Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 124026720

GI number: 124026720

Start: 1657930

End: 1659075

Strand: Direct

Name: thiF [H]

Synonym: NATL1_20151

Alternate gene names: 124026720

Gene position: 1657930-1659075 (Clockwise)

Preceding gene: 124026719

Following gene: 124026722

Centisome position: 88.91

GC content: 32.55

Gene sequence:

>1146_bases
ATGGAGCAAAGCCAGAGTAAGGCAAATTTAAGTTCAGAAGAAATTGCCAGGTATGCAAGACATATAAGTCTCCCAGAGAT
AGGTATCAAAGGCCAAGAAAAATTGAAGACAAGCTCAGTTGCTTGCATTGGGACAGGAGGGCTAGGATCTCCACTTTTAA
TTTATCTTGCAGCAGCTGGAATTGGACGTATCGGAATAGTTGATTTTGATGTCGTTGAATACTCAAATTTACAAAGACAA
ATCATTCATACAACACATTCAATAGGTCTATTAAAAACAGATTCGGCCAAACAAGCTATACGCAAAATAAATCCTTCTTG
TCGAGTTGATTTATTCAATCAAAAGCTAACAAGTAGTAATGCTTTGGAAATACTTAAAGCTTATGATGTGATATGTGATT
GTTCAGACAATTTCCCAACGCGTTACCTGATTAATGATGCTTGTCTAATACTTAACAAACCTAATATATATGGTTCAATC
GCAAGATTCGAAGGACAAGTAAGTGTATTTAATTTGAAGGAAGATAGCCCTAACTATAGAGACCTTATCCCCATACCCCC
TCCACAAGAGTTAATTCCATCTTGCTCTGAAGCTGGAGTGATGGGAATTCTTCCAGGAATTATTGGTACAATTCAAGCAG
CAGAAGCTATAAAGATAATAACAAACATTGGTTATCCACTTAACGGTAGGATTCTCATTTTTAATGCATTAAAAATGCAA
TTTAAAGAACTAACTTTGAAATCCAATCCAGAAAATAAAAATATCCATAAATTAATAGATTATAAAAGTTTCTGTTCAGA
AATTTCAGTTAAAGATGAAGTAGAATGTGATATAGAAAGTATTTCAGTTAAAGAATTAAAAGTACTTCTTAGACAATCTT
CAAAAGAAATGTTATTAATAGATGTTCGCAACCAAGATGAATATCATCAATGTTCAATTACAGGTTCATTGCTCATACCT
CTTAACTCTATTGAAAGTGGTAAAGCCATTGATGAAATTAAAATCCTTACCGCAAAAAAAAATCTTTATGTATTTTGTAA
AAGTGGAAAAAGATCATTGCTTGCATTAAAGCATTTAAACAAATTTGGAATTAGAGGTATTAATATTCTTGGAGGTATTG
ATGCGTGGAATAGCGAAAAAAATTAA

Upstream 100 bases:

>100_bases
GAGATTTAAAAGCATGGTGGATTAAAAATAAATTAAAATTCCATAGCGTGAAAATTGAAGTTTTTTCTTTAACGTAAATA
AATCAGCGAAGGTTTGATTA

Downstream 100 bases:

>100_bases
TTAAAACTAATAATCAACTCCCCTTTTTAAATCAACTCCTTTTTCAGCATAGTGCTTGTGACACACCATCTCAGAATGAA
CACTTGCTAAATCAAAATAT

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MEQSQSKANLSSEEIARYARHISLPEIGIKGQEKLKTSSVACIGTGGLGSPLLIYLAAAGIGRIGIVDFDVVEYSNLQRQ
IIHTTHSIGLLKTDSAKQAIRKINPSCRVDLFNQKLTSSNALEILKAYDVICDCSDNFPTRYLINDACLILNKPNIYGSI
ARFEGQVSVFNLKEDSPNYRDLIPIPPPQELIPSCSEAGVMGILPGIIGTIQAAEAIKIITNIGYPLNGRILIFNALKMQ
FKELTLKSNPENKNIHKLIDYKSFCSEISVKDEVECDIESISVKELKVLLRQSSKEMLLIDVRNQDEYHQCSITGSLLIP
LNSIESGKAIDEIKILTAKKNLYVFCKSGKRSLLALKHLNKFGIRGINILGGIDAWNSEKN

Sequences:

>Translated_381_residues
MEQSQSKANLSSEEIARYARHISLPEIGIKGQEKLKTSSVACIGTGGLGSPLLIYLAAAGIGRIGIVDFDVVEYSNLQRQ
IIHTTHSIGLLKTDSAKQAIRKINPSCRVDLFNQKLTSSNALEILKAYDVICDCSDNFPTRYLINDACLILNKPNIYGSI
ARFEGQVSVFNLKEDSPNYRDLIPIPPPQELIPSCSEAGVMGILPGIIGTIQAAEAIKIITNIGYPLNGRILIFNALKMQ
FKELTLKSNPENKNIHKLIDYKSFCSEISVKDEVECDIESISVKELKVLLRQSSKEMLLIDVRNQDEYHQCSITGSLLIP
LNSIESGKAIDEIKILTAKKNLYVFCKSGKRSLLALKHLNKFGIRGINILGGIDAWNSEKN
>Mature_381_residues
MEQSQSKANLSSEEIARYARHISLPEIGIKGQEKLKTSSVACIGTGGLGSPLLIYLAAAGIGRIGIVDFDVVEYSNLQRQ
IIHTTHSIGLLKTDSAKQAIRKINPSCRVDLFNQKLTSSNALEILKAYDVICDCSDNFPTRYLINDACLILNKPNIYGSI
ARFEGQVSVFNLKEDSPNYRDLIPIPPPQELIPSCSEAGVMGILPGIIGTIQAAEAIKIITNIGYPLNGRILIFNALKMQ
FKELTLKSNPENKNIHKLIDYKSFCSEISVKDEVECDIESISVKELKVLLRQSSKEMLLIDVRNQDEYHQCSITGSLLIP
LNSIESGKAIDEIKILTAKKNLYVFCKSGKRSLLALKHLNKFGIRGINILGGIDAWNSEKN

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=407, Percent_Identity=38.0835380835381, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI13376212, Length=268, Percent_Identity=27.9850746268657, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI23510338, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI23510340, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI38327032, Length=256, Percent_Identity=27.734375, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4885649, Length=165, Percent_Identity=25.4545454545455, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI150417996, Length=195, Percent_Identity=28.7179487179487, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=39.5061728395062, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082356, Length=238, Percent_Identity=39.0756302521008, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1789177, Length=111, Percent_Identity=32.4324324324324, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=395, Percent_Identity=33.6708860759494, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI193203301, Length=166, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=401, Percent_Identity=33.9152119700748, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320598, Length=140, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322639, Length=194, Percent_Identity=25.7731958762887, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582879, Length=386, Percent_Identity=37.8238341968912, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24660640, Length=139, Percent_Identity=30.2158273381295, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 42090; Mature: 42090

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSQSKANLSSEEIARYARHISLPEIGIKGQEKLKTSSVACIGTGGLGSPLLIYLAAAG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHCC
IGRIGIVDFDVVEYSNLQRQIIHTTHSIGLLKTDSAKQAIRKINPSCRVDLFNQKLTSSN
CCEEEEEEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCEEEEHHHHCCCCH
ALEILKAYDVICDCSDNFPTRYLINDACLILNKPNIYGSIARFEGQVSVFNLKEDSPNYR
HHHHHHHHHHEEECCCCCCCEEEECCEEEEEECCCCCEEHEEECCEEEEEEECCCCCCCC
DLIPIPPPQELIPSCSEAGVMGILPGIIGTIQAAEAIKIITNIGYPLNGRILIFNALKMQ
CCCCCCCCHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
FKELTLKSNPENKNIHKLIDYKSFCSEISVKDEVECDIESISVKELKVLLRQSSKEMLLI
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCCEEEE
DVRNQDEYHQCSITGSLLIPLNSIESGKAIDEIKILTAKKNLYVFCKSGKRSLLALKHLN
EECCCCCCEEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHHH
KFGIRGINILGGIDAWNSEKN
HHCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MEQSQSKANLSSEEIARYARHISLPEIGIKGQEKLKTSSVACIGTGGLGSPLLIYLAAAG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHCC
IGRIGIVDFDVVEYSNLQRQIIHTTHSIGLLKTDSAKQAIRKINPSCRVDLFNQKLTSSN
CCEEEEEEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCEEEEHHHHCCCCH
ALEILKAYDVICDCSDNFPTRYLINDACLILNKPNIYGSIARFEGQVSVFNLKEDSPNYR
HHHHHHHHHHEEECCCCCCCEEEECCEEEEEECCCCCEEHEEECCEEEEEEECCCCCCCC
DLIPIPPPQELIPSCSEAGVMGILPGIIGTIQAAEAIKIITNIGYPLNGRILIFNALKMQ
CCCCCCCCHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
FKELTLKSNPENKNIHKLIDYKSFCSEISVKDEVECDIESISVKELKVLLRQSSKEMLLI
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCCEEEE
DVRNQDEYHQCSITGSLLIPLNSIESGKAIDEIKILTAKKNLYVFCKSGKRSLLALKHLN
EECCCCCCEEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHHH
KFGIRGINILGGIDAWNSEKN
HHCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]