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The map label for this gene is ddl

Identifier: 124026431

GI number: 124026431

Start: 1413091

End: 1414137

Strand: Direct

Name: ddl

Synonym: NATL1_17261

Alternate gene names: 124026431

Gene position: 1413091-1414137 (Clockwise)

Preceding gene: 124026430

Following gene: 124026432

Centisome position: 75.78

GC content: 31.52

Gene sequence:

>1047_bases
ATGTTTTCAAATAAACCAGTTATAGGAGTTGTTTTTGGAGGCAAATCTAGTGAGCATGAAGTATCTATAAAATCCGCAAA
AACTATTTACAATGCTTTAAGCCATTTAAGTAATAAGGAACGTTATGTAGCAAGACCAGTTTATATTGATAAGTACGGTT
ATTGGCATGACTATATATTTTCTGAATCCATTTTATATGATAAAAAAGATCATTTAATCTTTGAAGATAGAAGAGTTAAT
CTCACTAATTTATCAAACATGGAAGATATTGATGTCTGGTTCCCTTGTCTTCATGGACCAAATGGAGAGGATGGTGTAAT
CCAAGGACTTTTTAAATCAACTGGAAAACCATTTGTAGGCTCGGGTGTTCTCGGGTCCGCATTAGGTATGGATAAAATAG
CAATGAAATCAGTATTTAAATCATATAATTTACCTCAAGTTCCTTATGTAAGCTTAAACAAAGCTGATATCCAAAATAAT
TTATATATGAAATCTATTTATGAACAAATAAATAAAATAATTAATTATCCTTGTTTTATTAAACCTGCAAATCTAGGTTC
TTCTGTCGGCATAACAAAAGCATACTCAAAAGAAGAATTTATTACTGGAATAGAATTTGCTGCAAAATATGATGAGAGAA
TTATAGTTGAAAAAAGTATAGAAGGTAGAGAACTTGAATGCGGTGTTTTAGGCAAATCAATCATGAAATCATCTGTTGTT
GGGGAAGTTAAATTTCAAACTGATTGGTATACATATGAGTCTAAATATAATGCGAATCTAAGCAGTACAATTATCCCAGC
TGATTTGAATATAGAGATATCCAATAAGATACAAAAGCTAGCTATCGAGGCCTGTAAAGCTATTAATGCTTATGGTTTAG
CAAGAGTCGATTTCTTTTATCAAGAAAGCACGCAACAGATTTATATAAATGAAGTTAACACCTTGCCAGGATTCACTAAA
ACAAGCATGTACCCGACATTGTGGGAAGCTTCTGGATTAAAACTAGAAAAACTTGTTGCTAGTCTCATAGAAATAGCTAA
AGAATGA

Upstream 100 bases:

>100_bases
GATTACTGATGTTCGTCCTTTTTCTTTAACAGCCAAATTACTTTGAAAAGATCTTACATATCAAAATTGATACATAATTA
AAAAGATAAATTCATTAAAC

Downstream 100 bases:

>100_bases
ATTAACCATGACATTTTTAGATATCTTTTTCAAAAAATGATTCACGGTCTCCTTTGGTTGCCATTACTAATGGCTTTTAT
CCTCATAGCATCCCTTGGAT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MFSNKPVIGVVFGGKSSEHEVSIKSAKTIYNALSHLSNKERYVARPVYIDKYGYWHDYIFSESILYDKKDHLIFEDRRVN
LTNLSNMEDIDVWFPCLHGPNGEDGVIQGLFKSTGKPFVGSGVLGSALGMDKIAMKSVFKSYNLPQVPYVSLNKADIQNN
LYMKSIYEQINKIINYPCFIKPANLGSSVGITKAYSKEEFITGIEFAAKYDERIIVEKSIEGRELECGVLGKSIMKSSVV
GEVKFQTDWYTYESKYNANLSSTIIPADLNIEISNKIQKLAIEACKAINAYGLARVDFFYQESTQQIYINEVNTLPGFTK
TSMYPTLWEASGLKLEKLVASLIEIAKE

Sequences:

>Translated_348_residues
MFSNKPVIGVVFGGKSSEHEVSIKSAKTIYNALSHLSNKERYVARPVYIDKYGYWHDYIFSESILYDKKDHLIFEDRRVN
LTNLSNMEDIDVWFPCLHGPNGEDGVIQGLFKSTGKPFVGSGVLGSALGMDKIAMKSVFKSYNLPQVPYVSLNKADIQNN
LYMKSIYEQINKIINYPCFIKPANLGSSVGITKAYSKEEFITGIEFAAKYDERIIVEKSIEGRELECGVLGKSIMKSSVV
GEVKFQTDWYTYESKYNANLSSTIIPADLNIEISNKIQKLAIEACKAINAYGLARVDFFYQESTQQIYINEVNTLPGFTK
TSMYPTLWEASGLKLEKLVASLIEIAKE
>Mature_348_residues
MFSNKPVIGVVFGGKSSEHEVSIKSAKTIYNALSHLSNKERYVARPVYIDKYGYWHDYIFSESILYDKKDHLIFEDRRVN
LTNLSNMEDIDVWFPCLHGPNGEDGVIQGLFKSTGKPFVGSGVLGSALGMDKIAMKSVFKSYNLPQVPYVSLNKADIQNN
LYMKSIYEQINKIINYPCFIKPANLGSSVGITKAYSKEEFITGIEFAAKYDERIIVEKSIEGRELECGVLGKSIMKSSVV
GEVKFQTDWYTYESKYNANLSSTIIPADLNIEISNKIQKLAIEACKAINAYGLARVDFFYQESTQQIYINEVNTLPGFTK
TSMYPTLWEASGLKLEKLVASLIEIAKE

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=38.375350140056, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1786280, Length=339, Percent_Identity=30.0884955752212, Blast_Score=164, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PROM1 (A2C472)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015546.1
- ProteinModelPortal:   A2C472
- SMR:   A2C472
- STRING:   A2C472
- GeneID:   4779645
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_17261
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   CKAINAY
- ProtClustDB:   PRK01966
- BioCyc:   PMAR167555:NATL1_17261-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39196; Mature: 39196

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSNKPVIGVVFGGKSSEHEVSIKSAKTIYNALSHLSNKERYVARPVYIDKYGYWHDYIF
CCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCHHHHHH
SESILYDKKDHLIFEDRRVNLTNLSNMEDIDVWFPCLHGPNGEDGVIQGLFKSTGKPFVG
CCCCEECCCCCEEEECCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCC
SGVLGSALGMDKIAMKSVFKSYNLPQVPYVSLNKADIQNNLYMKSIYEQINKIINYPCFI
CCCHHHHCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCHHHHHHHHHHHHHHCCCCEE
KPANLGSSVGITKAYSKEEFITGIEFAAKYDERIIVEKSIEGRELECGVLGKSIMKSSVV
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHHC
GEVKFQTDWYTYESKYNANLSSTIIPADLNIEISNKIQKLAIEACKAINAYGLARVDFFY
EEEEEEEEEEEECCEECCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEE
QESTQQIYINEVNTLPGFTKTSMYPTLWEASGLKLEKLVASLIEIAKE
ECCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSNKPVIGVVFGGKSSEHEVSIKSAKTIYNALSHLSNKERYVARPVYIDKYGYWHDYIF
CCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCHHHHHH
SESILYDKKDHLIFEDRRVNLTNLSNMEDIDVWFPCLHGPNGEDGVIQGLFKSTGKPFVG
CCCCEECCCCCEEEECCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCC
SGVLGSALGMDKIAMKSVFKSYNLPQVPYVSLNKADIQNNLYMKSIYEQINKIINYPCFI
CCCHHHHCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCHHHHHHHHHHHHHHCCCCEE
KPANLGSSVGITKAYSKEEFITGIEFAAKYDERIIVEKSIEGRELECGVLGKSIMKSSVV
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHHC
GEVKFQTDWYTYESKYNANLSSTIIPADLNIEISNKIQKLAIEACKAINAYGLARVDFFY
EEEEEEEEEEEECCEECCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEE
QESTQQIYINEVNTLPGFTKTSMYPTLWEASGLKLEKLVASLIEIAKE
ECCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA