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The map label for this gene is mraW

Identifier: 124024959

GI number: 124024959

Start: 227002

End: 227916

Strand: Reverse

Name: mraW

Synonym: NATL1_02461

Alternate gene names: 124024959

Gene position: 227916-227002 (Counterclockwise)

Preceding gene: 124024962

Following gene: 124024957

Centisome position: 12.22

GC content: 36.28

Gene sequence:

>915_bases
ATGAAAGAAGGGCCAATTAGTTCAAGTTCTAACTTTAATCATGTCCCTGTAATGGGGAAAGAAATAATTCAATCACTAAA
GGAATTGCCAAGTGAATTGACAAAACAAGGATTAATTATTGACGCCACAATTGGAGGAGGGGGTCATTCAGCTCAAATAC
TAGAAAATTTCCCAGGAATCAAAATTATTGGGCTTGATCAAGACCCAATGGCAAGAGAAGCAGCATCAAAAAAATTAATA
AAATTCGGGACAAGAATAAAAATAATCTCTACGAACTTCGCAGACTTTTCACTTGATGAACAAGCCATTTGCGTCTTAGC
GGATCTTGGTGTTAGTAGCCATCAACTAGATGAGCCTTCACGAGGTTTTAGCTTTCGTTTAAATGGTCCAATAGATATGC
GTATGAATCCCAAAGAGGGTTCAAGTGCAGCCGAACTTATTGAAACTCTCTCTGAGCAAAATCTAGCTGATTTAATATAT
GAATTAGGCGAAGAAAAACGATCTAGGCGTATTGCAAGAAAAATAAAAAATGATCTAGCTGAAAATGGACCATACTCTGG
AACTCAAGACCTTTCTTATGCAATTGCAGGCTGCTTCCCCCCCAAGCAAAGATATGGTCGAATTCATCCCTCAACAAGAA
CTTTTCAGGCTTTAAGAATAGCTGTGAATAATGAATTAGGCTCTCTCGAAAGTTTACTTTTAAAAGCTCCAAACTGGTTG
TTAGAGAATGGATTATTTATGGTAATGAGTTTTCATTCACTAGAAGATAGAAGAGTAAAATCGAGCTTTAAGACAGACAA
TAGATTGAAAGTACTATCTAAAAAGCCTATCAGAGCAAGCCCAGAAGAGATAGAATTAAATCCAAGAAGTAAAAGTGCAA
AGTTAAGAATTTCTGCAAAGAAATTCTTAACTTAG

Upstream 100 bases:

>100_bases
GGATGATGTGGCCCAAAGTTGACCACCATTGGCTCCGTACGCGTTTCAAGCTGCGCCATTGGGCCAAAACAAAGCAATGT
CTAAATACTAGTGAAAACTT

Downstream 100 bases:

>100_bases
TCTTAAATTATTGTCTAAGATTAATCACTATATTCGTAATAATATTAATAGCCTTATGTATATCTTTCTCCGTAGTATTT
CTCCCAATACTCATCCTTAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKEGPISSSSNFNHVPVMGKEIIQSLKELPSELTKQGLIIDATIGGGGHSAQILENFPGIKIIGLDQDPMAREAASKKLI
KFGTRIKIISTNFADFSLDEQAICVLADLGVSSHQLDEPSRGFSFRLNGPIDMRMNPKEGSSAAELIETLSEQNLADLIY
ELGEEKRSRRIARKIKNDLAENGPYSGTQDLSYAIAGCFPPKQRYGRIHPSTRTFQALRIAVNNELGSLESLLLKAPNWL
LENGLFMVMSFHSLEDRRVKSSFKTDNRLKVLSKKPIRASPEEIELNPRSKSAKLRISAKKFLT

Sequences:

>Translated_304_residues
MKEGPISSSSNFNHVPVMGKEIIQSLKELPSELTKQGLIIDATIGGGGHSAQILENFPGIKIIGLDQDPMAREAASKKLI
KFGTRIKIISTNFADFSLDEQAICVLADLGVSSHQLDEPSRGFSFRLNGPIDMRMNPKEGSSAAELIETLSEQNLADLIY
ELGEEKRSRRIARKIKNDLAENGPYSGTQDLSYAIAGCFPPKQRYGRIHPSTRTFQALRIAVNNELGSLESLLLKAPNWL
LENGLFMVMSFHSLEDRRVKSSFKTDNRLKVLSKKPIRASPEEIELNPRSKSAKLRISAKKFLT
>Mature_304_residues
MKEGPISSSSNFNHVPVMGKEIIQSLKELPSELTKQGLIIDATIGGGGHSAQILENFPGIKIIGLDQDPMAREAASKKLI
KFGTRIKIISTNFADFSLDEQAICVLADLGVSSHQLDEPSRGFSFRLNGPIDMRMNPKEGSSAAELIETLSEQNLADLIY
ELGEEKRSRRIARKIKNDLAENGPYSGTQDLSYAIAGCFPPKQRYGRIHPSTRTFQALRIAVNNELGSLESLLLKAPNWL
LENGLFMVMSFHSLEDRRVKSSFKTDNRLKVLSKKPIRASPEEIELNPRSKSAKLRISAKKFLT

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=33.4310850439883, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI165377202, Length=173, Percent_Identity=35.2601156069364, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=37.4213836477987, Blast_Score=170, Evalue=1e-43,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=30.4477611940298, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=30.4477611940298, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PROM1 (A2C000)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014075.1
- ProteinModelPortal:   A2C000
- SMR:   A2C000
- STRING:   A2C000
- GeneID:   4779688
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02461
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   PMAR167555:NATL1_02461-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33708; Mature: 33708

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 93-93 BINDING 108-108 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEGPISSSSNFNHVPVMGKEIIQSLKELPSELTKQGLIIDATIGGGGHSAQILENFPGI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCE
KIIGLDQDPMAREAASKKLIKFGTRIKIISTNFADFSLDEQAICVLADLGVSSHQLDEPS
EEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RGFSFRLNGPIDMRMNPKEGSSAAELIETLSEQNLADLIYELGEEKRSRRIARKIKNDLA
CCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENGPYSGTQDLSYAIAGCFPPKQRYGRIHPSTRTFQALRIAVNNELGSLESLLLKAPNWL
HCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHCCHHH
LENGLFMVMSFHSLEDRRVKSSFKTDNRLKVLSKKPIRASPEEIELNPRSKSAKLRISAK
HHCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEEEHH
KFLT
HHCC
>Mature Secondary Structure
MKEGPISSSSNFNHVPVMGKEIIQSLKELPSELTKQGLIIDATIGGGGHSAQILENFPGI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCE
KIIGLDQDPMAREAASKKLIKFGTRIKIISTNFADFSLDEQAICVLADLGVSSHQLDEPS
EEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RGFSFRLNGPIDMRMNPKEGSSAAELIETLSEQNLADLIYELGEEKRSRRIARKIKNDLA
CCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENGPYSGTQDLSYAIAGCFPPKQRYGRIHPSTRTFQALRIAVNNELGSLESLLLKAPNWL
HCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHCCHHH
LENGLFMVMSFHSLEDRRVKSSFKTDNRLKVLSKKPIRASPEEIELNPRSKSAKLRISAK
HHCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEEEHH
KFLT
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA