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The map label for this gene is leuC

Identifier: 124025047

GI number: 124025047

Start: 307783

End: 309192

Strand: Reverse

Name: leuC

Synonym: NATL1_03341

Alternate gene names: 124025047

Gene position: 309192-307783 (Counterclockwise)

Preceding gene: 124025048

Following gene: 124025046

Centisome position: 16.58

GC content: 40.07

Gene sequence:

>1410_bases
TTGAGTTCTAGAACCCTCTACGACAAAGTTTGGAACTTCCATCAGGTAAAAGAATTACCTGGAGGATCAACTCAACTTTT
TATTGGTCTTCATTTAATTCACGAAGTTACAAGTCCTCAGGCTTTCTCTGCACTTAATGAAAAAAAACTTGGAGTAAAGT
TTCCCAATCTTACTGTCGCAACAGTTGACCATATAGTTCCGACTTCAAACCAGCAACGTCCTTTTAGTGATCCTCTTGCC
GAGGAAATGTTGTCTACTTTAGAAAAAAACTGCAAAACTCATGGGATAAAATTTCACGGAATTGGAAGTAATTCGCAAGG
AGTAGTACATGTTATGGCTCCAGAATTAGGATTAACCCAGCCTGGAATGACAGTAGCCTGCGGAGATTCGCATACCTCAA
CCCATGGAGCATTTGGAGCAATTGCCTTTGGAATTGGAACTAGTCAAGTGCGAGATGTTTTAGCCAGTCAAAGCTTGGCA
ATGAATAAATTAAAAGTAAGAAGAATATGGGTAGAGGGTGAATTGCAAAAGGGAGTCTATGCAAAAGACCTAATTCTTCA
TATCATTCGTCATCTTGGAGTTAAAGGAGGTGTTGGATTTGCATATGAATTTGCTGGGCCTGCAATAGAAAAACTCTCAA
TGGAGGGACGAATGACCATATGCAATATGGCTATTGAAGGTGGTGCAAGATGCGGTTATATCAATCCAGATGAAACCACT
TTCAAATATTTAAAAGGGAAAGAACATGCGCCCAAAGGTCAAGAATGGGATAAGGCGATTTCTTGGTGGAAAAGTTTAGC
TAGTGATTCAAAAGCAACATTCGATGATGAGATTCAATTAGATGGATCATCAATCGAACCCACTGTTACTTGGGGTATAA
CTCCTGGGCAAGGAATTTCAATCAAAGAAACAATTCCAAACCCTGAGTTTCTTCCCAAAAATGAGCAACAAATCGCTAAA
GACGCATGCAAATACATGAATCTAAAACCAGACGAACCCATAGAGGGACAATCAATTGATGTTTGCTTTATAGGGAGCTG
TACTAATGGAAGATTAAGTGATCTCCAAGAGGCATCTAAAATTGTTAAGGGTAATACAGTAGCTGATGGGATTAGAGCAT
TTGTCGTTCCTGGTTCTCAAAAGGTCGCTAAGGAGGCAAAAGAAAAAGGATTAGATAAAATATTTCTTAAAGCAGGTTTT
GAGTGGCGAGAACCAGGTTGTTCAATGTGTCTAGCCATGAACCCAGACAAACTAGAAGGCAGACAAATAAGCGCTAGTTC
GAGTAATAGAAATTTCAAAGGAAGACAAGGCTCCGCAAAAGGGAGAACCTTGCTAATGAGCCCCGCTATGGTTGCTGCTG
CTGCAATAAATGGGAGGGTTACAGATGTAAGAAAGTTCCTGCAAGAGTAA

Upstream 100 bases:

>100_bases
CAAGAGAAGATATTCAAAAATTAAGCGTTTTAAATGCTCTTGATAGATTACGTTTATCTATAATCATGGCAAATTAAGTC
CTCATTATTGGATTTGAAAG

Downstream 100 bases:

>100_bases
AACTAAAAATAAACTTATTGAAAGATGAATCAAAAGTTCCCAAAAGGTGAAATCAAATCAATACAAGGCCGCAGCTTTAC
AATGAAAGGCGATGATATTG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSSRTLYDKVWNFHQVKELPGGSTQLFIGLHLIHEVTSPQAFSALNEKKLGVKFPNLTVATVDHIVPTSNQQRPFSDPLA
EEMLSTLEKNCKTHGIKFHGIGSNSQGVVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVEGELQKGVYAKDLILHIIRHLGVKGGVGFAYEFAGPAIEKLSMEGRMTICNMAIEGGARCGYINPDETT
FKYLKGKEHAPKGQEWDKAISWWKSLASDSKATFDDEIQLDGSSIEPTVTWGITPGQGISIKETIPNPEFLPKNEQQIAK
DACKYMNLKPDEPIEGQSIDVCFIGSCTNGRLSDLQEASKIVKGNTVADGIRAFVVPGSQKVAKEAKEKGLDKIFLKAGF
EWREPGCSMCLAMNPDKLEGRQISASSSNRNFKGRQGSAKGRTLLMSPAMVAAAAINGRVTDVRKFLQE

Sequences:

>Translated_469_residues
MSSRTLYDKVWNFHQVKELPGGSTQLFIGLHLIHEVTSPQAFSALNEKKLGVKFPNLTVATVDHIVPTSNQQRPFSDPLA
EEMLSTLEKNCKTHGIKFHGIGSNSQGVVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVEGELQKGVYAKDLILHIIRHLGVKGGVGFAYEFAGPAIEKLSMEGRMTICNMAIEGGARCGYINPDETT
FKYLKGKEHAPKGQEWDKAISWWKSLASDSKATFDDEIQLDGSSIEPTVTWGITPGQGISIKETIPNPEFLPKNEQQIAK
DACKYMNLKPDEPIEGQSIDVCFIGSCTNGRLSDLQEASKIVKGNTVADGIRAFVVPGSQKVAKEAKEKGLDKIFLKAGF
EWREPGCSMCLAMNPDKLEGRQISASSSNRNFKGRQGSAKGRTLLMSPAMVAAAAINGRVTDVRKFLQE
>Mature_468_residues
SSRTLYDKVWNFHQVKELPGGSTQLFIGLHLIHEVTSPQAFSALNEKKLGVKFPNLTVATVDHIVPTSNQQRPFSDPLAE
EMLSTLEKNCKTHGIKFHGIGSNSQGVVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAM
NKLKVRRIWVEGELQKGVYAKDLILHIIRHLGVKGGVGFAYEFAGPAIEKLSMEGRMTICNMAIEGGARCGYINPDETTF
KYLKGKEHAPKGQEWDKAISWWKSLASDSKATFDDEIQLDGSSIEPTVTWGITPGQGISIKETIPNPEFLPKNEQQIAKD
ACKYMNLKPDEPIEGQSIDVCFIGSCTNGRLSDLQEASKIVKGNTVADGIRAFVVPGSQKVAKEAKEKGLDKIFLKAGFE
WREPGCSMCLAMNPDKLEGRQISASSSNRNFKGRQGSAKGRTLLMSPAMVAAAAINGRVTDVRKFLQE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=394, Percent_Identity=26.9035532994924, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI8659555, Length=387, Percent_Identity=24.2894056847545, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI41352693, Length=369, Percent_Identity=25.4742547425474, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=51.6129032258064, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=24.7933884297521, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081781, Length=361, Percent_Identity=25.207756232687, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=409, Percent_Identity=28.361858190709, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI25149337, Length=390, Percent_Identity=28.974358974359, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.3375394321767, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=372, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=51.271186440678, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322261, Length=409, Percent_Identity=29.3398533007335, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=452, Percent_Identity=26.5486725663717, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323335, Length=389, Percent_Identity=26.7352185089974, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=390, Percent_Identity=27.6923076923077, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=390, Percent_Identity=27.6923076923077, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161076999, Length=390, Percent_Identity=27.6923076923077, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571643, Length=389, Percent_Identity=27.2493573264781, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=29.4594594594595, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17137564, Length=377, Percent_Identity=27.0557029177719, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PROM1 (A2C088)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014163.1
- ProteinModelPortal:   A2C088
- SMR:   A2C088
- STRING:   A2C088
- GeneID:   4779767
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03341
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   PMAR167555:NATL1_03341-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51033; Mature: 50902

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRTLYDKVWNFHQVKELPGGSTQLFIGLHLIHEVTSPQAFSALNEKKLGVKFPNLTVA
CCCCCHHHHHCCHHHHHHCCCCCEEEEHHHHHHHHHCCCHHHHHHCCCCCCEECCCEEEE
TVDHIVPTSNQQRPFSDPLAEEMLSTLEKNCKTHGIKFHGIGSNSQGVVHVMAPELGLTQ
EHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVEGELQKGVY
CCCEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCH
AKDLILHIIRHLGVKGGVGFAYEFAGPAIEKLSMEGRMTICNMAIEGGARCGYINPDETT
HHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHH
FKYLKGKEHAPKGQEWDKAISWWKSLASDSKATFDDEIQLDGSSIEPTVTWGITPGQGIS
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCE
IKETIPNPEFLPKNEQQIAKDACKYMNLKPDEPIEGQSIDVCFIGSCTNGRLSDLQEASK
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IVKGNTVADGIRAFVVPGSQKVAKEAKEKGLDKIFLKAGFEWREPGCSMCLAMNPDKLEG
HHCCCCHHCCCEEEEECCCHHHHHHHHHCCHHEEHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSAKGRTLLMSPAMVAAAAINGRVTDVRKFLQE
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure 
SSRTLYDKVWNFHQVKELPGGSTQLFIGLHLIHEVTSPQAFSALNEKKLGVKFPNLTVA
CCCCHHHHHCCHHHHHHCCCCCEEEEHHHHHHHHHCCCHHHHHHCCCCCCEECCCEEEE
TVDHIVPTSNQQRPFSDPLAEEMLSTLEKNCKTHGIKFHGIGSNSQGVVHVMAPELGLTQ
EHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVEGELQKGVY
CCCEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCH
AKDLILHIIRHLGVKGGVGFAYEFAGPAIEKLSMEGRMTICNMAIEGGARCGYINPDETT
HHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHH
FKYLKGKEHAPKGQEWDKAISWWKSLASDSKATFDDEIQLDGSSIEPTVTWGITPGQGIS
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCE
IKETIPNPEFLPKNEQQIAKDACKYMNLKPDEPIEGQSIDVCFIGSCTNGRLSDLQEASK
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IVKGNTVADGIRAFVVPGSQKVAKEAKEKGLDKIFLKAGFEWREPGCSMCLAMNPDKLEG
HHCCCCHHCCCEEEEECCCHHHHHHHHHCCHHEEHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSAKGRTLLMSPAMVAAAAINGRVTDVRKFLQE
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA