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The map label for this gene is hemF

Identifier: 124026733

GI number: 124026733

Start: 1674087

End: 1675133

Strand: Direct

Name: hemF

Synonym: NATL1_20281

Alternate gene names: 124026733

Gene position: 1674087-1675133 (Clockwise)

Preceding gene: 124026722

Following gene: 124026736

Centisome position: 89.78

GC content: 40.21

Gene sequence:

>1047_bases
TTGTCCTCCTCACAAGATAAAGCCAATTTACCGGCAAACAATTCAAGAGCTCGAGCAAAAAAGCTTGTTTTAGAGCTTCA
AGATGAAATTTGCGCTGGCTTAGAGACTATTGATGGTGAAGGCAAATTCCTAGAAGAATCTTGGGAGAGACCAGAGGGTG
GTGGTGGACGCTCAAGAGTCTTGAAAGATGGAAAAATCTTTGAGCAGGGAGGGGTGAATTTTTCTGAAGTACATGGCAAT
GAACTTCCACCCTCAATCATTAGCCAAAGACCAGAAGCAAAAGGTCACTCTTGGTTTGCGACAGGAACCTCAATGGTTCT
TCACCCCAAAAATCCTTACATACCTACTGTTCATCTTAATTACAGATATTTCGAAGCAGGTCCCGTTTGGTGGTTTGGGG
GAGGGGCTGATTTAACACCTTTCTATCCATATCTATCTGACACGCGTCACTTTCACTCATGCCATAAAAATGCATGTGAC
ACAATTGACAAAGATCTACATAAGGTTTTCAAACCTTGGTGTGACGAATATTTCTTTCTAAAACATAGAAATGAGACTAG
AGGAGTTGGAGGTATCTTTTATGACTATCAAGACGGATCCGGCTTGCTTTATAAAGGACAAAATGCCAATGGGAAGGCCT
CTAAAATCGCAAAAGAGCTAGGGGAATATTCTTTGAATTGGGAAAATCTTTTTTCACTTGCCAAAGCATGCGGGCAGGCA
TTTCTGCCCTCCTATGAGCCAATAATAAAAAAGCGAAAAAATCAAAGCTTTTCAACCAAAGAACGAGACTTTCAACTTTA
TAGGCGAGGTAGATATGCTGAGTTCAATTTGGTATGGGATAGAGGAACCATTTTTGGATTACAAACGAATGGAAGAACGG
AGTCAATATTGATGTCATTACCTCCCTTAGCAAGATGGGAGTATGGATATAAGCCAGAAGAAAATTCACGTGAGGCTCTA
TTAACAGATTTATTTACTAAGCCTCAAGATTGGTTTACAGATAAATCTCTGGAAAAGAGGTGTTTAACTCATCAAGCATT
GGATTAG

Upstream 100 bases:

>100_bases
AATGAGTAAAAACTATTCGCAATACAAACTTTTCCAATATTGGTGTGAAATGTGCATCATTTGAAAGTATTTTTTTAAAG
AAAGCAAAATACAATTAATT

Downstream 100 bases:

>100_bases
ATAGTGAACTTCTAGATTATTCCATTTAAAAAAGCCTGAACTATTTCAGTTGCAAGAAGCTTTATTGTTTTTTGCCTGGT
TTTTAAATTTCTGAGATTTT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSSSQDKANLPANNSRARAKKLVLELQDEICAGLETIDGEGKFLEESWERPEGGGGRSRVLKDGKIFEQGGVNFSEVHGN
ELPPSIISQRPEAKGHSWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDTRHFHSCHKNACD
TIDKDLHKVFKPWCDEYFFLKHRNETRGVGGIFYDYQDGSGLLYKGQNANGKASKIAKELGEYSLNWENLFSLAKACGQA
FLPSYEPIIKKRKNQSFSTKERDFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKPEENSREAL
LTDLFTKPQDWFTDKSLEKRCLTHQALD

Sequences:

>Translated_348_residues
MSSSQDKANLPANNSRARAKKLVLELQDEICAGLETIDGEGKFLEESWERPEGGGGRSRVLKDGKIFEQGGVNFSEVHGN
ELPPSIISQRPEAKGHSWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDTRHFHSCHKNACD
TIDKDLHKVFKPWCDEYFFLKHRNETRGVGGIFYDYQDGSGLLYKGQNANGKASKIAKELGEYSLNWENLFSLAKACGQA
FLPSYEPIIKKRKNQSFSTKERDFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKPEENSREAL
LTDLFTKPQDWFTDKSLEKRCLTHQALD
>Mature_347_residues
SSSQDKANLPANNSRARAKKLVLELQDEICAGLETIDGEGKFLEESWERPEGGGGRSRVLKDGKIFEQGGVNFSEVHGNE
LPPSIISQRPEAKGHSWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDTRHFHSCHKNACDT
IDKDLHKVFKPWCDEYFFLKHRNETRGVGGIFYDYQDGSGLLYKGQNANGKASKIAKELGEYSLNWENLFSLAKACGQAF
LPSYEPIIKKRKNQSFSTKERDFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKPEENSREALL
TDLFTKPQDWFTDKSLEKRCLTHQALD

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=333, Percent_Identity=44.1441441441441, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI1788777, Length=319, Percent_Identity=49.2163009404389, Blast_Score=328, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6320249, Length=355, Percent_Identity=43.6619718309859, Blast_Score=276, Evalue=3e-75,
Organism=Drosophila melanogaster, GI17864388, Length=332, Percent_Identity=46.0843373493976, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_PROM1 (A2C524)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015848.1
- ProteinModelPortal:   A2C524
- SMR:   A2C524
- STRING:   A2C524
- GeneID:   4779705
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_20281
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   VFKPWCD
- ProtClustDB:   PRK05330
- BioCyc:   PMAR167555:NATL1_20281-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 39803; Mature: 39672

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 118-118 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSQDKANLPANNSRARAKKLVLELQDEICAGLETIDGEGKFLEESWERPEGGGGRSRV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHCCCCCCCCCHHHE
LKDGKIFEQGGVNFSEVHGNELPPSIISQRPEAKGHSWFATGTSMVLHPKNPYIPTVHLN
ECCCCEECCCCCCHHHHCCCCCCHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEE
YRYFEAGPVWWFGGGADLTPFYPYLSDTRHFHSCHKNACDTIDKDLHKVFKPWCDEYFFL
EEEEECCCEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KHRNETRGVGGIFYDYQDGSGLLYKGQNANGKASKIAKELGEYSLNWENLFSLAKACGQA
EECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FLPSYEPIIKKRKNQSFSTKERDFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCCCCEEEC
PPLARWEYGYKPEENSREALLTDLFTKPQDWFTDKSLEKRCLTHQALD
CCHHHCCCCCCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSSQDKANLPANNSRARAKKLVLELQDEICAGLETIDGEGKFLEESWERPEGGGGRSRV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHCCCCCCCCCHHHE
LKDGKIFEQGGVNFSEVHGNELPPSIISQRPEAKGHSWFATGTSMVLHPKNPYIPTVHLN
ECCCCEECCCCCCHHHHCCCCCCHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEE
YRYFEAGPVWWFGGGADLTPFYPYLSDTRHFHSCHKNACDTIDKDLHKVFKPWCDEYFFL
EEEEECCCEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KHRNETRGVGGIFYDYQDGSGLLYKGQNANGKASKIAKELGEYSLNWENLFSLAKACGQA
EECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FLPSYEPIIKKRKNQSFSTKERDFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCCCCEEEC
PPLARWEYGYKPEENSREALLTDLFTKPQDWFTDKSLEKRCLTHQALD
CCHHHCCCCCCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA