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The map label for this gene is thiG

Identifier: 124026838

GI number: 124026838

Start: 1790422

End: 1791228

Strand: Direct

Name: thiG

Synonym: NATL1_21331

Alternate gene names: 124026838

Gene position: 1790422-1791228 (Clockwise)

Preceding gene: 124026837

Following gene: 124026839

Centisome position: 96.02

GC content: 38.04

Gene sequence:

>807_bases
ATGCAAAAAACAAATAAATTTCTAAAAATAGGAACTAAAGAGTTCAAAAGTCGACTTCTTGTTGGAACTGGTAAATACTC
ATCCCTAGAAGTCATGCAAAAAAGCCTAATAAATACAAAATGTGAAATAGTTACTGTCGCAGTTAGAAGAGTGCAAGGGC
TAGAACATGGACATAAAGGATTAATGGAATCAATAGACTGGAAAAGAATATGGATGCTGCCAAACACTGCAGGATGCTCA
AATGCCGAAGAAGCTATTCGAATAGCAAAACTTGGCAGAGAATTAGCAAAGTTAGCAGGTCAAGAAAATAATAATTTTGT
CAAATTAGAGGTTATTCCTGACAAAAAATATTTATTACCCGACCCAATTGGAACCTTAAAAGCAGCCGAACAATTAGTTA
AAGAAGGATTTACTGTTCTTCCATATATAAATTCTGATCCATTAATTGCAAAACAGCTTGAAGAAATTGGATGTGCAACC
GTTATGCCTCTTGGTTCCCCCATTGGATCTGCTCAAGGAATAAGAAATGCTGCAAACATTGCCATGATTATCGCAGAATC
TCGAATCCCAATAATTATTGATGCGGGCATTGGAGTTCCAAGTGAAGCAGCGCAAGCATTAGAAATGGGTGCTGATGGGG
TTCTAATAAATAGTGCTATTGCCTTAGCTGAAAACCCAATTCTTATGGCTCAAGCCTTCTCTAAGGCTACCGAAGCAGGC
AGGGATGGTTATCTGTCTGGAAGACTGAAAGAGAACCCTCTTGCTAGTCCAAGCTCTCCACTAGAAGGAGTTATTTCAAA
TAATTAG

Upstream 100 bases:

>100_bases
TCTCAAATTAAAAGACTTGAACGTATAGTTGGAAAAGATAAAGATAAATTATCGAATAAAAAAGGCTTCTAAATCATTAT
TAATCCATCATGTTTGAACC

Downstream 100 bases:

>100_bases
AGAAATTCGTTAAAAATTAGTAACGTAATAGGCTAATAAACAAAAAGACATGGCAGTCACTAGAGAAAGTCAAGGCAGGC
TTAACGTTTTTGCGGATGAG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQKTNKFLKIGTKEFKSRLLVGTGKYSSLEVMQKSLINTKCEIVTVAVRRVQGLEHGHKGLMESIDWKRIWMLPNTAGCS
NAEEAIRIAKLGRELAKLAGQENNNFVKLEVIPDKKYLLPDPIGTLKAAEQLVKEGFTVLPYINSDPLIAKQLEEIGCAT
VMPLGSPIGSAQGIRNAANIAMIIAESRIPIIIDAGIGVPSEAAQALEMGADGVLINSAIALAENPILMAQAFSKATEAG
RDGYLSGRLKENPLASPSSPLEGVISNN

Sequences:

>Translated_268_residues
MQKTNKFLKIGTKEFKSRLLVGTGKYSSLEVMQKSLINTKCEIVTVAVRRVQGLEHGHKGLMESIDWKRIWMLPNTAGCS
NAEEAIRIAKLGRELAKLAGQENNNFVKLEVIPDKKYLLPDPIGTLKAAEQLVKEGFTVLPYINSDPLIAKQLEEIGCAT
VMPLGSPIGSAQGIRNAANIAMIIAESRIPIIIDAGIGVPSEAAQALEMGADGVLINSAIALAENPILMAQAFSKATEAG
RDGYLSGRLKENPLASPSSPLEGVISNN
>Mature_268_residues
MQKTNKFLKIGTKEFKSRLLVGTGKYSSLEVMQKSLINTKCEIVTVAVRRVQGLEHGHKGLMESIDWKRIWMLPNTAGCS
NAEEAIRIAKLGRELAKLAGQENNNFVKLEVIPDKKYLLPDPIGTLKAAEQLVKEGFTVLPYINSDPLIAKQLEEIGCAT
VMPLGSPIGSAQGIRNAANIAMIIAESRIPIIIDAGIGVPSEAAQALEMGADGVLINSAIALAENPILMAQAFSKATEAG
RDGYLSGRLKENPLASPSSPLEGVISNN

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=259, Percent_Identity=47.8764478764479, Blast_Score=244, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_PROM1 (A2C5C9)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015953.1
- ProteinModelPortal:   A2C5C9
- SMR:   A2C5C9
- STRING:   A2C5C9
- GeneID:   4780876
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21331
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   PIIIDAG
- ProtClustDB:   PRK00208
- BioCyc:   PMAR167555:NATL1_21331-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28730; Mature: 28730

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: ACT_SITE 108-108 BINDING 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTNKFLKIGTKEFKSRLLVGTGKYSSLEVMQKSLINTKCEIVTVAVRRVQGLEHGHKG
CCCCCHHHHCCHHHHHCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LMESIDWKRIWMLPNTAGCSNAEEAIRIAKLGRELAKLAGQENNNFVKLEVIPDKKYLLP
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DPIGTLKAAEQLVKEGFTVLPYINSDPLIAKQLEEIGCATVMPLGSPIGSAQGIRNAANI
CCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCE
AMIIAESRIPIIIDAGIGVPSEAAQALEMGADGVLINSAIALAENPILMAQAFSKATEAG
EEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCC
RDGYLSGRLKENPLASPSSPLEGVISNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKTNKFLKIGTKEFKSRLLVGTGKYSSLEVMQKSLINTKCEIVTVAVRRVQGLEHGHKG
CCCCCHHHHCCHHHHHCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LMESIDWKRIWMLPNTAGCSNAEEAIRIAKLGRELAKLAGQENNNFVKLEVIPDKKYLLP
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DPIGTLKAAEQLVKEGFTVLPYINSDPLIAKQLEEIGCATVMPLGSPIGSAQGIRNAANI
CCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCE
AMIIAESRIPIIIDAGIGVPSEAAQALEMGADGVLINSAIALAENPILMAQAFSKATEAG
EEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCC
RDGYLSGRLKENPLASPSSPLEGVISNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA