Click here to switch to the map view.

The map label for this gene is yfcG [H]

Identifier: 124024752

GI number: 124024752

Start: 41013

End: 41780

Strand: Reverse

Name: yfcG [H]

Synonym: NATL1_00391

Alternate gene names: 124024752

Gene position: 41780-41013 (Counterclockwise)

Preceding gene: 124024753

Following gene: 124024751

Centisome position: 2.24

GC content: 32.94

Gene sequence:

>768_bases
ATGAAAGAACCTGCAAAAAAAGTAGAAGATAGTTCTCAAAAAGATATACATCTTTATACTGCTCCAACTATGAATGGATG
GAAACCGATAATCTTTCTTGAAGAAGCAGGCATTGAATATGATCTTACTTATGTTGATTTTTCTAAGAACGAACAGAAGG
ATAAAAATTACATCAAAAATATAAATCCCAATGGAAGAATCCCTTCTATTATTGATCGTTCAAATAATGATTTCTGTGTT
TTCGAGAGTGGAGCAATATTATGGTATCTTGCCGAAAAATATAATAAGTTCTTACCGAGGAATGCCAATGAAAAATCTAT
AACAATGCAATGGTTGATGTTTCAAGTTGGTGGAATAGGTCCAATGATGGGTCAAGCGATGTATTTTCAAAGAATTGCTG
CTTTAAATGGCGAGAATAATCAGTTTTCTATAAAGCGTTATGTAGATGAAAGTCGTCGTTTATTTGAAGTGCTTGATAAA
CAACTCGCATCACATAAATTTATCGTCAGTAACGAATTTACAATTGCTGATATTGCTACTTATCCTTGGGCAAGAACTTA
TTATTGGGCAAATGTCCCTATTGATGGTCTACCTAATTTAAAAGCATGGTTTAATCGAATAGATAATAGAAAAAATACAC
AACGAGCACTTGAAATCCCACAACCTCGTTGGGCATTTTTTGGAAAGGGGGATATACAATCTCAAGAGAGTGAAAATGCA
GAAAACTTTAAAAGTGATGTCAAATCCGTATTAAATGGAAATAAATGA

Upstream 100 bases:

>100_bases
ACGAAGAATGTAAACATGACTATAAAGTGATGCATGAAACCGCTAAAAAACATGGCATAAAGAATCCAATTTTAGAAATA
CCAATGGAAGTCGATTTCTA

Downstream 100 bases:

>100_bases
AACGCTTAATACTTACATTACTTAAAAAATTAAATGGAAAAGAAACGCAATTACGATAATGATGAAGATACATATTTCCC
TCCAGAAGAATCAGAACCGC

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKEPAKKVEDSSQKDIHLYTAPTMNGWKPIIFLEEAGIEYDLTYVDFSKNEQKDKNYIKNINPNGRIPSIIDRSNNDFCV
FESGAILWYLAEKYNKFLPRNANEKSITMQWLMFQVGGIGPMMGQAMYFQRIAALNGENNQFSIKRYVDESRRLFEVLDK
QLASHKFIVSNEFTIADIATYPWARTYYWANVPIDGLPNLKAWFNRIDNRKNTQRALEIPQPRWAFFGKGDIQSQESENA
ENFKSDVKSVLNGNK

Sequences:

>Translated_255_residues
MKEPAKKVEDSSQKDIHLYTAPTMNGWKPIIFLEEAGIEYDLTYVDFSKNEQKDKNYIKNINPNGRIPSIIDRSNNDFCV
FESGAILWYLAEKYNKFLPRNANEKSITMQWLMFQVGGIGPMMGQAMYFQRIAALNGENNQFSIKRYVDESRRLFEVLDK
QLASHKFIVSNEFTIADIATYPWARTYYWANVPIDGLPNLKAWFNRIDNRKNTQRALEIPQPRWAFFGKGDIQSQESENA
ENFKSDVKSVLNGNK
>Mature_255_residues
MKEPAKKVEDSSQKDIHLYTAPTMNGWKPIIFLEEAGIEYDLTYVDFSKNEQKDKNYIKNINPNGRIPSIIDRSNNDFCV
FESGAILWYLAEKYNKFLPRNANEKSITMQWLMFQVGGIGPMMGQAMYFQRIAALNGENNQFSIKRYVDESRRLFEVLDK
QLASHKFIVSNEFTIADIATYPWARTYYWANVPIDGLPNLKAWFNRIDNRKNTQRALEIPQPRWAFFGKGDIQSQESENA
ENFKSDVKSVLNGNK

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=41.2621359223301, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI87082195, Length=208, Percent_Identity=37.9807692307692, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87081789, Length=167, Percent_Identity=27.5449101796407, Blast_Score=61, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324100, Length=178, Percent_Identity=32.5842696629214, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=26.4367816091954, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17933730, Length=195, Percent_Identity=24.1025641025641, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549270, Length=193, Percent_Identity=24.3523316062176, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864592, Length=194, Percent_Identity=25.2577319587629, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 29635; Mature: 29635

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPAKKVEDSSQKDIHLYTAPTMNGWKPIIFLEEAGIEYDLTYVDFSKNEQKDKNYIKN
CCCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHC
INPNGRIPSIIDRSNNDFCVFESGAILWYLAEKYNKFLPRNANEKSITMQWLMFQVGGIG
CCCCCCCCHHEECCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
PMMGQAMYFQRIAALNGENNQFSIKRYVDESRRLFEVLDKQLASHKFIVSNEFTIADIAT
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEECCC
YPWARTYYWANVPIDGLPNLKAWFNRIDNRKNTQRALEIPQPRWAFFGKGDIQSQESENA
CCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCH
ENFKSDVKSVLNGNK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEPAKKVEDSSQKDIHLYTAPTMNGWKPIIFLEEAGIEYDLTYVDFSKNEQKDKNYIKN
CCCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHC
INPNGRIPSIIDRSNNDFCVFESGAILWYLAEKYNKFLPRNANEKSITMQWLMFQVGGIG
CCCCCCCCHHEECCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
PMMGQAMYFQRIAALNGENNQFSIKRYVDESRRLFEVLDKQLASHKFIVSNEFTIADIAT
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEECCC
YPWARTYYWANVPIDGLPNLKAWFNRIDNRKNTQRALEIPQPRWAFFGKGDIQSQESENA
CCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCH
ENFKSDVKSVLNGNK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]