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The map label for this gene is hisH [H]

Identifier: 124026232

GI number: 124026232

Start: 1243137

End: 1243766

Strand: Direct

Name: hisH [H]

Synonym: NATL1_15261

Alternate gene names: 124026232

Gene position: 1243137-1243766 (Clockwise)

Preceding gene: 124026231

Following gene: 124026233

Centisome position: 66.67

GC content: 33.65

Gene sequence:

>630_bases
TTGACAAAAATTGGATTAATAGATTATGGAATGGGTAATCTTTTTTCCGTTCAGCAAGCATTTAAAAGGTTTAATCAGCC
TTTAGATATTATTTCTGATATCAAAACACTTCAGTCTTCTGATGCTTTAATTCTTCCCGGAGTTGGTGCTTTTGATCCCG
CAATGATGAATTTAAGAAAAACTGAACTAGTACCTTCAATAATTGACTGGATAAATAATGGCAAACCACTTTTTGGAATT
TGTTTAGGATTACAGCTTTTATTTGAGACAAGTGATGAGGGGACTTCAGAAGGATTAGGAGTTATCAAAGGTCATATTCG
CAGATTACCTCAAGAAAAAGACGAAAGGATTCCTCATATTGGTTGGTCTCCAATATATAAAACAAATGAATGTCCTATTC
TAGAAAATTATCCTGGTTCTAATTGGATGTATTTCGTCCATTCCTACTCAGCATGTCCTTTAGAACCAAAACATACCGTA
GCCGTAACAAAATTTGGTAAAACTGATGTCTCATCTATGGTCTGGCATAAAAATACTGGTGCCTGTCAGTTTCATCCCGA
AAAGTCAGGAGTTGCAGGACAAAAAATTATTTTTAATTGGATTAATTGGTTAAAAAAAAACAAATTTTAG

Upstream 100 bases:

>100_bases
AGTCCCTAAAGCCACCCTCTCAGGAACAATTTCCAAACATCTCTAATCCCAAAATTTAGAAGTTCTAGAGCCCAATTCAA
ATGATTCATAGGTAAATCTT

Downstream 100 bases:

>100_bases
TTTGAAAAGGAAATTGAAATTAATGTCTGGGAAAAGGATAGAAAGTCCTTTAAGCCAAAAGACAAGACCAACCTCCTCAA
AAGTAAGAGAGGCACTAATA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTKIGLIDYGMGNLFSVQQAFKRFNQPLDIISDIKTLQSSDALILPGVGAFDPAMMNLRKTELVPSIIDWINNGKPLFGI
CLGLQLLFETSDEGTSEGLGVIKGHIRRLPQEKDERIPHIGWSPIYKTNECPILENYPGSNWMYFVHSYSACPLEPKHTV
AVTKFGKTDVSSMVWHKNTGACQFHPEKSGVAGQKIIFNWINWLKKNKF

Sequences:

>Translated_209_residues
MTKIGLIDYGMGNLFSVQQAFKRFNQPLDIISDIKTLQSSDALILPGVGAFDPAMMNLRKTELVPSIIDWINNGKPLFGI
CLGLQLLFETSDEGTSEGLGVIKGHIRRLPQEKDERIPHIGWSPIYKTNECPILENYPGSNWMYFVHSYSACPLEPKHTV
AVTKFGKTDVSSMVWHKNTGACQFHPEKSGVAGQKIIFNWINWLKKNKF
>Mature_208_residues
TKIGLIDYGMGNLFSVQQAFKRFNQPLDIISDIKTLQSSDALILPGVGAFDPAMMNLRKTELVPSIIDWINNGKPLFGIC
LGLQLLFETSDEGTSEGLGVIKGHIRRLPQEKDERIPHIGWSPIYKTNECPILENYPGSNWMYFVHSYSACPLEPKHTVA
VTKFGKTDVSSMVWHKNTGACQFHPEKSGVAGQKIIFNWINWLKKNKF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=33.502538071066, Blast_Score=100, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=33.1797235023041, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23541; Mature: 23409

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIGLIDYGMGNLFSVQQAFKRFNQPLDIISDIKTLQSSDALILPGVGAFDPAMMNLRK
CCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
TELVPSIIDWINNGKPLFGICLGLQLLFETSDEGTSEGLGVIKGHIRRLPQEKDERIPHI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCC
GWSPIYKTNECPILENYPGSNWMYFVHSYSACPLEPKHTVAVTKFGKTDVSSMVWHKNTG
CCCCCEECCCCCEECCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHEECCCC
ACQFHPEKSGVAGQKIIFNWINWLKKNKF
CEEECCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIGLIDYGMGNLFSVQQAFKRFNQPLDIISDIKTLQSSDALILPGVGAFDPAMMNLRK
CEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
TELVPSIIDWINNGKPLFGICLGLQLLFETSDEGTSEGLGVIKGHIRRLPQEKDERIPHI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCC
GWSPIYKTNECPILENYPGSNWMYFVHSYSACPLEPKHTVAVTKFGKTDVSSMVWHKNTG
CCCCCEECCCCCEECCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHEECCCC
ACQFHPEKSGVAGQKIIFNWINWLKKNKF
CEEECCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA