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The map label for this gene is lipA1 [H]

Identifier: 124026660

GI number: 124026660

Start: 1610366

End: 1611235

Strand: Reverse

Name: lipA1 [H]

Synonym: NATL1_19551

Alternate gene names: 124026660

Gene position: 1611235-1610366 (Counterclockwise)

Preceding gene: 124026661

Following gene: 161407968

Centisome position: 86.41

GC content: 36.9

Gene sequence:

>870_bases
TTGCTTAAACCAGACTGGCTTCGTGTTAAAGCACCACAGCAAGAGAGGATTGGTAATGTTGCTGATCTATTAGTTGATTT
AAAACTCAATACAGTTTGTCAGGAAGCAAGTTGTCCAAATATTGGAGAGTGTTTTGCAGGAGGTACTGCTACTTTCTTAA
TAATGGGACCTGCTTGCACGAGAAAATGTCCTTATTGCGATATTTCATTTGATAATTCAAAAAGGCTTTTAGACCCATCA
GAACCTGATCGTCTAGGTGAAGCTGTTTACAGAATGGGCTTAACGCATGTCGTAATAACTTCCGTTAATCGTGATGATTT
AGAAGATGGAGGCGCTAGTCAATTTTTGAAATGTATTAAGGCTGTGCAAAGTAAATCTCCTTTTACTTCTATTGAAGTCT
TAATCCCAGATCTTTGCGGAAATTGGGATGCTTTGAAAGTTATCTTGGATGCTGCGCCAAATGTCCTTAATCACAATATC
GAGACAGTTCCGAGGCTATATCCAAGAGTTAGACCTCAAGGGAAATATGAAAGGTCTTTAGAATTATTAAAAAGAGTTCG
GGAAGGTTGGCCAAGAGTTTATACAAAATCAGGACTAATGGCTGGATTAGGGGAGACAGATGATGAGATTTTGAGTGTTC
TAAGTGATCTCCATTTAAATAAAGTTGATATAGTAACTATTGGGCAATATTTATCACCAGGACCAAAACATTTACCTGTT
AATAGATTTGTTTCCCCTTCGCAATTTGATAGTTATCGTGTTGAGGGAGAAAATAAATTAGGATTTTTACAGGTCATTAG
TTCACCATTAACTAGAAGTAGTTATCACGCTGGAGAAGTTAAAAAATTAATGATGTCTCATCCAAGATGA

Upstream 100 bases:

>100_bases
TTTGTTTTAGGCTTATTTTTGCTTGTCAAGTTGAGAAGACGTTAAAAAATCAAGTCAATCTGGAAATTCTTTCTAAAGTT
AGTTTGTTATTAAAAGAGCT

Downstream 100 bases:

>100_bases
TTTGAAAACTTTGGTTGACTTCCATCAATTGATATCCATTCTTGAAGATCCCATGAAACTAATTCATACTTAATCATTGG
ATCATTTTCTACAATTTTTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLKPDWLRVKAPQQERIGNVADLLVDLKLNTVCQEASCPNIGECFAGGTATFLIMGPACTRKCPYCDISFDNSKRLLDPS
EPDRLGEAVYRMGLTHVVITSVNRDDLEDGGASQFLKCIKAVQSKSPFTSIEVLIPDLCGNWDALKVILDAAPNVLNHNI
ETVPRLYPRVRPQGKYERSLELLKRVREGWPRVYTKSGLMAGLGETDDEILSVLSDLHLNKVDIVTIGQYLSPGPKHLPV
NRFVSPSQFDSYRVEGENKLGFLQVISSPLTRSSYHAGEVKKLMMSHPR

Sequences:

>Translated_289_residues
MLKPDWLRVKAPQQERIGNVADLLVDLKLNTVCQEASCPNIGECFAGGTATFLIMGPACTRKCPYCDISFDNSKRLLDPS
EPDRLGEAVYRMGLTHVVITSVNRDDLEDGGASQFLKCIKAVQSKSPFTSIEVLIPDLCGNWDALKVILDAAPNVLNHNI
ETVPRLYPRVRPQGKYERSLELLKRVREGWPRVYTKSGLMAGLGETDDEILSVLSDLHLNKVDIVTIGQYLSPGPKHLPV
NRFVSPSQFDSYRVEGENKLGFLQVISSPLTRSSYHAGEVKKLMMSHPR
>Mature_289_residues
MLKPDWLRVKAPQQERIGNVADLLVDLKLNTVCQEASCPNIGECFAGGTATFLIMGPACTRKCPYCDISFDNSKRLLDPS
EPDRLGEAVYRMGLTHVVITSVNRDDLEDGGASQFLKCIKAVQSKSPFTSIEVLIPDLCGNWDALKVILDAAPNVLNHNI
ETVPRLYPRVRPQGKYERSLELLKRVREGWPRVYTKSGLMAGLGETDDEILSVLSDLHLNKVDIVTIGQYLSPGPKHLPV
NRFVSPSQFDSYRVEGENKLGFLQVISSPLTRSSYHAGEVKKLMMSHPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=39.3617021276596, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=38.9344262295082, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=48.014440433213, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=43.859649122807, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=39.9293286219081, Blast_Score=225, Evalue=8e-60,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=41.8439716312057, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32163; Mature: 32163

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPDWLRVKAPQQERIGNVADLLVDLKLNTVCQEASCPNIGECFAGGTATFLIMGPACT
CCCCCCEEECCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHC
RKCPYCDISFDNSKRLLDPSEPDRLGEAVYRMGLTHVVITSVNRDDLEDGGASQFLKCIK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHCCCCCCHHHHHHHHH
AVQSKSPFTSIEVLIPDLCGNWDALKVILDAAPNVLNHNIETVPRLYPRVRPQGKYERSL
HHHCCCCCCCCEEEEHHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCHHHHH
ELLKRVREGWPRVYTKSGLMAGLGETDDEILSVLSDLHLNKVDIVTIGQYLSPGPKHLPV
HHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCC
NRFVSPSQFDSYRVEGENKLGFLQVISSPLTRSSYHAGEVKKLMMSHPR
CCCCCCCCCCCEEECCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLKPDWLRVKAPQQERIGNVADLLVDLKLNTVCQEASCPNIGECFAGGTATFLIMGPACT
CCCCCCEEECCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHC
RKCPYCDISFDNSKRLLDPSEPDRLGEAVYRMGLTHVVITSVNRDDLEDGGASQFLKCIK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHCCCCCCHHHHHHHHH
AVQSKSPFTSIEVLIPDLCGNWDALKVILDAAPNVLNHNIETVPRLYPRVRPQGKYERSL
HHHCCCCCCCCEEEEHHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCHHHHH
ELLKRVREGWPRVYTKSGLMAGLGETDDEILSVLSDLHLNKVDIVTIGQYLSPGPKHLPV
HHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCC
NRFVSPSQFDSYRVEGENKLGFLQVISSPLTRSSYHAGEVKKLMMSHPR
CCCCCCCCCCCEEECCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]