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The map label for this gene is rnhA

Identifier: 124024989

GI number: 124024989

Start: 256326

End: 256811

Strand: Reverse

Name: rnhA

Synonym: NATL1_02761

Alternate gene names: 124024989

Gene position: 256811-256326 (Counterclockwise)

Preceding gene: 124024990

Following gene: 124024988

Centisome position: 13.77

GC content: 38.27

Gene sequence:

>486_bases
ATGTCTAAAGAAGAAAAATTAGCGATTGCTGCAGCAACCGATGGTGCATGTAGTGGTAATCCTGGTCCAGGTGGCTGGGG
AGCATTAATCAGATTCCAGGATGGAAGCGAAATTGAATTCGGTGGGAACTCCCCCGAGACAACGAATAATCGCATGGAAT
TACAGGCTGCATTATTTATTCTAGAAAAATTAAAAAACATAGAATTTGCCCCTTCACTAACTATTAAAACTGATAGCAAA
TATTTAATTGATGGAATGGAAAAATGGATGCCAAATTGGAAAAAGAAAGGCTGGAAAACTGCATCTGGCAAACCAGTTCT
CAATCAAGATTTATGGAAAGCTCTTGACCATCCTGAGCTACCAAAAATCAAACTTCAATATGTCAAAGGTCATAGCGGAG
AAAAGGATAATGACAGAGTCGATGCAATTGCGGTAGCTTTTTCTAAAGGTAGGAAAATACAACTTAAAGATTTTGCCAAC
AACTAA

Upstream 100 bases:

>100_bases
GAGGCTGTTGGCGGAAAAGTAACTTTAAAATAAATATCGTTACTGGAAAAACACCTGTTTTCTGCTTATATAAAACCGGT
CTATTCAGACCGGTTTTTTT

Downstream 100 bases:

>100_bases
AAAAAACGACTTATACAACATTCTCCTCGGTCAGCTTCTATCTCAAGACGAGGGTATTGATGCAGAAATACTTACTAATT
CAGCCCTTAATGCAATTAAA

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSKEEKLAIAAATDGACSGNPGPGGWGALIRFQDGSEIEFGGNSPETTNNRMELQAALFILEKLKNIEFAPSLTIKTDSK
YLIDGMEKWMPNWKKKGWKTASGKPVLNQDLWKALDHPELPKIKLQYVKGHSGEKDNDRVDAIAVAFSKGRKIQLKDFAN
N

Sequences:

>Translated_161_residues
MSKEEKLAIAAATDGACSGNPGPGGWGALIRFQDGSEIEFGGNSPETTNNRMELQAALFILEKLKNIEFAPSLTIKTDSK
YLIDGMEKWMPNWKKKGWKTASGKPVLNQDLWKALDHPELPKIKLQYVKGHSGEKDNDRVDAIAVAFSKGRKIQLKDFAN
N
>Mature_160_residues
SKEEKLAIAAATDGACSGNPGPGGWGALIRFQDGSEIEFGGNSPETTNNRMELQAALFILEKLKNIEFAPSLTIKTDSKY
LIDGMEKWMPNWKKKGWKTASGKPVLNQDLWKALDHPELPKIKLQYVKGHSGEKDNDRVDAIAVAFSKGRKIQLKDFANN

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=35.2112676056338, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1786408, Length=135, Percent_Identity=49.6296296296296, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532542, Length=137, Percent_Identity=41.6058394160584, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17505482, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552509, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17137476, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_PROM1 (A2C030)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014105.1
- ProteinModelPortal:   A2C030
- STRING:   A2C030
- GeneID:   4779952
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02761
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   CLSK919121
- BioCyc:   PMAR167555:NATL1_02761-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17803; Mature: 17672

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEEKLAIAAATDGACSGNPGPGGWGALIRFQDGSEIEFGGNSPETTNNRMELQAALFI
CCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHH
LEKLKNIEFAPSLTIKTDSKYLIDGMEKWMPNWKKKGWKTASGKPVLNQDLWKALDHPEL
HHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCC
PKIKLQYVKGHSGEKDNDRVDAIAVAFSKGRKIQLKDFANN
CCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCC
>Mature Secondary Structure 
SKEEKLAIAAATDGACSGNPGPGGWGALIRFQDGSEIEFGGNSPETTNNRMELQAALFI
CCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHH
LEKLKNIEFAPSLTIKTDSKYLIDGMEKWMPNWKKKGWKTASGKPVLNQDLWKALDHPEL
HHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCC
PKIKLQYVKGHSGEKDNDRVDAIAVAFSKGRKIQLKDFANN
CCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA