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The map label for this gene is rfbA [H]

Identifier: 124026814

GI number: 124026814

Start: 1763544

End: 1764434

Strand: Reverse

Name: rfbA [H]

Synonym: NATL1_21091

Alternate gene names: 124026814

Gene position: 1764434-1763544 (Counterclockwise)

Preceding gene: 124026816

Following gene: 124026813

Centisome position: 94.62

GC content: 34.79

Gene sequence:

>891_bases
ATGAAACGTAAAGGAATTATTCTCGCTGGCGGAACTGGTTCGCGTCTGGCGCCATTAACTAATTCAGTAAGCAAGCAGCT
ACTTCCTGTTTACGATAAACCGATGATTTTCTATCCTTTATCAACCTTAATGTTGGCTGGGATTAGAGAAATTTTAATAA
TTACTACTCCTATCGATAGGAATCAATTTGAAAGATTGCTTGGTAATGGATCAGATTTTGGGATATCTATAAAATATGAA
GAACAGCAATCTCCTGAGGGTATTGCACAAGCATTTTTGATTGGTGAAAAGTTTTTAAATGGTTCTCCGGTTGCTTTGAT
TTTAGGTGACAATCTTTTCCATGGTCATGAATTTAGAAAACAATTAAACAAGGCTAATAATCTAATTGAATTATCTACTA
TTTTTGCTTATCCAGTCATAGATCCTCAGAGATATGGTGTAGTTGTATTTGATAATGAAGGTAATGCCATAGAGATTGAG
GAGAAACCTGAAATACCATCTAGCCGGTTCGCTATTACTGGCTTATATTTTTACGATTCATCAATAGTTGATAAAGCTAA
AAAAATCTCTCCCTCATCAAGGGATGAACTAGAGATCACATCAATTAATCAGCTATATCTTCAAGAGAGACGTTTGAAAG
TTGAGTTAATGGGGAGAGGAATGGCTTGGTTAGACACCGGTACATTCGATTCCTTGCAAGAAGCAGGCGCATTTATTAAA
ACACTCGAAAATAGACAAGGATTAAAAATAGGATCTCCTGAGGAAGTTGCTTGGAGAAATTGTTGGATAGACAAGGATCA
ATTAAAGAAGAAAGCAAATCAATACATGAACAGTGGTTATGGTGATTACTTATTTGAATTAATTTCTAAGCCTCATAGTA
ATTTATTTTAA

Upstream 100 bases:

>100_bases
AATCCTAATATTCTTAATCCTGGAGCTCCGGGTGCATGTTTAACAATTTCAATAGCGTTCAAATCTTTGCTTTTTTTCCT
CTTGAAGATAACCTAATTTT

Downstream 100 bases:

>100_bases
AAGGATTTTGATATTAATAACATGAAAATTCAATTTTTATATTCTAATAAAATTTAAAAATTCGGTGAATTATGAATAAT
ATTGAATATTAATTCAATAT

Product: dTDP-glucose pyrophosphorylase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKRKGIILAGGTGSRLAPLTNSVSKQLLPVYDKPMIFYPLSTLMLAGIREILIITTPIDRNQFERLLGNGSDFGISIKYE
EQQSPEGIAQAFLIGEKFLNGSPVALILGDNLFHGHEFRKQLNKANNLIELSTIFAYPVIDPQRYGVVVFDNEGNAIEIE
EKPEIPSSRFAITGLYFYDSSIVDKAKKISPSSRDELEITSINQLYLQERRLKVELMGRGMAWLDTGTFDSLQEAGAFIK
TLENRQGLKIGSPEEVAWRNCWIDKDQLKKKANQYMNSGYGDYLFELISKPHSNLF

Sequences:

>Translated_296_residues
MKRKGIILAGGTGSRLAPLTNSVSKQLLPVYDKPMIFYPLSTLMLAGIREILIITTPIDRNQFERLLGNGSDFGISIKYE
EQQSPEGIAQAFLIGEKFLNGSPVALILGDNLFHGHEFRKQLNKANNLIELSTIFAYPVIDPQRYGVVVFDNEGNAIEIE
EKPEIPSSRFAITGLYFYDSSIVDKAKKISPSSRDELEITSINQLYLQERRLKVELMGRGMAWLDTGTFDSLQEAGAFIK
TLENRQGLKIGSPEEVAWRNCWIDKDQLKKKANQYMNSGYGDYLFELISKPHSNLF
>Mature_296_residues
MKRKGIILAGGTGSRLAPLTNSVSKQLLPVYDKPMIFYPLSTLMLAGIREILIITTPIDRNQFERLLGNGSDFGISIKYE
EQQSPEGIAQAFLIGEKFLNGSPVALILGDNLFHGHEFRKQLNKANNLIELSTIFAYPVIDPQRYGVVVFDNEGNAIEIE
EKPEIPSSRFAITGLYFYDSSIVDKAKKISPSSRDELEITSINQLYLQERRLKVELMGRGMAWLDTGTFDSLQEAGAFIK
TLENRQGLKIGSPEEVAWRNCWIDKDQLKKKANQYMNSGYGDYLFELISKPHSNLF

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=256, Percent_Identity=24.21875, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI11761619, Length=256, Percent_Identity=24.21875, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=61.0526315789474, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788351, Length=285, Percent_Identity=58.2456140350877, Blast_Score=350, Evalue=5e-98,
Organism=Escherichia coli, GI1787488, Length=253, Percent_Identity=25.296442687747, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133931050, Length=285, Percent_Identity=25.2631578947368, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=25.4980079681275, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=25.4980079681275, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 33363; Mature: 33363

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKGIILAGGTGSRLAPLTNSVSKQLLPVYDKPMIFYPLSTLMLAGIREILIITTPIDR
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCH
NQFERLLGNGSDFGISIKYEEQQSPEGIAQAFLIGEKFLNGSPVALILGDNLFHGHEFRK
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHH
QLNKANNLIELSTIFAYPVIDPQRYGVVVFDNEGNAIEIEEKPEIPSSRFAITGLYFYDS
HHHHHCCEEEEEHEEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCEEEEEEEEECC
SIVDKAKKISPSSRDELEITSINQLYLQERRLKVELMGRGMAWLDTGTFDSLQEAGAFIK
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHEEEEEECCCEEEEECCCHHHHHHHHHHHH
TLENRQGLKIGSPEEVAWRNCWIDKDQLKKKANQYMNSGYGDYLFELISKPHSNLF
HHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRKGIILAGGTGSRLAPLTNSVSKQLLPVYDKPMIFYPLSTLMLAGIREILIITTPIDR
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCH
NQFERLLGNGSDFGISIKYEEQQSPEGIAQAFLIGEKFLNGSPVALILGDNLFHGHEFRK
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHH
QLNKANNLIELSTIFAYPVIDPQRYGVVVFDNEGNAIEIEEKPEIPSSRFAITGLYFYDS
HHHHHCCEEEEEHEEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCEEEEEEEEECC
SIVDKAKKISPSSRDELEITSINQLYLQERRLKVELMGRGMAWLDTGTFDSLQEAGAFIK
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHEEEEEECCCEEEEECCCHHHHHHHHHHHH
TLENRQGLKIGSPEEVAWRNCWIDKDQLKKKANQYMNSGYGDYLFELISKPHSNLF
HHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]