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The map label for this gene is purB [H]

Identifier: 124026570

GI number: 124026570

Start: 1522143

End: 1523438

Strand: Direct

Name: purB [H]

Synonym: NATL1_18651

Alternate gene names: 124026570

Gene position: 1522143-1523438 (Clockwise)

Preceding gene: 124026569

Following gene: 124026571

Centisome position: 81.63

GC content: 38.27

Gene sequence:

>1296_bases
TTGATTGAGCGTTACACAAACCCAGAGATGGGAAATATTTGGTCTGATCAAGCCAAATACCAAACATGGCTTGATGTTGA
AATTGCCGCATGTGAGGCTAATTGCAAATTAGGGAAAATCCCTCAAAGTGCAATGGAAACAATTCGAACAAAGGCAAGAT
TCAAGCCAGAACGCATACTCGAAATAGAGGAGGAAGTTCGCCATGACGTAATTGCCTTTCTAACAAATGTAAATGAATAT
GTTGGGGATGCTGGCCGTTACATTCACGTTGGAATGACCAGTAGCGATGTCCTTGATACTGGTCTTGCACTGCAATTAAA
GTCATCCGTCAAACTTTTAAGAAAAGAGCTTTTATTACTTGAAGAAGCTATTAGAGATTTAGCAAGTCAGCATAAAAAAA
CCGTAATGATTGGACGTTCTCATGCCATTCATGGAGAACCTATTACCTTTGGATTCAAGTTGGCGGGATGGCTAGCTGAA
ACTCTCAGGAACAAAGATAGGCTAAACAGTCTTGAGAAAGATATTTCTGTTGGTCAAATCAGCGGAGCTATGGGCACTTA
TGCCAATACTGATCCAGAAATAGAAAAAATAACTTGCGAACTTTTGGAGCTTGATTGTGACACTGCTAGCACTCAAGTTA
TCTCAAGAGATAGGCATGCTAATTATGTGCAGATTCTTGCTTTGATTGGATCTTCACTAGATCGTTTTTCTACAGAAATT
AGAAACCTTCAAAGAACTGATGTTCTTGAAGTAGAGGAAAACTTTGCTAAAGGCCAAAAAGGAAGCTCTGCAATGCCTCA
TAAAAGAAATCCTATACGTAGTGAACGGGTAAGTGGGCTTTCCAGGGTTTTAAGAAGTTATGTAGTTGCAGCTCTTGAAA
ATGTAGCCCTATGGCACGAAAGAGATATAAGCCACAGCTCCAATGAAAGATTAATGCTGCCAGACACATCTATTACTCTT
CATTTCATGCTCACAGAAATGACCGCAATAATTAAAGGTCTTGGAGTATATCCAAATAATATGCTGAAAAATTTGAACAT
TTATGGAGGAGTAGTGTTTAGTCAAAGAGTACTTTTGGCTTTAGTTGAGAATGGAATGAGTAGAGAAGATTCTTATAGAT
TAGTTCAAAAAAATGCTCATTCAGCCTGGAATCAACCCGAAGGAAATTTCAAAAAGAACCTTGAGAATGACCCAGAGGTA
ATGAATAGTCTCTCTACTGAAAAACTCTCTGATTGCTTCTCAACCGAATTACATCAATCAAATTTGAGAGTTATTTGGGA
AAGACTTGGCATATAA

Upstream 100 bases:

>100_bases
CTTGGGGAAGATATGTCCATTGGGAAGTTGAACACAATAGACTCAAGTCAGCTTGACCTTGAGGATCTAAATTAATAGCA
ACCTTTAACTTTGTAGCACT

Downstream 100 bases:

>100_bases
CTTTAAAATCTGAATCAATTAATATCAGTGAATACACTCTAAAAAGGTAAAAGAACTATATTTAATCAAAATCTATATTT
ATAATTTTCTATTCCTTAGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTNPEMGNIWSDQAKYQTWLDVEIAACEANCKLGKIPQSAMETIRTKARFKPERILEIEEEVRHDVIAFLTNVNEY
VGDAGRYIHVGMTSSDVLDTGLALQLKSSVKLLRKELLLLEEAIRDLASQHKKTVMIGRSHAIHGEPITFGFKLAGWLAE
TLRNKDRLNSLEKDISVGQISGAMGTYANTDPEIEKITCELLELDCDTASTQVISRDRHANYVQILALIGSSLDRFSTEI
RNLQRTDVLEVEENFAKGQKGSSAMPHKRNPIRSERVSGLSRVLRSYVVAALENVALWHERDISHSSNERLMLPDTSITL
HFMLTEMTAIIKGLGVYPNNMLKNLNIYGGVVFSQRVLLALVENGMSREDSYRLVQKNAHSAWNQPEGNFKKNLENDPEV
MNSLSTEKLSDCFSTELHQSNLRVIWERLGI

Sequences:

>Translated_431_residues
MIERYTNPEMGNIWSDQAKYQTWLDVEIAACEANCKLGKIPQSAMETIRTKARFKPERILEIEEEVRHDVIAFLTNVNEY
VGDAGRYIHVGMTSSDVLDTGLALQLKSSVKLLRKELLLLEEAIRDLASQHKKTVMIGRSHAIHGEPITFGFKLAGWLAE
TLRNKDRLNSLEKDISVGQISGAMGTYANTDPEIEKITCELLELDCDTASTQVISRDRHANYVQILALIGSSLDRFSTEI
RNLQRTDVLEVEENFAKGQKGSSAMPHKRNPIRSERVSGLSRVLRSYVVAALENVALWHERDISHSSNERLMLPDTSITL
HFMLTEMTAIIKGLGVYPNNMLKNLNIYGGVVFSQRVLLALVENGMSREDSYRLVQKNAHSAWNQPEGNFKKNLENDPEV
MNSLSTEKLSDCFSTELHQSNLRVIWERLGI
>Mature_431_residues
MIERYTNPEMGNIWSDQAKYQTWLDVEIAACEANCKLGKIPQSAMETIRTKARFKPERILEIEEEVRHDVIAFLTNVNEY
VGDAGRYIHVGMTSSDVLDTGLALQLKSSVKLLRKELLLLEEAIRDLASQHKKTVMIGRSHAIHGEPITFGFKLAGWLAE
TLRNKDRLNSLEKDISVGQISGAMGTYANTDPEIEKITCELLELDCDTASTQVISRDRHANYVQILALIGSSLDRFSTEI
RNLQRTDVLEVEENFAKGQKGSSAMPHKRNPIRSERVSGLSRVLRSYVVAALENVALWHERDISHSSNERLMLPDTSITL
HFMLTEMTAIIKGLGVYPNNMLKNLNIYGGVVFSQRVLLALVENGMSREDSYRLVQKNAHSAWNQPEGNFKKNLENDPEV
MNSLSTEKLSDCFSTELHQSNLRVIWERLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=407, Percent_Identity=27.5184275184275, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=27.6041666666667, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1787376, Length=331, Percent_Identity=26.5861027190332, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790398, Length=246, Percent_Identity=25.609756097561, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=27.5252525252525, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564234, Length=341, Percent_Identity=28.1524926686217, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=27.2727272727273, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24647570, Length=410, Percent_Identity=27.0731707317073, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48700; Mature: 48700

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTNPEMGNIWSDQAKYQTWLDVEIAACEANCKLGKIPQSAMETIRTKARFKPERIL
CCCCCCCCCCCCCCCCCHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHH
EIEEEVRHDVIAFLTNVNEYVGDAGRYIHVGMTSSDVLDTGLALQLKSSVKLLRKELLLL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHH
EEAIRDLASQHKKTVMIGRSHAIHGEPITFGFKLAGWLAETLRNKDRLNSLEKDISVGQI
HHHHHHHHHHHHCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHCCHHHH
SGAMGTYANTDPEIEKITCELLELDCDTASTQVISRDRHANYVQILALIGSSLDRFSTEI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEENFAKGQKGSSAMPHKRNPIRSERVSGLSRVLRSYVVAALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSNERLMLPDTSITLHFMLTEMTAIIKGLGVYPNNMLKNLNIYGGVVFSQRVLLA
CCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHH
LVENGMSREDSYRLVQKNAHSAWNQPEGNFKKNLENDPEVMNSLSTEKLSDCFSTELHQS
HHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NLRVIWERLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTNPEMGNIWSDQAKYQTWLDVEIAACEANCKLGKIPQSAMETIRTKARFKPERIL
CCCCCCCCCCCCCCCCCHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHH
EIEEEVRHDVIAFLTNVNEYVGDAGRYIHVGMTSSDVLDTGLALQLKSSVKLLRKELLLL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHH
EEAIRDLASQHKKTVMIGRSHAIHGEPITFGFKLAGWLAETLRNKDRLNSLEKDISVGQI
HHHHHHHHHHHHCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHCCHHHH
SGAMGTYANTDPEIEKITCELLELDCDTASTQVISRDRHANYVQILALIGSSLDRFSTEI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEENFAKGQKGSSAMPHKRNPIRSERVSGLSRVLRSYVVAALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSNERLMLPDTSITLHFMLTEMTAIIKGLGVYPNNMLKNLNIYGGVVFSQRVLLA
CCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHH
LVENGMSREDSYRLVQKNAHSAWNQPEGNFKKNLENDPEVMNSLSTEKLSDCFSTELHQS
HHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NLRVIWERLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]