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Identifier: 124024845

GI number: 124024845

Start: 128073

End: 128681

Strand: Reverse

Name: def

Synonym: NATL1_01321

Alternate gene names: 124024845

Gene position: 128681-128073 (Counterclockwise)

Preceding gene: 124024847

Following gene: 124024844

Centisome position: 6.9

GC content: 36.62

Gene sequence:

>609_bases
TTGGCTGGCAGTTTTGCTCAACTTGCAAAAAATGCAGAAAAAAAGAAGCCCTCAATTTCAGTTTCTAAAGAACCTGTCGA
GAATCCCCCTTTGAAGGTGTATCAGCTGGGAGACGAGGCTTTAAGAACTCCAGCTAATCGTATCGTCAAAGTTGATGATG
CAATTAGAAAGTTAGCGAAAGATATGCTTATAACTATGTATTCATCAAAAGGGATTGGTTTAGCAGCACCTCAAGTAGGA
ATACAAAAGAGATTGTTAGTTATTGATTTAAATTTTGAAGATCCAAATTCTCCACCGATGGTATTTATAAATCCTGAGAT
AATTTCATCTAGTGCAACCGTAGATACATATGAAGAAGGTTGTCTAAGTATCCCTGGAGTTTACCTGAATGTTTTACGAC
CCTCCTCAATCAAATTAAGCTATAGAGATGAAATGGGTAGACCAAAAAAAATGAATGCAGACGGTCTCATGGCAAGATGC
ATTCAGCACGAAATAGACCACTTAAATGGTGTCTGTTTTGTCGATAAAGTAACTGATGAAGAAGAGCTGAAAAAACAATT
AAATGAGAATAACTTCAAGCGAAGCGATGTAATAAAAGCAACGAATTGA

Upstream 100 bases:

>100_bases
CTCATTGTTTTGTTCTTCATTGATGTTGAGAGTCAATGATTAATGCACTCATTGAACTTAAAACAAAGTAAAGTATAAAA
GTTGTTGTCTTAAGACTCCT

Downstream 100 bases:

>100_bases
AAAAATCTTGTCAATTCAAGCTATAAAAAATCGCATTAGATTCTTCTTATTGCTCTCTACACAAATTCAATGACAACAAT
CTTCGATAAAATTCTTAGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAGSFAQLAKNAEKKKPSISVSKEPVENPPLKVYQLGDEALRTPANRIVKVDDAIRKLAKDMLITMYSSKGIGLAAPQVG
IQKRLLVIDLNFEDPNSPPMVFINPEIISSSATVDTYEEGCLSIPGVYLNVLRPSSIKLSYRDEMGRPKKMNADGLMARC
IQHEIDHLNGVCFVDKVTDEEELKKQLNENNFKRSDVIKATN

Sequences:

>Translated_202_residues
MAGSFAQLAKNAEKKKPSISVSKEPVENPPLKVYQLGDEALRTPANRIVKVDDAIRKLAKDMLITMYSSKGIGLAAPQVG
IQKRLLVIDLNFEDPNSPPMVFINPEIISSSATVDTYEEGCLSIPGVYLNVLRPSSIKLSYRDEMGRPKKMNADGLMARC
IQHEIDHLNGVCFVDKVTDEEELKKQLNENNFKRSDVIKATN
>Mature_201_residues
AGSFAQLAKNAEKKKPSISVSKEPVENPPLKVYQLGDEALRTPANRIVKVDDAIRKLAKDMLITMYSSKGIGLAAPQVGI
QKRLLVIDLNFEDPNSPPMVFINPEIISSSATVDTYEEGCLSIPGVYLNVLRPSSIKLSYRDEMGRPKKMNADGLMARCI
QHEIDHLNGVCFVDKVTDEEELKKQLNENNFKRSDVIKATN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=31.7647058823529, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=40.5882352941176, Blast_Score=119, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=31.7365269461078, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24645726, Length=167, Percent_Identity=28.7425149700599, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PROM1 (A2BZN6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001013961.1
- ProteinModelPortal:   A2BZN6
- SMR:   A2BZN6
- STRING:   A2BZN6
- GeneID:   4781113
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_01321
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   AADMAET
- ProtClustDB:   PRK00150
- BioCyc:   PMAR167555:NATL1_01321-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22431; Mature: 22300

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSFAQLAKNAEKKKPSISVSKEPVENPPLKVYQLGDEALRTPANRIVKVDDAIRKLAK
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHEEEHHHHHHHHHH
DMLITMYSSKGIGLAAPQVGIQKRLLVIDLNFEDPNSPPMVFINPEIISSSATVDTYEEG
HHHHHHCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCCCCCCHHHCC
CLSIPGVYLNVLRPSSIKLSYRDEMGRPKKMNADGLMARCIQHEIDHLNGVCFVDKVTDE
CCCCCCCEEEEECCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCH
EELKKQLNENNFKRSDVIKATN
HHHHHHHCCCCCCHHCCEECCC
>Mature Secondary Structure 
AGSFAQLAKNAEKKKPSISVSKEPVENPPLKVYQLGDEALRTPANRIVKVDDAIRKLAK
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHEEEHHHHHHHHHH
DMLITMYSSKGIGLAAPQVGIQKRLLVIDLNFEDPNSPPMVFINPEIISSSATVDTYEEG
HHHHHHCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCCCCCCHHHCC
CLSIPGVYLNVLRPSSIKLSYRDEMGRPKKMNADGLMARCIQHEIDHLNGVCFVDKVTDE
CCCCCCCEEEEECCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCH
EELKKQLNENNFKRSDVIKATN
HHHHHHHCCCCCCHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA