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The map label for this gene is miaB

Identifier: 124026430

GI number: 124026430

Start: 1411645

End: 1413036

Strand: Direct

Name: miaB

Synonym: NATL1_17251

Alternate gene names: 124026430

Gene position: 1411645-1413036 (Clockwise)

Preceding gene: 124026427

Following gene: 124026431

Centisome position: 75.7

GC content: 35.06

Gene sequence:

>1392_bases
ATGACAACAGTTCAGCTCCAAAAAACAAATCAACCTCCAGTTAAGGCCCCAAGAGGTAGCTATTGGATTACAACTTTTGG
ATGTCAAATGAACAAAGCTGACTCAGAAAGGATGTCAGGAATACTTGAATATATGGGTTATTACCCTGCAGAAGAAGAAC
TTAAGGCAGATTTGGTTCTTTATAACACATGTACTATTCGCGATAGTGCAGAGCAAAAAGTTTATAGCTACCTCGGAAGA
CAGGCAATAAGAAAAAGATCATTACCAAATCTAAAAATTGTCGTTGCAGGTTGCTTGGCACAGCAAGAAGGAGAATCTCT
TTTAAGGCGAGTCCCAGAAATAGATCTTCTAATGGGACCTCAACATTGCAATCGTCTAGAAAGTTTGCTCAATCAAGTTG
ATAGTGGCCAACAAGTTCTTGCTACTGAAGAACAATTTATACTTGAGGACATAACAACTCCAAGAAGAGATAGTTCCTTT
TGCGGATGGGTAAATATTATTTACGGATGTAATGAGCGTTGCACATATTGTGTGGTTCCTTCTGTGAGAGGAAAAGAGCA
ATCAAGAACACCTGAAGCTATTAAATCAGAAGTTGAGGATTTAGCAAAAAGTGGTTATAAAGAGATAACTCTTCTGGGAC
AGAATATAGATGCTTATGGCAGAGACTTTCAAAGTCAAAACAAAGAAGCTTCTGCACAAATAACACTATCTTATTTATTA
AAATATATTCATGATATTGAAGGGATTGAACGTATTAGATTTGCAACAAGTCATCCTCGCTACTTTACTAAAGAATTAAT
AGATACTTGCTCAGAACTGCCAAAAGTCTGCGAACACTTTCACATCCCATTTCAAAGTGGAAGTAATAAGATTCTCAAAA
ACATGGGGCGGGGCTATACAATTGAAAGCTATAAAAATATTATTAATTACATCAAGTCTAAAATACCTAAAGCAGCAATA
AGTGGGGATGCAATCGTAGCTTTTCCAGGAGAAAGTGAAACAGATTATGAACAAACTTTATCATTAATCGATGAAATAAA
ATTTGATCATGTCAATACAGCCGCATACTCTCCCAGACCAAATACTCCAGCAGCAACGTGGCCTAGACAATTAAACGAAG
ATATAAAAGTAAAGAGACTTAGAGAAATAAATAGTTTGGTTGAAAATATAGCTAAAGAAAGAAATCAAAGATATAAAAAT
ACATCTCAAGAAATACTTATTGAGAATATTAATCCAAAAGATAGTTTTCAATTAATGGGACGAACACGTACAAACCGTCT
CACTTTTTTCCCAAGATCATTAGAAAATGGAGTAGAAAACAAACTTGGTGAACTTATCAAGGTAAAGATTACTGATGTTC
GTCCTTTTTCTTTAACAGCCAAATTACTTTGA

Upstream 100 bases:

>100_bases
GAATCCCCTCATCCGCCCTCAAATCCTTTTTTTATTTAAATCCCTTTAAAAAAATTTCCTAGAATAATTTTATTTCAAAG
AAAAAATATATAGCTTCACG

Downstream 100 bases:

>100_bases
AAAGATCTTACATATCAAAATTGATACATAATTAAAAAGATAAATTCATTAAACATGTTTTCAAATAAACCAGTTATAGG
AGTTGTTTTTGGAGGCAAAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTTVQLQKTNQPPVKAPRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVLYNTCTIRDSAEQKVYSYLGR
QAIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGPQHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSF
CGWVNIIYGCNERCTYCVVPSVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEASAQITLSYLL
KYIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYTIESYKNIINYIKSKIPKAAI
SGDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRPNTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKN
TSQEILIENINPKDSFQLMGRTRTNRLTFFPRSLENGVENKLGELIKVKITDVRPFSLTAKLL

Sequences:

>Translated_463_residues
MTTVQLQKTNQPPVKAPRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVLYNTCTIRDSAEQKVYSYLGR
QAIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGPQHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSF
CGWVNIIYGCNERCTYCVVPSVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEASAQITLSYLL
KYIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYTIESYKNIINYIKSKIPKAAI
SGDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRPNTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKN
TSQEILIENINPKDSFQLMGRTRTNRLTFFPRSLENGVENKLGELIKVKITDVRPFSLTAKLL
>Mature_462_residues
TTVQLQKTNQPPVKAPRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVLYNTCTIRDSAEQKVYSYLGRQ
AIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGPQHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSFC
GWVNIIYGCNERCTYCVVPSVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEASAQITLSYLLK
YIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYTIESYKNIINYIKSKIPKAAIS
GDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRPNTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKNT
SQEILIENINPKDSFQLMGRTRTNRLTFFPRSLENGVENKLGELIKVKITDVRPFSLTAKLL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=31.9238900634249, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=31.9238900634249, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI93277076, Length=378, Percent_Identity=28.8359788359788, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=37.8923766816144, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=28.3185840707965, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=32.1276595744681, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=373, Percent_Identity=25.4691689008043, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356207, Length=436, Percent_Identity=31.1926605504587, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=26.5795206971678, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROM1 (A2C471)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015545.1
- ProteinModelPortal:   A2C471
- SMR:   A2C471
- STRING:   A2C471
- GeneID:   4779986
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_17251
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14333
- BioCyc:   PMAR167555:NATL1_17251-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52806; Mature: 52675

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVQLQKTNQPPVKAPRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVL
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEE
YNTCTIRDSAEQKVYSYLGRQAIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGP
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEECH
QHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSFCGWVNIIYGCNERCTYCVVP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCEEEECC
SVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEASAQITLSYLL
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHCCCCCHHHHHHHHHH
KYIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
IESYKNIINYIKSKIPKAAISGDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRP
HHHHHHHHHHHHHHCCCHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKNTSQEILIENINPKDSFQLMG
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHC
RTRTNRLTFFPRSLENGVENKLGELIKVKITDVRPFSLTAKLL
CCCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCHHEECC
>Mature Secondary Structure 
TTVQLQKTNQPPVKAPRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVL
CEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEE
YNTCTIRDSAEQKVYSYLGRQAIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGP
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEECH
QHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSFCGWVNIIYGCNERCTYCVVP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCCCEEEECC
SVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEASAQITLSYLL
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHCCCCCHHHHHHHHHH
KYIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
IESYKNIINYIKSKIPKAAISGDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRP
HHHHHHHHHHHHHHCCCHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKNTSQEILIENINPKDSFQLMG
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHC
RTRTNRLTFFPRSLENGVENKLGELIKVKITDVRPFSLTAKLL
CCCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA