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The map label for this gene is rsmD [H]

Identifier: 124026233

GI number: 124026233

Start: 1243789

End: 1244367

Strand: Direct

Name: rsmD [H]

Synonym: NATL1_15271

Alternate gene names: 124026233

Gene position: 1243789-1244367 (Clockwise)

Preceding gene: 124026232

Following gene: 124026235

Centisome position: 66.7

GC content: 33.51

Gene sequence:

>579_bases
ATGTCTGGGAAAAGGATAGAAAGTCCTTTAAGCCAAAAGACAAGACCAACCTCCTCAAAAGTAAGAGAGGCACTAATAAA
TATTCTTGGGAACAAGCTTAGAGGAGCAAGTTGGCTAGATCTTTGCAGTGGGAGTGGAGCAATGGCATGTGAGGCTCTGC
AAAAAGGAGTAAAGAGGGTTCTTGCAATTGAAAACCAGAGGGAAACTGCAAAATTATGCAAAAAAAACCTTATGGATATA
TCAAATACTATGAATCAATCAATACATATTGAAGTCATTTGCAATGAATTGATCTCATTTCTCAAAAAAGGACCAAAAAA
TCAAAAAATTCAGTTTGTTAAAGATTGCCAAAATCCTGAAAAATTTGATTTTGTTTTTCTAGATCCACCATACGAATCTG
AAGTATATCTAATTACTCAAGAACTTTTATTATCTAAAGAATGGATTAAAGAATCAACTACTTTGATATGCGAATGCTCG
TCAAAATCAATGCCAAGAATACATAATGGGTGGAAATTAAATAAAGAAAAATTTTATGGAAGCACCTGTCTCATTTTTCT
TATTCCCAATCAGGCATAG

Upstream 100 bases:

>100_bases
CATCCCGAAAAGTCAGGAGTTGCAGGACAAAAAATTATTTTTAATTGGATTAATTGGTTAAAAAAAAACAAATTTTAGTT
TGAAAAGGAAATTGAAATTA

Downstream 100 bases:

>100_bases
AACTGCTCCGACGGTACTGATTCCATGCACTAACAAAAAGGCCTAATAATGTTACGGGAACTAGTCCAAGAACAATCCCA
CATAGCAGAGGCTCAATCAT

Product: N6-adenine-specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSGKRIESPLSQKTRPTSSKVREALINILGNKLRGASWLDLCSGSGAMACEALQKGVKRVLAIENQRETAKLCKKNLMDI
SNTMNQSIHIEVICNELISFLKKGPKNQKIQFVKDCQNPEKFDFVFLDPPYESEVYLITQELLLSKEWIKESTTLICECS
SKSMPRIHNGWKLNKEKFYGSTCLIFLIPNQA

Sequences:

>Translated_192_residues
MSGKRIESPLSQKTRPTSSKVREALINILGNKLRGASWLDLCSGSGAMACEALQKGVKRVLAIENQRETAKLCKKNLMDI
SNTMNQSIHIEVICNELISFLKKGPKNQKIQFVKDCQNPEKFDFVFLDPPYESEVYLITQELLLSKEWIKESTTLICECS
SKSMPRIHNGWKLNKEKFYGSTCLIFLIPNQA
>Mature_191_residues
SGKRIESPLSQKTRPTSSKVREALINILGNKLRGASWLDLCSGSGAMACEALQKGVKRVLAIENQRETAKLCKKNLMDIS
NTMNQSIHIEVICNELISFLKKGPKNQKIQFVKDCQNPEKFDFVFLDPPYESEVYLITQELLLSKEWIKESTTLICECSS
KSMPRIHNGWKLNKEKFYGSTCLIFLIPNQA

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21788; Mature: 21656

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKRIESPLSQKTRPTSSKVREALINILGNKLRGASWLDLCSGSGAMACEALQKGVKRV
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHH
LAIENQRETAKLCKKNLMDISNTMNQSIHIEVICNELISFLKKGPKNQKIQFVKDCQNPE
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
KFDFVFLDPPYESEVYLITQELLLSKEWIKESTTLICECSSKSMPRIHNGWKLNKEKFYG
CEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECHHHCCC
STCLIFLIPNQA
CEEEEEEECCCC
>Mature Secondary Structure 
SGKRIESPLSQKTRPTSSKVREALINILGNKLRGASWLDLCSGSGAMACEALQKGVKRV
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHH
LAIENQRETAKLCKKNLMDISNTMNQSIHIEVICNELISFLKKGPKNQKIQFVKDCQNPE
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
KFDFVFLDPPYESEVYLITQELLLSKEWIKESTTLICECSSKSMPRIHNGWKLNKEKFYG
CEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECHHHCCC
STCLIFLIPNQA
CEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]