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The map label for this gene is mesJ

Identifier: 124026824

GI number: 124026824

Start: 1775514

End: 1776494

Strand: Direct

Name: mesJ

Synonym: NATL1_21191

Alternate gene names: 124026824

Gene position: 1775514-1776494 (Clockwise)

Preceding gene: 124026821

Following gene: 124026828

Centisome position: 95.22

GC content: 31.7

Gene sequence:

>981_bases
ATGCGTTTACACAAAAGACTGAAACAAAATAAATCTCTGCTTCCCAGTAACTCCACCATTCTTTTAGCCATATCTGGTGG
CCAAGATTCGATGGCCCTTCTCAAATTAATTATTGATTTAAAAAGACTGCATAAATGGCAAGTCGAAATCTGGCATGGAA
ATCACCAATGGCATTCTAAATCCGAGAAAACAGAAGAAGAGCTCAAACTATGGTGTCTGAAAAATCAAATTTCATTTCAC
TCTAATAAAGCAGATAAAAAAGAAGTCGCTAATGAAGAAAAGGCAAGAGATTGGAGATATAAAAATCTAATAATGAAAGC
TAAGTTTTTATCATCAAAAAATATACATTTTCCGTGTACAAGAATTTTAACTGGTCATACAGCTACTGATCGAGCAGAGA
CAGTCATTATGAACTTAGCCAGAGGGACTGATCTGACTGGACTTACTACTCTTAAGGAGCAAAGAACTATAGAGAGTAAT
ATAGATTTAACCAGACCGCTACTGATTTTCAATAGAAATGAAACTCTCGAAATTTGTAAAGATTTTAACTTACCAATTTG
GATTGATCCTTCAAATGAAAATATTAATTTAACAAGAAATAAAATAAGAAAAGAAATTTTACCTATTTTAAATTCGATCT
ATAAGGGAGCAGACTCAAGAATAGCCTCAGTAGCTAATAGACTTGAAAGTTATAACGAAGACCAAAAATTATTTGCAAAA
ATAGCAATACAATTTTGCCAAGGAGAAAAAATCAATTCTCTATCGAGAATCAAACTATTCGGCTTAACAAACTCTATTAG
ACAAATAATTCTTTCTAATTGGTTAAAGACAATGGGTGTAAAAAGGATAACAGCTTTGCAAATTGAAGAAATAAATACAA
AAGTATCTCAAAGAAAACCACCTGGAAGTATCCATCTTCATGGAGACTTCCTAATACGTTGGAACAAAGAAGCTATTTAT
ATCTCAAATAAAACTAATTAA

Upstream 100 bases:

>100_bases
ATTGACTCAGCAAAAGAACTAAAAAAATGGGAATAGAGCTCTGAAACCAAAGCAAATGTCACAATCTTCTCAAAATGACA
GGTCTTGGAGTAAATGGCAT

Downstream 100 bases:

>100_bases
AATAAACAATTTTTTTGTTATCAGCTAATTGCTGATCTGCGACGTCTTGTTCTTCCTGTGGGCATTTCCTCAGCATTAGG
TTCCTTATTTGTTTTTTGTT

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRLHKRLKQNKSLLPSNSTILLAISGGQDSMALLKLIIDLKRLHKWQVEIWHGNHQWHSKSEKTEEELKLWCLKNQISFH
SNKADKKEVANEEKARDWRYKNLIMKAKFLSSKNIHFPCTRILTGHTATDRAETVIMNLARGTDLTGLTTLKEQRTIESN
IDLTRPLLIFNRNETLEICKDFNLPIWIDPSNENINLTRNKIRKEILPILNSIYKGADSRIASVANRLESYNEDQKLFAK
IAIQFCQGEKINSLSRIKLFGLTNSIRQIILSNWLKTMGVKRITALQIEEINTKVSQRKPPGSIHLHGDFLIRWNKEAIY
ISNKTN

Sequences:

>Translated_326_residues
MRLHKRLKQNKSLLPSNSTILLAISGGQDSMALLKLIIDLKRLHKWQVEIWHGNHQWHSKSEKTEEELKLWCLKNQISFH
SNKADKKEVANEEKARDWRYKNLIMKAKFLSSKNIHFPCTRILTGHTATDRAETVIMNLARGTDLTGLTTLKEQRTIESN
IDLTRPLLIFNRNETLEICKDFNLPIWIDPSNENINLTRNKIRKEILPILNSIYKGADSRIASVANRLESYNEDQKLFAK
IAIQFCQGEKINSLSRIKLFGLTNSIRQIILSNWLKTMGVKRITALQIEEINTKVSQRKPPGSIHLHGDFLIRWNKEAIY
ISNKTN
>Mature_326_residues
MRLHKRLKQNKSLLPSNSTILLAISGGQDSMALLKLIIDLKRLHKWQVEIWHGNHQWHSKSEKTEEELKLWCLKNQISFH
SNKADKKEVANEEKARDWRYKNLIMKAKFLSSKNIHFPCTRILTGHTATDRAETVIMNLARGTDLTGLTTLKEQRTIESN
IDLTRPLLIFNRNETLEICKDFNLPIWIDPSNENINLTRNKIRKEILPILNSIYKGADSRIASVANRLESYNEDQKLFAK
IAIQFCQGEKINSLSRIKLFGLTNSIRQIILSNWLKTMGVKRITALQIEEINTKVSQRKPPGSIHLHGDFLIRWNKEAIY
ISNKTN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PROM1 (A2C5B5)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015939.1
- ProteinModelPortal:   A2C5B5
- SMR:   A2C5B5
- STRING:   A2C5B5
- GeneID:   4780140
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21191
- eggNOG:   COG0037
- HOGENOM:   HBG692699
- OMA:   LIFSRED
- ProtClustDB:   CLSK716057
- BioCyc:   PMAR167555:NATL1_21191-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 37782; Mature: 37782

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHKRLKQNKSLLPSNSTILLAISGGQDSMALLKLIIDLKRLHKWQVEIWHGNHQWHSK
CCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
SEKTEEELKLWCLKNQISFHSNKADKKEVANEEKARDWRYKNLIMKAKFLSSKNIHFPCT
CCCCHHHHHHHHHHCHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RILTGHTATDRAETVIMNLARGTDLTGLTTLKEQRTIESNIDLTRPLLIFNRNETLEICK
HHEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
DFNLPIWIDPSNENINLTRNKIRKEILPILNSIYKGADSRIASVANRLESYNEDQKLFAK
CCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
IAIQFCQGEKINSLSRIKLFGLTNSIRQIILSNWLKTMGVKRITALQIEEINTKVSQRKP
HHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHCCCC
PGSIHLHGDFLIRWNKEAIYISNKTN
CCEEEEEEEEEEEECCEEEEEECCCC
>Mature Secondary Structure
MRLHKRLKQNKSLLPSNSTILLAISGGQDSMALLKLIIDLKRLHKWQVEIWHGNHQWHSK
CCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
SEKTEEELKLWCLKNQISFHSNKADKKEVANEEKARDWRYKNLIMKAKFLSSKNIHFPCT
CCCCHHHHHHHHHHCHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RILTGHTATDRAETVIMNLARGTDLTGLTTLKEQRTIESNIDLTRPLLIFNRNETLEICK
HHEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
DFNLPIWIDPSNENINLTRNKIRKEILPILNSIYKGADSRIASVANRLESYNEDQKLFAK
CCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
IAIQFCQGEKINSLSRIKLFGLTNSIRQIILSNWLKTMGVKRITALQIEEINTKVSQRKP
HHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHCCCC
PGSIHLHGDFLIRWNKEAIYISNKTN
CCEEEEEEEEEEEECCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA