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The map label for this gene is pgk

Identifier: 124024984

GI number: 124024984

Start: 250960

End: 252165

Strand: Direct

Name: pgk

Synonym: NATL1_02711

Alternate gene names: 124024984

Gene position: 250960-252165 (Clockwise)

Preceding gene: 124024981

Following gene: 124024986

Centisome position: 13.46

GC content: 39.72

Gene sequence:

>1206_bases
ATGTCTAAGCGTTCCCTGTCAAGTCTCGGCGCTGAAGACTTATGCGGCAAACGTGTTTTAGTGAGAGTTGATTTTAATGT
CCCATTAGGAGATGAGGGGCAAATAACTGATGACACAAGAATTCGTGCTGCTTTACCAACTATTAATGACCTCTTGGATA
AGAGTGCAAGAGTCATTCTTTCTGCTCATTTTGGGAGGCCTAAGGGAGAGGTTAATGAAACAATGCGTTTGACTGCTGTT
GCTCAAAGACTTAGTGAATTGCTAGGAAAAACAGTTGTAAAGACTGAAAGTTGCATAGGAGCAGAAGCCAAGTCAAAAGT
TGATGCTATGTCTAATGGCGATGTTGTCCTTTTGGAAAATGTTCGATTTATTTCTGGTGAAGAAAAAAACGATACTGAAT
TTGCAAAAGAATTAGCTTCTCTGGCAGAAGTTTATGTTAATGATGCTTTTGGAGCCGCCCATCGTGCGCATGCTTCAACT
GAAGGAGTTACAAAGTTCTTAAGTCCTTGCGTGGCTGGCTATTTAATGGAAAAAGAACTGAAGTACCTTCAAGGAGCTAT
CGATCAACCTAAAAGTCCCCTAGCAGCTATAGTTGGAGGTTCAAAAGTTAGTAGTAAGATAGGTGTCTTAGAATCTTTAA
TTGATAAATGCGACAAGATAATTGTTGGTGGAGGGATGATATTTACTTTTTATAAGGCTAGAGGATTATCGGTTGGTAAT
AGTCTTGTAGAAGAAGATAAGCTCGAATTAGCTAGTGCTTTGGAGAAGAAAGCAAAAGAGAAAGGAGTTGAGTTTCTTTT
GCCAACTGATGTTGTGTTGGCTGATAATTTTTCTCCTGATGCAAATAGTAAACTTTCAAAAGTAGATGCTATTAGCGAAG
GTTGGATGGGATTGGATATTGGTTCAGAATCAGTTGAACTTTTTCAGAATGCTCTTAAAGATTGCAAAACGGTCATTTGG
AATGGACCGATGGGTGTCTTTGAATTTGAGAAATTTGCAAACGGAACAAATGCCATAGCAAATACTTTGGCTGAATTAAG
TGCTCAAGGATGTTGCACAATTATTGGAGGTGGAGATTCAGTGGCAGCAGTTGAGAAAGCAGGTTTAGCGAAAAACATGT
CTCATATCTCGACTGGGGGTGGTGCAAGCCTAGAACTTTTGGAAGGGAAAGTTCTTCCTGGAGTTTCTGCTCTAGATGAT
GCTTGA

Upstream 100 bases:

>100_bases
TCAAGGGGTTAGGTAGATTTTTTCTATGTCTTAGTAATTACCCAATAGGTGCCTTTAAAGAGCTATGGTCACGCAGTTTA
CTTTCTGAATCCCATACATT

Downstream 100 bases:

>100_bases
TAATTTAAATATAATTTGCTAATTGTTAAAAGTAGGTTTTATAAACCTACTTTTATTTTTTTTGTAAAGGAATAAATCCC
TTCTGGATGAGTAATTATTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSKRSLSSLGAEDLCGKRVLVRVDFNVPLGDEGQITDDTRIRAALPTINDLLDKSARVILSAHFGRPKGEVNETMRLTAV
AQRLSELLGKTVVKTESCIGAEAKSKVDAMSNGDVVLLENVRFISGEEKNDTEFAKELASLAEVYVNDAFGAAHRAHAST
EGVTKFLSPCVAGYLMEKELKYLQGAIDQPKSPLAAIVGGSKVSSKIGVLESLIDKCDKIIVGGGMIFTFYKARGLSVGN
SLVEEDKLELASALEKKAKEKGVEFLLPTDVVLADNFSPDANSKLSKVDAISEGWMGLDIGSESVELFQNALKDCKTVIW
NGPMGVFEFEKFANGTNAIANTLAELSAQGCCTIIGGGDSVAAVEKAGLAKNMSHISTGGGASLELLEGKVLPGVSALDD
A

Sequences:

>Translated_401_residues
MSKRSLSSLGAEDLCGKRVLVRVDFNVPLGDEGQITDDTRIRAALPTINDLLDKSARVILSAHFGRPKGEVNETMRLTAV
AQRLSELLGKTVVKTESCIGAEAKSKVDAMSNGDVVLLENVRFISGEEKNDTEFAKELASLAEVYVNDAFGAAHRAHAST
EGVTKFLSPCVAGYLMEKELKYLQGAIDQPKSPLAAIVGGSKVSSKIGVLESLIDKCDKIIVGGGMIFTFYKARGLSVGN
SLVEEDKLELASALEKKAKEKGVEFLLPTDVVLADNFSPDANSKLSKVDAISEGWMGLDIGSESVELFQNALKDCKTVIW
NGPMGVFEFEKFANGTNAIANTLAELSAQGCCTIIGGGDSVAAVEKAGLAKNMSHISTGGGASLELLEGKVLPGVSALDD
A
>Mature_400_residues
SKRSLSSLGAEDLCGKRVLVRVDFNVPLGDEGQITDDTRIRAALPTINDLLDKSARVILSAHFGRPKGEVNETMRLTAVA
QRLSELLGKTVVKTESCIGAEAKSKVDAMSNGDVVLLENVRFISGEEKNDTEFAKELASLAEVYVNDAFGAAHRAHASTE
GVTKFLSPCVAGYLMEKELKYLQGAIDQPKSPLAAIVGGSKVSSKIGVLESLIDKCDKIIVGGGMIFTFYKARGLSVGNS
LVEEDKLELASALEKKAKEKGVEFLLPTDVVLADNFSPDANSKLSKVDAISEGWMGLDIGSESVELFQNALKDCKTVIWN
GPMGVFEFEKFANGTNAIANTLAELSAQGCCTIIGGGDSVAAVEKAGLAKNMSHISTGGGASLELLEGKVLPGVSALDDA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=43.1980906921241, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI31543397, Length=419, Percent_Identity=41.527446300716, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=44.7300771208226, Blast_Score=301, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=46.1916461916462, Blast_Score=353, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=48.4412470023981, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17136394, Length=407, Percent_Identity=45.945945945946, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=43.0992736077482, Blast_Score=315, Evalue=5e-86,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PROM1 (A2C025)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014100.1
- ProteinModelPortal:   A2C025
- SMR:   A2C025
- STRING:   A2C025
- GeneID:   4779905
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02711
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   PMAR167555:NATL1_02711-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42362; Mature: 42231

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 122-122 BINDING 155-155 BINDING 206-206 BINDING 297-297 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRSLSSLGAEDLCGKRVLVRVDFNVPLGDEGQITDDTRIRAALPTINDLLDKSARVIL
CCCHHHHHCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHEEE
SAHFGRPKGEVNETMRLTAVAQRLSELLGKTVVKTESCIGAEAKSKVDAMSNGDVVLLEN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEC
VRFISGEEKNDTEFAKELASLAEVYVNDAFGAAHRAHASTEGVTKFLSPCVAGYLMEKEL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KYLQGAIDQPKSPLAAIVGGSKVSSKIGVLESLIDKCDKIIVGGGMIFTFYKARGLSVGN
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHCCCCCH
SLVEEDKLELASALEKKAKEKGVEFLLPTDVVLADNFSPDANSKLSKVDAISEGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHCCCEEECC
GSESVELFQNALKDCKTVIWNGPMGVFEFEKFANGTNAIANTLAELSAQGCCTIIGGGDS
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
VAAVEKAGLAKNMSHISTGGGASLELLEGKVLPGVSALDDA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure 
SKRSLSSLGAEDLCGKRVLVRVDFNVPLGDEGQITDDTRIRAALPTINDLLDKSARVIL
CCHHHHHCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHEEE
SAHFGRPKGEVNETMRLTAVAQRLSELLGKTVVKTESCIGAEAKSKVDAMSNGDVVLLEN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEC
VRFISGEEKNDTEFAKELASLAEVYVNDAFGAAHRAHASTEGVTKFLSPCVAGYLMEKEL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KYLQGAIDQPKSPLAAIVGGSKVSSKIGVLESLIDKCDKIIVGGGMIFTFYKARGLSVGN
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHCCCCCH
SLVEEDKLELASALEKKAKEKGVEFLLPTDVVLADNFSPDANSKLSKVDAISEGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHCCCEEECC
GSESVELFQNALKDCKTVIWNGPMGVFEFEKFANGTNAIANTLAELSAQGCCTIIGGGDS
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
VAAVEKAGLAKNMSHISTGGGASLELLEGKVLPGVSALDDA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA