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The map label for this gene is pyrF

Identifier: 124026460

GI number: 124026460

Start: 1436294

End: 1437019

Strand: Reverse

Name: pyrF

Synonym: NATL1_17551

Alternate gene names: 124026460

Gene position: 1437019-1436294 (Counterclockwise)

Preceding gene: 124026461

Following gene: 124026452

Centisome position: 77.06

GC content: 34.57

Gene sequence:

>726_bases
ATGAAAAATATTGATAATCCAAGTGAAAAAATAATTATTGCTTTAGATGGAATGGATAAAGATGATGTTGTCAATTTATT
GAAAAAAATACCTGAAATTGTCTGGGTTAAAGTTGGACTCGAGTTGTTTGTTAGTGAAGGACCAGACGTATTATCAATGT
TAAGAGAAAAGGGTAAAAAAATATTTTTAGATCTTAAGTTTCATGATATTCCTACAACAGTTGCTAGAGCATGTTTCGCA
GCATCACAAACTGGTGCTGAATTTATTTCTTTGCATACTTGCGCTGGTATGAAGGCTCTAAAGATGGCCAATGAAGCGGC
AAATGAAGGAGCCTCTAAAGTTAATCTAATTCCGCCAAAACTTTTGGGAATTACAATATTGACGAGTTGGACTCGCGAAA
GTTTTTGTAATGATCTTCTAATTAATCAATCAATAGATCAACGTGTTAAGCACCTGGCAGAGATTGCGTCGAATTCTGGT
TTAGGAGGATGTGTCTGTAGTCCTAAAGAGGTTCAATTTCTTCGTGAATCTTATCCTGAGACTTTTGAGTTGATAACTCC
AGGGATAAGATCATTAGGTTCAAATGTTAATGATCAATCTAGGGTTTCTGATGCATCCGAAGCTATTAAGATGGGAGCAT
CAAAGTTAGTTATCGGAAGAGCAATTACCCAATCAAATGATTCTGCTTATATGTTTAAGAGTTTTTGCGATAAAATTAGT
ATTTAA

Upstream 100 bases:

>100_bases
GGATCCTAATTTAGAGTTTCACACTCCAGATGATCTCATAGGAAAAATATTACAAGTAGGAAAGAAGAAATTTCTTCGTG
TTTCAACTTGATAGATTGTT

Downstream 100 bases:

>100_bases
TTTCTTAGGGCTGTCCGATTCTTGGTTCTTTACCCCTAATTAGCCTAACTATATTCTCTCTATGTCTCCAAATCACTAAT
AACATAGCTAATAGTGAAAC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKNIDNPSEKIIIALDGMDKDDVVNLLKKIPEIVWVKVGLELFVSEGPDVLSMLREKGKKIFLDLKFHDIPTTVARACFA
ASQTGAEFISLHTCAGMKALKMANEAANEGASKVNLIPPKLLGITILTSWTRESFCNDLLINQSIDQRVKHLAEIASNSG
LGGCVCSPKEVQFLRESYPETFELITPGIRSLGSNVNDQSRVSDASEAIKMGASKLVIGRAITQSNDSAYMFKSFCDKIS
I

Sequences:

>Translated_241_residues
MKNIDNPSEKIIIALDGMDKDDVVNLLKKIPEIVWVKVGLELFVSEGPDVLSMLREKGKKIFLDLKFHDIPTTVARACFA
ASQTGAEFISLHTCAGMKALKMANEAANEGASKVNLIPPKLLGITILTSWTRESFCNDLLINQSIDQRVKHLAEIASNSG
LGGCVCSPKEVQFLRESYPETFELITPGIRSLGSNVNDQSRVSDASEAIKMGASKLVIGRAITQSNDSAYMFKSFCDKIS
I
>Mature_241_residues
MKNIDNPSEKIIIALDGMDKDDVVNLLKKIPEIVWVKVGLELFVSEGPDVLSMLREKGKKIFLDLKFHDIPTTVARACFA
ASQTGAEFISLHTCAGMKALKMANEAANEGASKVNLIPPKLLGITILTSWTRESFCNDLLINQSIDQRVKHLAEIASNSG
LGGCVCSPKEVQFLRESYPETFELITPGIRSLGSNVNDQSRVSDASEAIKMGASKLVIGRAITQSNDSAYMFKSFCDKIS
I

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=36.0869565217391, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PROM1 (A2C4A1)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015575.1
- ProteinModelPortal:   A2C4A1
- SMR:   A2C4A1
- STRING:   A2C4A1
- GeneID:   4779729
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_17551
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK938844
- BioCyc:   PMAR167555:NATL1_17551-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26310; Mature: 26310

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 16-16 BINDING 37-37 BINDING 128-128 BINDING 190-190 BINDING 199-199 BINDING 219-219 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIDNPSEKIIIALDGMDKDDVVNLLKKIPEIVWVKVGLELFVSEGPDVLSMLREKGKK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE
IFLDLKFHDIPTTVARACFAASQTGAEFISLHTCAGMKALKMANEAANEGASKVNLIPPK
EEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEECCCHH
LLGITILTSWTRESFCNDLLINQSIDQRVKHLAEIASNSGLGGCVCSPKEVQFLRESYPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCH
TFELITPGIRSLGSNVNDQSRVSDASEAIKMGASKLVIGRAITQSNDSAYMFKSFCDKIS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHCC
I
C
>Mature Secondary Structure
MKNIDNPSEKIIIALDGMDKDDVVNLLKKIPEIVWVKVGLELFVSEGPDVLSMLREKGKK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE
IFLDLKFHDIPTTVARACFAASQTGAEFISLHTCAGMKALKMANEAANEGASKVNLIPPK
EEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEECCCHH
LLGITILTSWTRESFCNDLLINQSIDQRVKHLAEIASNSGLGGCVCSPKEVQFLRESYPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCH
TFELITPGIRSLGSNVNDQSRVSDASEAIKMGASKLVIGRAITQSNDSAYMFKSFCDKIS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA