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The map label for this gene is ndhI

Identifier: 124024945

GI number: 124024945

Start: 214917

End: 215573

Strand: Reverse

Name: ndhI

Synonym: NATL1_02321

Alternate gene names: 124024945

Gene position: 215573-214917 (Counterclockwise)

Preceding gene: 124024946

Following gene: 124024944

Centisome position: 11.56

GC content: 38.2

Gene sequence:

>657_bases
ATGCTTGGTTTCCTTGAAAAAGTTGCCGACTACACTAAAGAAGCCGTTAGTGCAGCAAAATATTTAGTTGATGGGCTAGG
AGTTACGTTCGACCACATGCGTCGAAGGCCTGTAACGGTTCAATACCCTTACGAAAAACTAATACCCTCTGAGCGTTATA
GAGGGAGGATTCATTATGAATTTGATAAGTGCATTGCATGCGAGGTTTGCGTAAGGGTATGCCCAATCAATCTGCCAGTC
GTTGATTGGGTAATGAATAAAGAAACCAAGAAAAAAGAATTGAGAAATTATTCTATTGACTTTGGTGCATGTATTTTTTG
TGGAAATTGCGTTGAATATTGCCCTACAAATTGCTTATCTATGACAGAAGAATATGAACTGTCTGCATTTGATAGACATA
GCCTTAACTACGATAATGTTGCTCTTGGAAGACTACCAACAAGCGTAACTTCAGATCCATCAGTTCGTCCTCTAAGAGAA
TTACCCTATTTGCCAAAGGGCATAATGGACCCTCATGAATTACCCGCGAACCAACAAAGAGCAGGTAAGCTTCCAAGTCA
GATTATAAAAGAACTGCAAGCAGAGAAATCAGAAGAAAAAGGTAATAATAATTCGTCGGATATTGTTCCAAATAAATTAA
ATTCTACTAACAAATGA

Upstream 100 bases:

>100_bases
CAACACACTAGCTAGTTTTTTAAGTAAAAGTACCTACATTTAATTGAAAAAAACTGTATAATGATTACTGTAGACATAGA
CCGCCGGCATCGAATTTAAT

Downstream 100 bases:

>100_bases
ATATCGCTTATTCAACAGAATTAATTTGCTTTTTAATTCTTAGTGCCATCATAGTTTCTGGAAGTTTGGCAGTTGTTTTA
TTAGAAAATATTGTTTACTC

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MLGFLEKVADYTKEAVSAAKYLVDGLGVTFDHMRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGACIFCGNCVEYCPTNCLSMTEEYELSAFDRHSLNYDNVALGRLPTSVTSDPSVRPLRE
LPYLPKGIMDPHELPANQQRAGKLPSQIIKELQAEKSEEKGNNNSSDIVPNKLNSTNK

Sequences:

>Translated_218_residues
MLGFLEKVADYTKEAVSAAKYLVDGLGVTFDHMRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGACIFCGNCVEYCPTNCLSMTEEYELSAFDRHSLNYDNVALGRLPTSVTSDPSVRPLRE
LPYLPKGIMDPHELPANQQRAGKLPSQIIKELQAEKSEEKGNNNSSDIVPNKLNSTNK
>Mature_218_residues
MLGFLEKVADYTKEAVSAAKYLVDGLGVTFDHMRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGACIFCGNCVEYCPTNCLSMTEEYELSAFDRHSLNYDNVALGRLPTSVTSDPSVRPLRE
LPYLPKGIMDPHELPANQQRAGKLPSQIIKELQAEKSEEKGNNNSSDIVPNKLNSTNK

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=126, Percent_Identity=32.5396825396825, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788617, Length=117, Percent_Identity=32.4786324786325, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1789075, Length=98, Percent_Identity=32.6530612244898, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=126, Percent_Identity=31.7460317460317, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864306, Length=143, Percent_Identity=31.4685314685315, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHI_PROM1 (A2BZY6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014061.1
- ProteinModelPortal:   A2BZY6
- SMR:   A2BZY6
- STRING:   A2BZY6
- GeneID:   4779510
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02321
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   HYSIDFG
- ProtClustDB:   PRK05888
- BioCyc:   PMAR167555:NATL1_02321-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00403
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: 1.6.5.3

Molecular weight: Translated: 24830; Mature: 24830

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFLEKVADYTKEAVSAAKYLVDGLGVTFDHMRRRPVTVQYPYEKLIPSERYRGRIHYE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCHHHCCCCHHHCCCEEEH
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGACIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHH
MTEEYELSAFDRHSLNYDNVALGRLPTSVTSDPSVRPLRELPYLPKGIMDPHELPANQQR
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCHHH
AGKLPSQIIKELQAEKSEEKGNNNSSDIVPNKLNSTNK
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGFLEKVADYTKEAVSAAKYLVDGLGVTFDHMRRRPVTVQYPYEKLIPSERYRGRIHYE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCHHHCCCCHHHCCCEEEH
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGACIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHH
MTEEYELSAFDRHSLNYDNVALGRLPTSVTSDPSVRPLRELPYLPKGIMDPHELPANQQR
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCHHH
AGKLPSQIIKELQAEKSEEKGNNNSSDIVPNKLNSTNK
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA