Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 124026579

GI number: 124026579

Start: 1533091

End: 1533837

Strand: Direct

Name: gidB

Synonym: NATL1_18741

Alternate gene names: 124026579

Gene position: 1533091-1533837 (Clockwise)

Preceding gene: 124026577

Following gene: 124026594

Centisome position: 82.22

GC content: 36.55

Gene sequence:

>747_bases
ATGTCTACTGATAAAAAAAACAGAACAGTAAGTCATCTCATGATATGGAATGAGCTTAAATGGGCGCCAAGTGAAAAACA
ATTAGCTCAATTCATCCACTTGCAAGAATTACTGAAAGAATGGAATAAGAAGATCAACCTTACTCGTTTAGTCGATGGAG
ATGATTTCTGGACTGCACAAGTATGCGATAGCTTGTTGCCACTATATGAAGAACTTCAACATCCGGAAGTTTCTCATAAA
TATATTGATATTGGTTCTGGCTGTGGATTTCCTGGTATAGCCATAGCTATAGCAATGCCAAATTCAGATATTACTCTTTT
AGATTCTTCCAGTAAAAAAACAACTTTTCTGAAAGAAGTTTCTAAAGAGATTGGATTGGATTCTCGGATAAAAGTCGTAA
CCGAACGTGCTGAGGAAGCAGGAAGGAATCCAATCTTTCGAAGTAATTTTGACTATGCCATTGCAAGAGCAGTTGCTTCT
GCAAATGTAGTAGCAGAATATCTCGTGCCTTTTTTAAATTCAACAGGTCAAGCGCTCATATTCAAAGGAAGCTGGAGTGA
AGCAGAGCAACAAATACTAAAAAAAGCTTTAGCTGAACTGAATGCAGAGATCCAAAGAACACATGAATTCATACTCCCTA
ACAATCGCGGTATTAGAAATATTATTAGGATTAGTTCTATTAATAAATGTCCCCATCAATACCCAAGATCAATTGGTAAA
CCCAAAAAACAGCCTTTAGGCTACTAA

Upstream 100 bases:

>100_bases
TAACAATTGGAAGAAGGGGCCAACTAAAGAAAATATGATCTAATAATGTATAGATTTTATCTAAATATCTCAGGACTGAC
TATTTCAAAAACAATTAACA

Downstream 100 bases:

>100_bases
ATCCCCCATAATCTATCTCTTCTTTCCCCTCCCAATCTATCTTTCAATTTTCTAAGTACAGAAGGTATTTCAGAGCTCCA
AGGGCTATTAATCAAAACTT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSTDKKNRTVSHLMIWNELKWAPSEKQLAQFIHLQELLKEWNKKINLTRLVDGDDFWTAQVCDSLLPLYEELQHPEVSHK
YIDIGSGCGFPGIAIAIAMPNSDITLLDSSSKKTTFLKEVSKEIGLDSRIKVVTERAEEAGRNPIFRSNFDYAIARAVAS
ANVVAEYLVPFLNSTGQALIFKGSWSEAEQQILKKALAELNAEIQRTHEFILPNNRGIRNIIRISSINKCPHQYPRSIGK
PKKQPLGY

Sequences:

>Translated_248_residues
MSTDKKNRTVSHLMIWNELKWAPSEKQLAQFIHLQELLKEWNKKINLTRLVDGDDFWTAQVCDSLLPLYEELQHPEVSHK
YIDIGSGCGFPGIAIAIAMPNSDITLLDSSSKKTTFLKEVSKEIGLDSRIKVVTERAEEAGRNPIFRSNFDYAIARAVAS
ANVVAEYLVPFLNSTGQALIFKGSWSEAEQQILKKALAELNAEIQRTHEFILPNNRGIRNIIRISSINKCPHQYPRSIGK
PKKQPLGY
>Mature_247_residues
STDKKNRTVSHLMIWNELKWAPSEKQLAQFIHLQELLKEWNKKINLTRLVDGDDFWTAQVCDSLLPLYEELQHPEVSHKY
IDIGSGCGFPGIAIAIAMPNSDITLLDSSSKKTTFLKEVSKEIGLDSRIKVVTERAEEAGRNPIFRSNFDYAIARAVASA
NVVAEYLVPFLNSTGQALIFKGSWSEAEQQILKKALAELNAEIQRTHEFILPNNRGIRNIIRISSINKCPHQYPRSIGKP
KKQPLGY

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=170, Percent_Identity=32.9411764705882, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_PROM1 (A2C4M0)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015694.1
- ProteinModelPortal:   A2C4M0
- SMR:   A2C4M0
- STRING:   A2C4M0
- GeneID:   4780199
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_18741
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- ProtClustDB:   PRK00107
- BioCyc:   PMAR167555:NATL1_18741-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 28061; Mature: 27929

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDKKNRTVSHLMIWNELKWAPSEKQLAQFIHLQELLKEWNKKINLTRLVDGDDFWTAQ
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
VCDSLLPLYEELQHPEVSHKYIDIGSGCGFPGIAIAIAMPNSDITLLDSSSKKTTFLKEV
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHH
SKEIGLDSRIKVVTERAEEAGRNPIFRSNFDYAIARAVASANVVAEYLVPFLNSTGQALI
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FKGSWSEAEQQILKKALAELNAEIQRTHEFILPNNRGIRNIIRISSINKCPHQYPRSIGK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
PKKQPLGY
CCCCCCCC
>Mature Secondary Structure 
STDKKNRTVSHLMIWNELKWAPSEKQLAQFIHLQELLKEWNKKINLTRLVDGDDFWTAQ
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
VCDSLLPLYEELQHPEVSHKYIDIGSGCGFPGIAIAIAMPNSDITLLDSSSKKTTFLKEV
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHH
SKEIGLDSRIKVVTERAEEAGRNPIFRSNFDYAIARAVASANVVAEYLVPFLNSTGQALI
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FKGSWSEAEQQILKKALAELNAEIQRTHEFILPNNRGIRNIIRISSINKCPHQYPRSIGK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
PKKQPLGY
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA