSGWLDEYHQGVRYGLEGKLILEESSPFQKITIYESKRYGKALLLDDCWMTAEKSEKCYHECLIHPALCCSTR
IDNILIIGGGDGGSARECLKYKEVKSIDLVEIDLRVIELSQKYLPTIGGNAWSDSRLNLQIKNGIDWVKHTKENSYDVII
IDGADPIGPSKELFSNSFLKDCKRILKPGGVLATQSESPESFQEIHINIVKILREIFDYADPMYGSVSIYPSGLWSWAFA
SMENPKYIYPKKSRVKEISENCQVWSERWQQGAFNTIPAFIERELAKI

Sequences:

>Translated_288_residues
MNYKLKTRSGWLDEYHQGVRYGLEGKLILEESSPFQKITIYESKRYGKALLLDDCWMTAEKSEKCYHECLIHPALCCSTR
IDNILIIGGGDGGSARECLKYKEVKSIDLVEIDLRVIELSQKYLPTIGGNAWSDSRLNLQIKNGIDWVKHTKENSYDVII
IDGADPIGPSKELFSNSFLKDCKRILKPGGVLATQSESPESFQEIHINIVKILREIFDYADPMYGSVSIYPSGLWSWAFA
SMENPKYIYPKKSRVKEISENCQVWSERWQQGAFNTIPAFIERELAKI
>Mature_288_residues
MNYKLKTRSGWLDEYHQGVRYGLEGKLILEESSPFQKITIYESKRYGKALLLDDCWMTAEKSEKCYHECLIHPALCCSTR
IDNILIIGGGDGGSARECLKYKEVKSIDLVEIDLRVIELSQKYLPTIGGNAWSDSRLNLQIKNGIDWVKHTKENSYDVII
IDGADPIGPSKELFSNSFLKDCKRILKPGGVLATQSESPESFQEIHINIVKILREIFDYADPMYGSVSIYPSGLWSWAFA
SMENPKYIYPKKSRVKEISENCQVWSERWQQGAFNTIPAFIERELAKI

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=294, Percent_Identity=31.2925170068027, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1786312, Length=286, Percent_Identity=29.7202797202797, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25154106, Length=263, Percent_Identity=32.319391634981, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI212645616, Length=212, Percent_Identity=35.377358490566, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6325326, Length=269, Percent_Identity=30.4832713754647, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323175, Length=239, Percent_Identity=34.7280334728033, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24645443, Length=284, Percent_Identity=32.3943661971831, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI21355941, Length=212, Percent_Identity=33.0188679245283, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_PROM1 (A2C5F4)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015978.1
- ProteinModelPortal:   A2C5F4
- SMR:   A2C5F4
- STRING:   A2C5F4
- GeneID:   4780587
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21581
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   WSWTFAS
- ProtClustDB:   PRK00811
- BioCyc:   PMAR167555:NATL1_21581-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32935; Mature: 32935

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 36-36 BINDING 91-91 BINDING 111-111 BINDING 162-162 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKLKTRSGWLDEYHQGVRYGLEGKLILEESSPFQKITIYESKRYGKALLLDDCWMTAE
CCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEHHHHCCCH
KSEKCYHECLIHPALCCSTRIDNILIIGGGDGGSARECLKYKEVKSIDLVEIDLRVIELS
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHEEHH
QKYLPTIGGNAWSDSRLNLQIKNGIDWVKHTKENSYDVIIIDGADPIGPSKELFSNSFLK
HHHCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
DCKRILKPGGVLATQSESPESFQEIHINIVKILREIFDYADPMYGSVSIYPSGLWSWAFA
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHH
SMENPKYIYPKKSRVKEISENCQVWSERWQQGAFNTIPAFIERELAKI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNYKLKTRSGWLDEYHQGVRYGLEGKLILEESSPFQKITIYESKRYGKALLLDDCWMTAE
CCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEHHHHCCCH
KSEKCYHECLIHPALCCSTRIDNILIIGGGDGGSARECLKYKEVKSIDLVEIDLRVIELS
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHEEHH
QKYLPTIGGNAWSDSRLNLQIKNGIDWVKHTKENSYDVIIIDGADPIGPSKELFSNSFLK
HHHCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
DCKRILKPGGVLATQSESPESFQEIHINIVKILREIFDYADPMYGSVSIYPSGLWSWAFA
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHH
SMENPKYIYPKKSRVKEISENCQVWSERWQQGAFNTIPAFIERELAKI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA