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The map label for this gene is leuD [H]

Identifier: 124025046

GI number: 124025046

Start: 307138

End: 307758

Strand: Reverse

Name: leuD [H]

Synonym: NATL1_03331

Alternate gene names: 124025046

Gene position: 307758-307138 (Counterclockwise)

Preceding gene: 124025047

Following gene: 124025043

Centisome position: 16.5

GC content: 34.94

Gene sequence:

>621_bases
ATGAATCAAAAGTTCCCAAAAGGTGAAATCAAATCAATACAAGGCCGCAGCTTTACAATGAAAGGCGATGATATTGATAC
AGACAGAATAATTCCTGCGAGATTTCTCAAATGTGTAAGTTTTTCCGCTTTAGGAGAACAAGTCTTTGCGGATGATAGAA
AAGAACTAAAGGGTAATCACCCATTTGATCTTGATCAAAATAAAGGGGCAAATATTCTTGTTGTCAATGACAATTTTGGC
TGTGGCTCAAGTCGAGAACATGCACCTCAGGCTCTTATGAGATGGGGGATAAGATTAATTATTGGGGAAAGCTTTGCTGA
AATCTTCTTTGGGAATTGCCTTGCTCTCGGGATACCTTGCCTAACAGCCGCAAAAGAAGAAATAGTTAACTTGCAAAACC
TAGTTGAAAAAAATAACAACCAAATTTGGGATTTCAATCTTAAAAACCTATCCATTTCTAATCAAAAAGAAACTGTCAAA
CTTTTTCTTGATGAAGGCCCCTACAATATGCTTTTCTCAGGAAAATGGGATGCTACCTCTCAACTACTAGATGAAGAAGA
AAAAATAAATAAAACATTTAATAACTTACCTTATCTCAATAATTTCAAACAAAAAATCTAA

Upstream 100 bases:

>100_bases
AATGAGCCCCGCTATGGTTGCTGCTGCTGCAATAAATGGGAGGGTTACAGATGTAAGAAAGTTCCTGCAAGAGTAAAACT
AAAAATAAACTTATTGAAAG

Downstream 100 bases:

>100_bases
AATACTGCTTTAGTAGAATTACCAAATTTAAATCCATTTATACGAAAATATAATCCTCCAGCATCATTATTAGGATGATA
GTAAATCCCACATTCGTATG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNQKFPKGEIKSIQGRSFTMKGDDIDTDRIIPARFLKCVSFSALGEQVFADDRKELKGNHPFDLDQNKGANILVVNDNFG
CGSSREHAPQALMRWGIRLIIGESFAEIFFGNCLALGIPCLTAAKEEIVNLQNLVEKNNNQIWDFNLKNLSISNQKETVK
LFLDEGPYNMLFSGKWDATSQLLDEEEKINKTFNNLPYLNNFKQKI

Sequences:

>Translated_206_residues
MNQKFPKGEIKSIQGRSFTMKGDDIDTDRIIPARFLKCVSFSALGEQVFADDRKELKGNHPFDLDQNKGANILVVNDNFG
CGSSREHAPQALMRWGIRLIIGESFAEIFFGNCLALGIPCLTAAKEEIVNLQNLVEKNNNQIWDFNLKNLSISNQKETVK
LFLDEGPYNMLFSGKWDATSQLLDEEEKINKTFNNLPYLNNFKQKI
>Mature_206_residues
MNQKFPKGEIKSIQGRSFTMKGDDIDTDRIIPARFLKCVSFSALGEQVFADDRKELKGNHPFDLDQNKGANILVVNDNFG
CGSSREHAPQALMRWGIRLIIGESFAEIFFGNCLALGIPCLTAAKEEIVNLQNLVEKNNNQIWDFNLKNLSISNQKETVK
LFLDEGPYNMLFSGKWDATSQLLDEEEKINKTFNNLPYLNNFKQKI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=156, Percent_Identity=34.6153846153846, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=115, Percent_Identity=38.2608695652174, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23410; Mature: 23410

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKFPKGEIKSIQGRSFTMKGDDIDTDRIIPARFLKCVSFSALGEQVFADDRKELKGNH
CCCCCCCCCHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
PFDLDQNKGANILVVNDNFGCGSSREHAPQALMRWGIRLIIGESFAEIFFGNCLALGIPC
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHEEECHHHHHHHHHHHHHHHHHH
LTAAKEEIVNLQNLVEKNNNQIWDFNLKNLSISNQKETVKLFLDEGPYNMLFSGKWDATS
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCCCCEEEEECCCCHHH
QLLDEEEKINKTFNNLPYLNNFKQKI
HHHHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure
MNQKFPKGEIKSIQGRSFTMKGDDIDTDRIIPARFLKCVSFSALGEQVFADDRKELKGNH
CCCCCCCCCHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
PFDLDQNKGANILVVNDNFGCGSSREHAPQALMRWGIRLIIGESFAEIFFGNCLALGIPC
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHEEECHHHHHHHHHHHHHHHHHH
LTAAKEEIVNLQNLVEKNNNQIWDFNLKNLSISNQKETVKLFLDEGPYNMLFSGKWDATS
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCCCCEEEEECCCCHHH
QLLDEEEKINKTFNNLPYLNNFKQKI
HHHHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]