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The map label for this gene is tyrS [H]

Identifier: 124026461

GI number: 124026461

Start: 1437029

End: 1438276

Strand: Reverse

Name: tyrS [H]

Synonym: NATL1_17561

Alternate gene names: 124026461

Gene position: 1438276-1437029 (Counterclockwise)

Preceding gene: 124026462

Following gene: 124026460

Centisome position: 77.13

GC content: 32.69

Gene sequence:

>1248_bases
ATGTTAGATAACATCAATGAATTACCTGATTGGATCTCTAGAGGGTTAGATGATCTCTTCCCTAATGATAATTCAGGAGA
TTCAGATCAAAGCTTTCTAAAAAGATTAGAACTTTCATCTGTAGATAAAAATCCTTTACGTGTAAAACTTGGAATTGATC
CAACAGGAACTGATATTCATATAGGTCATAGCATTCTTTTTAGAAAATTAAGAGCTTTCCAAGATGCTGGACATACGGCA
ATACTTATAATCGGAGATTTCACAGCAAGGATTGGAGACCCAACTGGAAAAAGTAAAACTAGAATTCAGCTTTCAAAAGA
TGAGGTTGAAGCAAATGCGTTGAATTACCTTGAGCAATTAGGACTTGGTAAAGAGCCCAAAGATTCATTACTTGATTTTT
CAACTCCCAGTCGCATAGAAATCAGAAGAAATAGTGAGTGGCTTGAAAATCTAAATTTGTCTAAAGTTATAGAACTTTTA
TCAAATTCAACAGTTGGTCAAATGCTGGCTAAGGAAGACTTTAGTAATCGATATAAATCTGGCACTCCTATATCTCTTCA
TGAATTTCTTTATCCGCTTCTTCAAGGATATGATTCTGTTGCGATCAATTCTGATGTGGAACTTGGTGGTACAGATCAAA
AATTTAATATTGCTATGGGGCGAGATCTTCAAAGAGCTTTTGGACAGAAACCCCAATTTGGAATGCTATTACCTATTTTA
GTTGGATTAGATGGGACACGAAAAATGAGTAAAAGCTTAGACAATATTGTAGGAATAAATGAAGATTCATTATCTATGTA
TTCAAAATTAGAAAAGGTTCCAGATGATTTGGTTCTTACATATTTAAATTTACTCACTAATGAAAACTTAAAAGAATTAA
GTTCAAATCCAAGAGAGGTTCAAAAATTTATGGCTTTAAAAATAACATCTAATTTTAAAGGGATTGAGGCAGCAAAAAAT
GCTCAATTTAATTCTGAAAAATTAGTTTTAGGTACTAAAGATTCTCTTGAGGAAATACCAGAAGCATCAGTTTCTAATGT
GAATTTTCCAGCTAAAGCATTTTATTTATTTAGTAAGATGGAAATGTGTTCAAGTAGCAGTGAAGCAAGAAGACAAATTC
TTGGAGGAGGAGTGAGAATTGATGGGGAAAAAATTATGGATCCTAATTTAGAGTTTCACACTCCAGATGATCTCATAGGA
AAAATATTACAAGTAGGAAAGAAGAAATTTCTTCGTGTTTCAACTTGA

Upstream 100 bases:

>100_bases
GGATTACTCCAGATCAAATGTTTGATCAGATGAATAAAACTTTGGTGAGAATGAAATCAGAATTGGATAAATGATTATTA
ACCTTTTTAATATTTAGAGT

Downstream 100 bases:

>100_bases
TAGATTGTTATGAAAAATATTGATAATCCAAGTGAAAAAATAATTATTGCTTTAGATGGAATGGATAAAGATGATGTTGT
CAATTTATTGAAAAAAATAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLDNINELPDWISRGLDDLFPNDNSGDSDQSFLKRLELSSVDKNPLRVKLGIDPTGTDIHIGHSILFRKLRAFQDAGHTA
ILIIGDFTARIGDPTGKSKTRIQLSKDEVEANALNYLEQLGLGKEPKDSLLDFSTPSRIEIRRNSEWLENLNLSKVIELL
SNSTVGQMLAKEDFSNRYKSGTPISLHEFLYPLLQGYDSVAINSDVELGGTDQKFNIAMGRDLQRAFGQKPQFGMLLPIL
VGLDGTRKMSKSLDNIVGINEDSLSMYSKLEKVPDDLVLTYLNLLTNENLKELSSNPREVQKFMALKITSNFKGIEAAKN
AQFNSEKLVLGTKDSLEEIPEASVSNVNFPAKAFYLFSKMEMCSSSSEARRQILGGGVRIDGEKIMDPNLEFHTPDDLIG
KILQVGKKKFLRVST

Sequences:

>Translated_415_residues
MLDNINELPDWISRGLDDLFPNDNSGDSDQSFLKRLELSSVDKNPLRVKLGIDPTGTDIHIGHSILFRKLRAFQDAGHTA
ILIIGDFTARIGDPTGKSKTRIQLSKDEVEANALNYLEQLGLGKEPKDSLLDFSTPSRIEIRRNSEWLENLNLSKVIELL
SNSTVGQMLAKEDFSNRYKSGTPISLHEFLYPLLQGYDSVAINSDVELGGTDQKFNIAMGRDLQRAFGQKPQFGMLLPIL
VGLDGTRKMSKSLDNIVGINEDSLSMYSKLEKVPDDLVLTYLNLLTNENLKELSSNPREVQKFMALKITSNFKGIEAAKN
AQFNSEKLVLGTKDSLEEIPEASVSNVNFPAKAFYLFSKMEMCSSSSEARRQILGGGVRIDGEKIMDPNLEFHTPDDLIG
KILQVGKKKFLRVST
>Mature_415_residues
MLDNINELPDWISRGLDDLFPNDNSGDSDQSFLKRLELSSVDKNPLRVKLGIDPTGTDIHIGHSILFRKLRAFQDAGHTA
ILIIGDFTARIGDPTGKSKTRIQLSKDEVEANALNYLEQLGLGKEPKDSLLDFSTPSRIEIRRNSEWLENLNLSKVIELL
SNSTVGQMLAKEDFSNRYKSGTPISLHEFLYPLLQGYDSVAINSDVELGGTDQKFNIAMGRDLQRAFGQKPQFGMLLPIL
VGLDGTRKMSKSLDNIVGINEDSLSMYSKLEKVPDDLVLTYLNLLTNENLKELSSNPREVQKFMALKITSNFKGIEAAKN
AQFNSEKLVLGTKDSLEEIPEASVSNVNFPAKAFYLFSKMEMCSSSSEARRQILGGGVRIDGEKIMDPNLEFHTPDDLIG
KILQVGKKKFLRVST

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=435, Percent_Identity=27.3563218390805, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1787925, Length=401, Percent_Identity=28.1795511221945, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=312, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=422, Percent_Identity=27.0142180094787, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=25.3694581280788, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46247; Mature: 46247

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNINELPDWISRGLDDLFPNDNSGDSDQSFLKRLELSSVDKNPLRVKLGIDPTGTDIH
CCCCHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
IGHSILFRKLRAFQDAGHTAILIIGDFTARIGDPTGKSKTRIQLSKDEVEANALNYLEQL
CCHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
GLGKEPKDSLLDFSTPSRIEIRRNSEWLENLNLSKVIELLSNSTVGQMLAKEDFSNRYKS
CCCCCCHHHHCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
GTPISLHEFLYPLLQGYDSVAINSDVELGGTDQKFNIAMGRDLQRAFGQKPQFGMLLPIL
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEHHCCHHHHHCCCCCCCCHHHHHH
VGLDGTRKMSKSLDNIVGINEDSLSMYSKLEKVPDDLVLTYLNLLTNENLKELSSNPREV
HCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCHHHH
QKFMALKITSNFKGIEAAKNAQFNSEKLVLGTKDSLEEIPEASVSNVNFPAKAFYLFSKM
HHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EMCSSSSEARRQILGGGVRIDGEKIMDPNLEFHTPDDLIGKILQVGKKKFLRVST
HHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MLDNINELPDWISRGLDDLFPNDNSGDSDQSFLKRLELSSVDKNPLRVKLGIDPTGTDIH
CCCCHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
IGHSILFRKLRAFQDAGHTAILIIGDFTARIGDPTGKSKTRIQLSKDEVEANALNYLEQL
CCHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
GLGKEPKDSLLDFSTPSRIEIRRNSEWLENLNLSKVIELLSNSTVGQMLAKEDFSNRYKS
CCCCCCHHHHCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
GTPISLHEFLYPLLQGYDSVAINSDVELGGTDQKFNIAMGRDLQRAFGQKPQFGMLLPIL
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEHHCCHHHHHCCCCCCCCHHHHHH
VGLDGTRKMSKSLDNIVGINEDSLSMYSKLEKVPDDLVLTYLNLLTNENLKELSSNPREV
HCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCHHHH
QKFMALKITSNFKGIEAAKNAQFNSEKLVLGTKDSLEEIPEASVSNVNFPAKAFYLFSKM
HHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EMCSSSSEARRQILGGGVRIDGEKIMDPNLEFHTPDDLIGKILQVGKKKFLRVST
HHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA