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The map label for this gene is eno

Identifier: 124024997

GI number: 124024997

Start: 263364

End: 264665

Strand: Direct

Name: eno

Synonym: NATL1_02841

Alternate gene names: 124024997

Gene position: 263364-264665 (Clockwise)

Preceding gene: 124024986

Following gene: 124025000

Centisome position: 14.12

GC content: 40.63

Gene sequence:

>1302_bases
GTGGCTGACTCTCTAGAACTTGTAATTGATACCATTATGGCCCGAGAAGTGTTGGACTCACGTGGTAATCCAACAGTTGA
GGCTGAAGTGCTTTTAGAAGGTGGCGCAATAGGACGTTCAATTGTCCCTAGTGGCGCAAGCACTGGAGCTCATGAAGCTC
ATGAATTAAGAGATGGTGGTAAGCGTTATCTGGGTAAAGGAGTCCTTAAGGCCGTTAATCACATAGAAGAAAATATTGCT
CCCGCGCTATGCGGTCTGTCCTCTTTAGATCAAGCCACAGTTGATTCTGTAATGAAACAACTTGATGATACTGACAACAA
ATCAAATCTTGGAGCCAATTCAATTCTTGCTGTCAGTATGGCTACTGCAAGAGCCGCTGCAAATGGATTGGGATTACCTC
TGTATAGGTACTTAGGAGGGCCAATGTCATCATTATTACCAGTTCCATTGATGAATGTCATTAATGGAGGAGAACATGCA
GCAAATAATTTAGATTTTCAAGAATTTATGTTGGTTCCTCATGGCGCTGAAAGCTTCAGAGAAGCATTGAGAATGGGAGC
TGAGGTCTTTCATACGCTTAAAGATTTATTGAGTCAGAAAGGTTTATCAACTGCTGTTGGTGATGAAGGGGGATTCGCTC
CAAATCTTGAGAGTAATAAAGCAGCAGGCGATCTATTAATGCAAGCAATTGAACAGGCCGGATTTAAACCAGGAGAGCAA
GTATCTCTAGCTTTAGATGTTGCGAGTACGGAGTTTTATGAGGAGGGACAATATTGTTATGGTGGAAATTCTTATTCCAG
CGAGCAAATGGTTGAGGAATTGGCTGGATTAGTTAATTCATTTCCAATTGTTTCTATTGAAGATGGATTAGCTGAAGATG
ATTGGGATGGTTGGAGCTTGCTTACGAAAAAGCTTGGCAAAAGTGTGCAATTAGTTGGAGATGATTTATTTGTTACCAAC
ACACTGCGTTTGCAACGAGGAATTGATGAAAATATTGCAAATTCAATATTAATTAAGGTGAATCAAATAGGTTCTCTGAC
TGAAACCCTTGAAGCTATAGAGCTTGCCTCAAGATCAAGTTATACAACTGTTATCAGTCATAGAAGCGGAGAAACTGAAG
ATACAACTATTGCTGATTTATCAGTAGCAACTAAATCTGGTCAAATAAAAACAGGTTCATTGAGTCGGAGTGAAAGAGTC
GCGAAATATAATCAACTACTGCGTATTGAGGATGAGTTAGGAAGTCAAGCAACATATGCTGGGCTTGTTGGTTTGGGACC
AAGAGGAAGCTTGAAAGGGTGA

Upstream 100 bases:

>100_bases
CAAATTTGATTGATTAGGTCTTGGAAAGCAAGCTAGATTACCAATTAAGGAACATATAGGAATAATGTAGTTCATAAGAT
CTAAAAATCATCTTAAATTC

Downstream 100 bases:

>100_bases
TTTCAATAAACTAGCGAGAGCCTCGTCTTCCTTGAAAATTATCAAGTCGATTTTCGTTTCTCATTTTGAATTGTAATGTG
ATCCAATTTAGGGCTATTGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MADSLELVIDTIMAREVLDSRGNPTVEAEVLLEGGAIGRSIVPSGASTGAHEAHELRDGGKRYLGKGVLKAVNHIEENIA
PALCGLSSLDQATVDSVMKQLDDTDNKSNLGANSILAVSMATARAAANGLGLPLYRYLGGPMSSLLPVPLMNVINGGEHA
ANNLDFQEFMLVPHGAESFREALRMGAEVFHTLKDLLSQKGLSTAVGDEGGFAPNLESNKAAGDLLMQAIEQAGFKPGEQ
VSLALDVASTEFYEEGQYCYGGNSYSSEQMVEELAGLVNSFPIVSIEDGLAEDDWDGWSLLTKKLGKSVQLVGDDLFVTN
TLRLQRGIDENIANSILIKVNQIGSLTETLEAIELASRSSYTTVISHRSGETEDTTIADLSVATKSGQIKTGSLSRSERV
AKYNQLLRIEDELGSQATYAGLVGLGPRGSLKG

Sequences:

>Translated_433_residues
MADSLELVIDTIMAREVLDSRGNPTVEAEVLLEGGAIGRSIVPSGASTGAHEAHELRDGGKRYLGKGVLKAVNHIEENIA
PALCGLSSLDQATVDSVMKQLDDTDNKSNLGANSILAVSMATARAAANGLGLPLYRYLGGPMSSLLPVPLMNVINGGEHA
ANNLDFQEFMLVPHGAESFREALRMGAEVFHTLKDLLSQKGLSTAVGDEGGFAPNLESNKAAGDLLMQAIEQAGFKPGEQ
VSLALDVASTEFYEEGQYCYGGNSYSSEQMVEELAGLVNSFPIVSIEDGLAEDDWDGWSLLTKKLGKSVQLVGDDLFVTN
TLRLQRGIDENIANSILIKVNQIGSLTETLEAIELASRSSYTTVISHRSGETEDTTIADLSVATKSGQIKTGSLSRSERV
AKYNQLLRIEDELGSQATYAGLVGLGPRGSLKG
>Mature_432_residues
ADSLELVIDTIMAREVLDSRGNPTVEAEVLLEGGAIGRSIVPSGASTGAHEAHELRDGGKRYLGKGVLKAVNHIEENIAP
ALCGLSSLDQATVDSVMKQLDDTDNKSNLGANSILAVSMATARAAANGLGLPLYRYLGGPMSSLLPVPLMNVINGGEHAA
NNLDFQEFMLVPHGAESFREALRMGAEVFHTLKDLLSQKGLSTAVGDEGGFAPNLESNKAAGDLLMQAIEQAGFKPGEQV
SLALDVASTEFYEEGQYCYGGNSYSSEQMVEELAGLVNSFPIVSIEDGLAEDDWDGWSLLTKKLGKSVQLVGDDLFVTNT
LRLQRGIDENIANSILIKVNQIGSLTETLEAIELASRSSYTTVISHRSGETEDTTIADLSVATKSGQIKTGSLSRSERVA
KYNQLLRIEDELGSQATYAGLVGLGPRGSLKG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.4651162790698, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=438, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=438, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=436, Percent_Identity=44.954128440367, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=24.4117647058824, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=24.4117647058824, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=24.4117647058824, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=58.9073634204276, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.7777777777778, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=52.9002320185615, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=49.4736842105263, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=47.8060046189376, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=47.8060046189376, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=47.8060046189376, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=48.729792147806, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.4988452655889, Blast_Score=356, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=49.8839907192575, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=49.8839907192575, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PROM1 (A2C038)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014113.1
- ProteinModelPortal:   A2C038
- SMR:   A2C038
- STRING:   A2C038
- GeneID:   4779151
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02841
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   PMAR167555:NATL1_02841-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45932; Mature: 45801

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 339-339 BINDING 159-159 BINDING 168-168 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSLELVIDTIMAREVLDSRGNPTVEAEVLLEGGAIGRSIVPSGASTGAHEAHELRDGG
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
KRYLGKGVLKAVNHIEENIAPALCGLSSLDQATVDSVMKQLDDTDNKSNLGANSILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMSSLLPVPLMNVINGGEHAANNLDFQEFMLVPHGAESFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHEECCCCHHHHH
EALRMGAEVFHTLKDLLSQKGLSTAVGDEGGFAPNLESNKAAGDLLMQAIEQAGFKPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VSLALDVASTEFYEEGQYCYGGNSYSSEQMVEELAGLVNSFPIVSIEDGLAEDDWDGWSL
EEEEEEEHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LTKKLGKSVQLVGDDLFVTNTLRLQRGIDENIANSILIKVNQIGSLTETLEAIELASRSS
HHHHHCCCEEEECCCEEEEHHHHHHHCCCHHHHHHEEEEEHHHCCHHHHHHHHHHHCCCC
YTTVISHRSGETEDTTIADLSVATKSGQIKTGSLSRSERVAKYNQLLRIEDELGSQATYA
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
GLVGLGPRGSLKG
HEEECCCCCCCCC
>Mature Secondary Structure 
ADSLELVIDTIMAREVLDSRGNPTVEAEVLLEGGAIGRSIVPSGASTGAHEAHELRDGG
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
KRYLGKGVLKAVNHIEENIAPALCGLSSLDQATVDSVMKQLDDTDNKSNLGANSILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMSSLLPVPLMNVINGGEHAANNLDFQEFMLVPHGAESFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHEECCCCHHHHH
EALRMGAEVFHTLKDLLSQKGLSTAVGDEGGFAPNLESNKAAGDLLMQAIEQAGFKPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VSLALDVASTEFYEEGQYCYGGNSYSSEQMVEELAGLVNSFPIVSIEDGLAEDDWDGWSL
EEEEEEEHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LTKKLGKSVQLVGDDLFVTNTLRLQRGIDENIANSILIKVNQIGSLTETLEAIELASRSS
HHHHHCCCEEEECCCEEEEHHHHHHHCCCHHHHHHEEEEEHHHCCHHHHHHHHHHHCCCC
YTTVISHRSGETEDTTIADLSVATKSGQIKTGSLSRSERVAKYNQLLRIEDELGSQATYA
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
GLVGLGPRGSLKG
HEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA