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The map label for this gene is menE [H]

Identifier: 124024962

GI number: 124024962

Start: 229597

End: 230832

Strand: Reverse

Name: menE [H]

Synonym: NATL1_02491

Alternate gene names: 124024962

Gene position: 230832-229597 (Counterclockwise)

Preceding gene: 124024963

Following gene: 124024959

Centisome position: 12.38

GC content: 36.57

Gene sequence:

>1236_bases
ATGACCAAAATTGCAGTTGTAGAGTGTATTCCTGAAAAAAACCTGGACTGTTCAAAAGAAATTTTGAAGAATTTTGAAAA
GCATAACTGGGTATATCTAGCGCCTCCAAAAGATCAAACGAAAATCCCCACATCGTCAACCTTACCTGAAGGAGAAGGAA
TAATCATTTCAAGCGGGGGCAGCCTTGGAGGGCCAAATCTTTGTTTCCAATCACTTAAAAATCTCACTAATTCGGCTTTA
GCAACCGGAAAGTGGTTAAAGAATCATGGCCTTAAGCCAAATGAATGCATCATTCTCAATTCACTACCTTTGCATCATAT
AAGTGGTTTCATGCCTTGGTGGAGACATCAGACTTGGGGATCTGGGTATTACTGGATTTCAAATTCTATAATGCATAAAC
CACTTCAACTTAAGCAATTTAGTGAAGCATTAACTAATAAACATAGACGTCCATTAATTACATCCCTAGTTCCAACTCAA
TTATTATCTTTAATTGATGATCCTGATGGTTTAGAATGGCTTCAATCTCTAGCTATAATCTGGGTTGGAGGATCCATGCT
TCCGACAGATCTTGCTGACAAAGCGCGAAGAAAGTCTATTAAATTAGCTCCTTGTTATGGGAGCACCGAAACAGTAGCAA
TGGTGACTTGCCTGAGTCCCCAAGATTTTTTAAAAGGAAGTAATAGTGTTGGGTTTCCTCTCGAAGATGTGGAGATAGAA
ATCAACAAAAGAAATTCACTTAAGATTAAAACTAGTAGGATTGCAACTTCAAAATGGAAGAATGATAAATTCGAATCAAT
TGCAGAATCAAATGGATGGTGGGAAGCAGGCGATTTAGCGCAATACATCACTCTCGACAATAGAACAGCAATTCAAATCC
TAGGTAGAAGAGATTCAGCTATAAATTCAGGTGGTGAAACAATTTTCCCAGAAGATATCGAAATGGAATTAATGAAAATA
ATCTCAAAACATCAAATCCCAATTAAAGACATTTTTGTACTAGGAGTTAGTGACAAGAAGTGGGGACAACGTTTAATTGT
CTTAACTAAATTCAAAGAAAAAGGAATTCATAGATATCCAATTATCAAACTTCTAACCGATCTCATTAAGGACTGGCAAC
CATCCAAAAAACCACTAAAATGGTACGACTGTCCAAAACTTTCACGAAATATCAATAAAAAATGGGAAGTAAAAAAATGG
CAAGATTGGGTAGCCTTGAATAGTCCTATCAGCTAA

Upstream 100 bases:

>100_bases
AAGTCAAAGGGGGAAATCCTTGTTCACCTGGACTTGCACCTGGATGGTGCCCAAAAGGCCCACTCTTCAACAACAATCCG
AAATTAGTCTGGGAAGCCGT

Downstream 100 bases:

>100_bases
AAATTCAAGCCAGATTCATGAAGCCACAAATTTATTTGTTTAGATAATGCACATTTTTCTCTTGTAATCGGATTTATTGA
TACATGTTTTATATTTGTCG

Product: putative O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTKIAVVECIPEKNLDCSKEILKNFEKHNWVYLAPPKDQTKIPTSSTLPEGEGIIISSGGSLGGPNLCFQSLKNLTNSAL
ATGKWLKNHGLKPNECIILNSLPLHHISGFMPWWRHQTWGSGYYWISNSIMHKPLQLKQFSEALTNKHRRPLITSLVPTQ
LLSLIDDPDGLEWLQSLAIIWVGGSMLPTDLADKARRKSIKLAPCYGSTETVAMVTCLSPQDFLKGSNSVGFPLEDVEIE
INKRNSLKIKTSRIATSKWKNDKFESIAESNGWWEAGDLAQYITLDNRTAIQILGRRDSAINSGGETIFPEDIEMELMKI
ISKHQIPIKDIFVLGVSDKKWGQRLIVLTKFKEKGIHRYPIIKLLTDLIKDWQPSKKPLKWYDCPKLSRNINKKWEVKKW
QDWVALNSPIS

Sequences:

>Translated_411_residues
MTKIAVVECIPEKNLDCSKEILKNFEKHNWVYLAPPKDQTKIPTSSTLPEGEGIIISSGGSLGGPNLCFQSLKNLTNSAL
ATGKWLKNHGLKPNECIILNSLPLHHISGFMPWWRHQTWGSGYYWISNSIMHKPLQLKQFSEALTNKHRRPLITSLVPTQ
LLSLIDDPDGLEWLQSLAIIWVGGSMLPTDLADKARRKSIKLAPCYGSTETVAMVTCLSPQDFLKGSNSVGFPLEDVEIE
INKRNSLKIKTSRIATSKWKNDKFESIAESNGWWEAGDLAQYITLDNRTAIQILGRRDSAINSGGETIFPEDIEMELMKI
ISKHQIPIKDIFVLGVSDKKWGQRLIVLTKFKEKGIHRYPIIKLLTDLIKDWQPSKKPLKWYDCPKLSRNINKKWEVKKW
QDWVALNSPIS
>Mature_410_residues
TKIAVVECIPEKNLDCSKEILKNFEKHNWVYLAPPKDQTKIPTSSTLPEGEGIIISSGGSLGGPNLCFQSLKNLTNSALA
TGKWLKNHGLKPNECIILNSLPLHHISGFMPWWRHQTWGSGYYWISNSIMHKPLQLKQFSEALTNKHRRPLITSLVPTQL
LSLIDDPDGLEWLQSLAIIWVGGSMLPTDLADKARRKSIKLAPCYGSTETVAMVTCLSPQDFLKGSNSVGFPLEDVEIEI
NKRNSLKIKTSRIATSKWKNDKFESIAESNGWWEAGDLAQYITLDNRTAIQILGRRDSAINSGGETIFPEDIEMELMKII
SKHQIPIKDIFVLGVSDKKWGQRLIVLTKFKEKGIHRYPIIKLLTDLIKDWQPSKKPLKWYDCPKLSRNINKKWEVKKWQ
DWVALNSPIS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=307, Percent_Identity=27.3615635179153, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 46649; Mature: 46517

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAVVECIPEKNLDCSKEILKNFEKHNWVYLAPPKDQTKIPTSSTLPEGEGIIISSGG
CCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCC
SLGGPNLCFQSLKNLTNSALATGKWLKNHGLKPNECIILNSLPLHHISGFMPWWRHQTWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCHHHHCCCCC
SGYYWISNSIMHKPLQLKQFSEALTNKHRRPLITSLVPTQLLSLIDDPDGLEWLQSLAII
CEEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEE
WVGGSMLPTDLADKARRKSIKLAPCYGSTETVAMVTCLSPQDFLKGSNSVGFPLEDVEIE
EECCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEEEECCHHHHHCCCCCCCCCCCCEEEE
INKRNSLKIKTSRIATSKWKNDKFESIAESNGWWEAGDLAQYITLDNRTAIQILGRRDSA
EECCCCEEEEEHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCC
INSGGETIFPEDIEMELMKIISKHQIPIKDIFVLGVSDKKWGQRLIVLTKFKEKGIHRYP
CCCCCCEECCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEEEEEHHHCCCCCCH
IIKLLTDLIKDWQPSKKPLKWYDCPKLSRNINKKWEVKKWQDWVALNSPIS
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHEECCCCCC
>Mature Secondary Structure 
TKIAVVECIPEKNLDCSKEILKNFEKHNWVYLAPPKDQTKIPTSSTLPEGEGIIISSGG
CEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCC
SLGGPNLCFQSLKNLTNSALATGKWLKNHGLKPNECIILNSLPLHHISGFMPWWRHQTWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCHHHHCCCCC
SGYYWISNSIMHKPLQLKQFSEALTNKHRRPLITSLVPTQLLSLIDDPDGLEWLQSLAII
CEEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEE
WVGGSMLPTDLADKARRKSIKLAPCYGSTETVAMVTCLSPQDFLKGSNSVGFPLEDVEIE
EECCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEEEECCHHHHHCCCCCCCCCCCCEEEE
INKRNSLKIKTSRIATSKWKNDKFESIAESNGWWEAGDLAQYITLDNRTAIQILGRRDSA
EECCCCEEEEEHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCC
INSGGETIFPEDIEMELMKIISKHQIPIKDIFVLGVSDKKWGQRLIVLTKFKEKGIHRYP
CCCCCCEECCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEEEEEHHHCCCCCCH
IIKLLTDLIKDWQPSKKPLKWYDCPKLSRNINKKWEVKKWQDWVALNSPIS
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]