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The map label for this gene is cobJ [H]

Identifier: 124026661

GI number: 124026661

Start: 1611373

End: 1613154

Strand: Reverse

Name: cobJ [H]

Synonym: NATL1_19561

Alternate gene names: 124026661

Gene position: 1613154-1611373 (Counterclockwise)

Preceding gene: 124026664

Following gene: 124026660

Centisome position: 86.51

GC content: 36.2

Gene sequence:

>1782_bases
TTGCCTTTGCTTGAGAGATTACAAGCATCAAAACATGTAGATCAAATTTTTATATCTGTTGGTTCAACTCTTTCCTTAGA
AAAAAAAATTGATGGTTTAGTTGAATCCTCACCAATTGATTTTCTAAAAGATCAATGGCAGGGCAATAATAAATTAATTT
TTGTAGGTTCAATCGGCGCTGTAGTTAGGCTTATAGCTCCTTTTATTCGATCTAAAGAAAATGATCCAGCAATATTGGTA
ACGGATTCAAAAGCAAAAAATGTTATTACTCTTTTAGGAGGACATAAGCAAGGAGGAGATCGATTTGCTAGTGAGTTAGC
AGCTGCTTTAGAAGCAGAAGTGATTTGCACCTCAGATTCATTCACAGAAAGCAGAATCCCTTTGGATTGTTTTGGTGAAG
GATGGGGTTGGAAAAGAGGAGGGGAGGTTGTCGGTTGGCGAAAGTTAATGATTAGTCAGTCTAAAGAACAAAAAAATATT
GTTTTTCAATCCAAAGGGTCAAAATTGTGGCAGCAATTAAAGGGTTGTTCCAATTTTTCTTTTTTAGAAAAAAATGATCC
AATTTCTTTCGAAAATATCGATCTTTATATAGGTCAAGAAAATAGGAATAAATGTTCATGGCATCCGCCAACAATAATCA
TTGGCATTGGCTGTGAGAGAAATACAGATGAAAAGGTCATTCAGAGAGCAATTAATAATTCTTTGAATCAAAATGGTTTA
TCACTCCTTTCAATCTCTGGTTTAGCAACTATCGATAGAAAAAATGATGAAATAGGATTATTGAGTTTGTCAAAAAAACA
TGAATGGCCAATTTATTTTTTTAGTGCCTTTGAACTTTCAAAAGTCAACGTGCCTACTCCTTCAAATGTAGTGTTGAATG
AAATGGGAACTCCTTCAGTCGCTGAAGCTGCAGCACTTTTGGCAGGAGATCATCGTGGAAGGTTAATACAAAATAAACAA
ATCTATTATTCCAAGGGAGAGGAATCTGGTGCCGTAACAATTGCATTGGCTGAGGTTGAAAACCCTTTTGCCCCTCATAA
AGGAGAGTTGCATTTGATTGGTAGTGGCCCTGGAGACTTGAAAATGCTTACTTCTGACTCACGCCAAGCTTTAACTAGAT
GTGCTGTTTGGATCGGTTATGGTCCTTACTTAAATTATTTGGAATCAATTCGAGGTCATAATCAGGTTCGAATTGATTCC
AAATTAACTTTTGAAAAAGATCGTTGTCAGCATGCTCTTGATCTTGCAAAACAGGGAGTAAAGGTTGGTCTTATTTCTTC
TGGCGACAGTGGAATTTATGGCATGGCAGGATTGGCACTTGATCTTTGGCTAAATGAAAGGCTTGATTCCAGACCTTTAT
TTGAAGTTCATCCAGGCATTAGCTCTTTTCAAATGGCTGCTGCAAAACTAGGAGCTCCTTTTATGCATGATTTTTGTTCT
ATTTCTTTGAGTGATCTTTTGACTCCATGGAATCAAATTGAGAAAAGAATAAAAAGTGCAGCTATAGGTGACTTTGTTCT
TGCAATATTTAACCCGAAATCAATAAAACGTGATTGGCAGTTAAAAAAGACTGTTGATTTATTACTTGAATGTCGCAACC
CAAGTACTCCTGTTGCTATTGCTCGGCAGCTTGGGAGGCCAGAGGAAAGTATAGAAATACATACTCTGGAAACATTGCCA
TTTGATCGGGTAGATATGCTTACGATTTTGGTTATTGGTAATAGTCAAAGTGTGATCAAAAACAATAAATTTTTAACGCC
AAGAGGTTATTTTTCTAATTAA

Upstream 100 bases:

>100_bases
ACCTTATATAGATAAATAAAAATTTATTTTATCTCTCGTTCTGGAAAAAACATTAGTTAAAACCCCAAAAAGGATTGCAT
TTGGATTTTCTTTAAGCTCT

Downstream 100 bases:

>100_bases
TTTTTTGTTTACTTCTTCTAACTAGAAAATCGAATACTTTGTTTTAGGCTTATTTTTGCTTGTCAAGTTGAGAAGACGTT
AAAAAATCAAGTCAATCTGG

Product: bifunctional cbiH protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MPLLERLQASKHVDQIFISVGSTLSLEKKIDGLVESSPIDFLKDQWQGNNKLIFVGSIGAVVRLIAPFIRSKENDPAILV
TDSKAKNVITLLGGHKQGGDRFASELAAALEAEVICTSDSFTESRIPLDCFGEGWGWKRGGEVVGWRKLMISQSKEQKNI
VFQSKGSKLWQQLKGCSNFSFLEKNDPISFENIDLYIGQENRNKCSWHPPTIIIGIGCERNTDEKVIQRAINNSLNQNGL
SLLSISGLATIDRKNDEIGLLSLSKKHEWPIYFFSAFELSKVNVPTPSNVVLNEMGTPSVAEAAALLAGDHRGRLIQNKQ
IYYSKGEESGAVTIALAEVENPFAPHKGELHLIGSGPGDLKMLTSDSRQALTRCAVWIGYGPYLNYLESIRGHNQVRIDS
KLTFEKDRCQHALDLAKQGVKVGLISSGDSGIYGMAGLALDLWLNERLDSRPLFEVHPGISSFQMAAAKLGAPFMHDFCS
ISLSDLLTPWNQIEKRIKSAAIGDFVLAIFNPKSIKRDWQLKKTVDLLLECRNPSTPVAIARQLGRPEESIEIHTLETLP
FDRVDMLTILVIGNSQSVIKNNKFLTPRGYFSN

Sequences:

>Translated_593_residues
MPLLERLQASKHVDQIFISVGSTLSLEKKIDGLVESSPIDFLKDQWQGNNKLIFVGSIGAVVRLIAPFIRSKENDPAILV
TDSKAKNVITLLGGHKQGGDRFASELAAALEAEVICTSDSFTESRIPLDCFGEGWGWKRGGEVVGWRKLMISQSKEQKNI
VFQSKGSKLWQQLKGCSNFSFLEKNDPISFENIDLYIGQENRNKCSWHPPTIIIGIGCERNTDEKVIQRAINNSLNQNGL
SLLSISGLATIDRKNDEIGLLSLSKKHEWPIYFFSAFELSKVNVPTPSNVVLNEMGTPSVAEAAALLAGDHRGRLIQNKQ
IYYSKGEESGAVTIALAEVENPFAPHKGELHLIGSGPGDLKMLTSDSRQALTRCAVWIGYGPYLNYLESIRGHNQVRIDS
KLTFEKDRCQHALDLAKQGVKVGLISSGDSGIYGMAGLALDLWLNERLDSRPLFEVHPGISSFQMAAAKLGAPFMHDFCS
ISLSDLLTPWNQIEKRIKSAAIGDFVLAIFNPKSIKRDWQLKKTVDLLLECRNPSTPVAIARQLGRPEESIEIHTLETLP
FDRVDMLTILVIGNSQSVIKNNKFLTPRGYFSN
>Mature_592_residues
PLLERLQASKHVDQIFISVGSTLSLEKKIDGLVESSPIDFLKDQWQGNNKLIFVGSIGAVVRLIAPFIRSKENDPAILVT
DSKAKNVITLLGGHKQGGDRFASELAAALEAEVICTSDSFTESRIPLDCFGEGWGWKRGGEVVGWRKLMISQSKEQKNIV
FQSKGSKLWQQLKGCSNFSFLEKNDPISFENIDLYIGQENRNKCSWHPPTIIIGIGCERNTDEKVIQRAINNSLNQNGLS
LLSISGLATIDRKNDEIGLLSLSKKHEWPIYFFSAFELSKVNVPTPSNVVLNEMGTPSVAEAAALLAGDHRGRLIQNKQI
YYSKGEESGAVTIALAEVENPFAPHKGELHLIGSGPGDLKMLTSDSRQALTRCAVWIGYGPYLNYLESIRGHNQVRIDSK
LTFEKDRCQHALDLAKQGVKVGLISSGDSGIYGMAGLALDLWLNERLDSRPLFEVHPGISSFQMAAAKLGAPFMHDFCSI
SLSDLLTPWNQIEKRIKSAAIGDFVLAIFNPKSIKRDWQLKKTVDLLLECRNPSTPVAIARQLGRPEESIEIHTLETLPF
DRVDMLTILVIGNSQSVIKNNKFLTPRGYFSN

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 65644; Mature: 65513

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLERLQASKHVDQIFISVGSTLSLEKKIDGLVESSPIDFLKDQWQGNNKLIFVGSIGA
CCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECHHH
VVRLIAPFIRSKENDPAILVTDSKAKNVITLLGGHKQGGDRFASELAAALEAEVICTSDS
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCC
FTESRIPLDCFGEGWGWKRGGEVVGWRKLMISQSKEQKNIVFQSKGSKLWQQLKGCSNFS
CCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCCC
FLEKNDPISFENIDLYIGQENRNKCSWHPPTIIIGIGCERNTDEKVIQRAINNSLNQNGL
EECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
SLLSISGLATIDRKNDEIGLLSLSKKHEWPIYFFSAFELSKVNVPTPSNVVLNEMGTPSV
EEEEECCEEEECCCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCCCHH
AEAAALLAGDHRGRLIQNKQIYYSKGEESGAVTIALAEVENPFAPHKGELHLIGSGPGDL
HHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCE
KMLTSDSRQALTRCAVWIGYGPYLNYLESIRGHNQVRIDSKLTFEKDRCQHALDLAKQGV
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
KVGLISSGDSGIYGMAGLALDLWLNERLDSRPLFEVHPGISSFQMAAAKLGAPFMHDFCS
EEEEEECCCCCCEEHHHHHEEEHHHCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHC
ISLSDLLTPWNQIEKRIKSAAIGDFVLAIFNPKSIKRDWQLKKTVDLLLECRNPSTPVAI
CCHHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHCCCHHHHHHHHHHHCCCCCCCHHHH
ARQLGRPEESIEIHTLETLPFDRVDMLTILVIGNSQSVIKNNKFLTPRGYFSN
HHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHCCCCEECCCCCCCC
>Mature Secondary Structure 
PLLERLQASKHVDQIFISVGSTLSLEKKIDGLVESSPIDFLKDQWQGNNKLIFVGSIGA
CHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECHHH
VVRLIAPFIRSKENDPAILVTDSKAKNVITLLGGHKQGGDRFASELAAALEAEVICTSDS
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCC
FTESRIPLDCFGEGWGWKRGGEVVGWRKLMISQSKEQKNIVFQSKGSKLWQQLKGCSNFS
CCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCCC
FLEKNDPISFENIDLYIGQENRNKCSWHPPTIIIGIGCERNTDEKVIQRAINNSLNQNGL
EECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
SLLSISGLATIDRKNDEIGLLSLSKKHEWPIYFFSAFELSKVNVPTPSNVVLNEMGTPSV
EEEEECCEEEECCCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCCCHH
AEAAALLAGDHRGRLIQNKQIYYSKGEESGAVTIALAEVENPFAPHKGELHLIGSGPGDL
HHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCE
KMLTSDSRQALTRCAVWIGYGPYLNYLESIRGHNQVRIDSKLTFEKDRCQHALDLAKQGV
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
KVGLISSGDSGIYGMAGLALDLWLNERLDSRPLFEVHPGISSFQMAAAKLGAPFMHDFCS
EEEEEECCCCCCEEHHHHHEEEHHHCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHC
ISLSDLLTPWNQIEKRIKSAAIGDFVLAIFNPKSIKRDWQLKKTVDLLLECRNPSTPVAI
CCHHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHCCCHHHHHHHHHHHCCCCCCCHHHH
ARQLGRPEESIEIHTLETLPFDRVDMLTILVIGNSQSVIKNNKFLTPRGYFSN
HHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]