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The map label for this gene is ribH

Identifier: 124026816

GI number: 124026816

Start: 1765195

End: 1765671

Strand: Reverse

Name: ribH

Synonym: NATL1_21111

Alternate gene names: 124026816

Gene position: 1765671-1765195 (Counterclockwise)

Preceding gene: 124026817

Following gene: 124026814

Centisome position: 94.69

GC content: 39.41

Gene sequence:

>477_bases
ATGGCGACAATAGAGGGTACCTTTGAAAAATCATCCTCATTAAAAGTAGCTATTGTTATAGCTAGATTTAATGATCTAAT
TACTAATAAGCTCTTAAGCGGATGTTTAGATTGTTTGTCACGTCACGGTATTGATGTCTCAGAGTCAAGTAATCAAATTG
ATGTGGCATGGGTTCCAGGTTCTTTTGAATTGCCAATTATTTCTCAAGAACTTGCTAGAACGGGAAAATATGAGGTGTTA
ATTACATTGGGTGCAGTGATAAGAGGAGATACTCCTCACTTTGATGTTGTTGTATCAGAGGCGAGCAAAGGGATAGCTAC
TGTATCAAGAGAAACAGGAGTACCAATTATCTTCGGGGTCTTAACTACTGATACTATGCAGCAAGCATTGGAGCGGGCGG
GAATTAAAAATAATCTTGGGTGGAGTTATGCTTTGCAAGCCCTCGAGATGGGTTCTTTAATGAAGGCGGTGACTTAA

Upstream 100 bases:

>100_bases
ATGATTTAAGATAACTGCTTATATAGACTAATGTTGAATTTGATGTTTTAAAATTACTCGCAAGAGGTTACTGTTAGTAG
GTATTAAAAGCCTTTAAAAA

Downstream 100 bases:

>100_bases
ATAATCTTGTCAATTGCGAGAATTATTTGACCACAACACCTCTTTTGAGGCATGCTTAATACATTAACCATTAACTATGT
TTGTGCGGATGTAGCTCAGT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MATIEGTFEKSSSLKVAIVIARFNDLITNKLLSGCLDCLSRHGIDVSESSNQIDVAWVPGSFELPIISQELARTGKYEVL
ITLGAVIRGDTPHFDVVVSEASKGIATVSRETGVPIIFGVLTTDTMQQALERAGIKNNLGWSYALQALEMGSLMKAVT

Sequences:

>Translated_158_residues
MATIEGTFEKSSSLKVAIVIARFNDLITNKLLSGCLDCLSRHGIDVSESSNQIDVAWVPGSFELPIISQELARTGKYEVL
ITLGAVIRGDTPHFDVVVSEASKGIATVSRETGVPIIFGVLTTDTMQQALERAGIKNNLGWSYALQALEMGSLMKAVT
>Mature_157_residues
ATIEGTFEKSSSLKVAIVIARFNDLITNKLLSGCLDCLSRHGIDVSESSNQIDVAWVPGSFELPIISQELARTGKYEVLI
TLGAVIRGDTPHFDVVVSEASKGIATVSRETGVPIIFGVLTTDTMQQALERAGIKNNLGWSYALQALEMGSLMKAVT

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=144, Percent_Identity=50, Blast_Score=142, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=32.8859060402685, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_PROM1 (A2C5A7)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015931.1
- ProteinModelPortal:   A2C5A7
- SMR:   A2C5A7
- STRING:   A2C5A7
- GeneID:   4781093
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21111
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   PMAR167555:NATL1_21111-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16957; Mature: 16826

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIEGTFEKSSSLKVAIVIARFNDLITNKLLSGCLDCLSRHGIDVSESSNQIDVAWVPG
CCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
SFELPIISQELARTGKYEVLITLGAVIRGDTPHFDVVVSEASKGIATVSRETGVPIIFGV
CCCCCCHHHHHHCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCEEECCCCCCCEEEEE
LTTDTMQQALERAGIKNNLGWSYALQALEMGSLMKAVT
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATIEGTFEKSSSLKVAIVIARFNDLITNKLLSGCLDCLSRHGIDVSESSNQIDVAWVPG
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
SFELPIISQELARTGKYEVLITLGAVIRGDTPHFDVVVSEASKGIATVSRETGVPIIFGV
CCCCCCHHHHHHCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCEEECCCCCCCEEEEE
LTTDTMQQALERAGIKNNLGWSYALQALEMGSLMKAVT
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA