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The map label for this gene is scrFIR [H]

Identifier: 124024751

GI number: 124024751

Start: 39959

End: 40753

Strand: Reverse

Name: scrFIR [H]

Synonym: NATL1_00381

Alternate gene names: 124024751

Gene position: 40753-39959 (Counterclockwise)

Preceding gene: 124024752

Following gene: 124024749

Centisome position: 2.19

GC content: 29.94

Gene sequence:

>795_bases
ATGGATGCAGTTAAAGAAAAAATAAAGGAATTATTTTTTAAAAATGTATATGGTTTATCACCGAATATTGAAGGTTATGA
CAAAAAGCATGCTGGAGCGAAAGGGCATTGGTTAGAAAAAAAACTTGGGAAGACACCAGATGCTTCAAATGAAGCAGATT
TTTGGGGTTACGAATGTAAAAACTTCACTAGCAACAAGACAACGTGGGGTGATTGGAGTGCTAATGAATATATTTTTGAT
AAAAATAATGAGTTTCAAATTCCCAGAGATAAATTTATTAAATTATTTGGCAAACCAAATCAAGCAAAAAAGAACAGATG
TTCATGGTCAGGACAACCAGTTCCAGATTCTCCTAATAAATACACTTTATTTGGACAAATAATGTCTATTGATGAATCTT
TGAATATCTCAATTACATATTCTTTTGAGAAGGATCAAAGAGATAACAAATTTGATTTGATGCCAAAAGAATTTCATACT
AATAATTTGCTTCTAGCAAAATGGTATGGATATGAAAGAAATAACAATTCCAAAAAAACTGCTCTCGAAACAAAAATCAG
CAAAAAGTTTAATCAAAAAGGTTGGTTTAAGTGTCTTATGAAAAATAATACTTATAATGAAATCGCTTTTGGTAAACCTA
TAAATTTTGAATTTTGGATGAATTATGTAGAGAAAGGTGATATTTTCTTCGATAGCGGCATGTACCAAGGAAATAATAGA
AATTATTCACAGTGGAGATCTATTAACTCATTTTGGGAAGAACTAATCGAGGAAAGATACACTAAAGCAATTTAA

Upstream 100 bases:

>100_bases
ACCCAATTTTTTCTAATTCACTAGAACTGGCAATCTCTAATTTGATTTGTCTTTGTAATTAAATTCCGCTACTGTAATTT
TCATTACTTGAAAAGTATGT

Downstream 100 bases:

>100_bases
CTATTCTCTCTGCAATAGCGCGAATTACTGGCACAGATACTGAATTACCAAATTGCTTATATGCCTGCAAATTACTAACG
GGAATCTTAAAACCTTCAGG

Product: hypothetical protein

Products: NA

Alternate protein names: R.ScrFI; Endonuclease ScrFI; Type II restriction enzyme ScrFI [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDAVKEKIKELFFKNVYGLSPNIEGYDKKHAGAKGHWLEKKLGKTPDASNEADFWGYECKNFTSNKTTWGDWSANEYIFD
KNNEFQIPRDKFIKLFGKPNQAKKNRCSWSGQPVPDSPNKYTLFGQIMSIDESLNISITYSFEKDQRDNKFDLMPKEFHT
NNLLLAKWYGYERNNNSKKTALETKISKKFNQKGWFKCLMKNNTYNEIAFGKPINFEFWMNYVEKGDIFFDSGMYQGNNR
NYSQWRSINSFWEELIEERYTKAI

Sequences:

>Translated_264_residues
MDAVKEKIKELFFKNVYGLSPNIEGYDKKHAGAKGHWLEKKLGKTPDASNEADFWGYECKNFTSNKTTWGDWSANEYIFD
KNNEFQIPRDKFIKLFGKPNQAKKNRCSWSGQPVPDSPNKYTLFGQIMSIDESLNISITYSFEKDQRDNKFDLMPKEFHT
NNLLLAKWYGYERNNNSKKTALETKISKKFNQKGWFKCLMKNNTYNEIAFGKPINFEFWMNYVEKGDIFFDSGMYQGNNR
NYSQWRSINSFWEELIEERYTKAI
>Mature_264_residues
MDAVKEKIKELFFKNVYGLSPNIEGYDKKHAGAKGHWLEKKLGKTPDASNEADFWGYECKNFTSNKTTWGDWSANEYIFD
KNNEFQIPRDKFIKLFGKPNQAKKNRCSWSGQPVPDSPNKYTLFGQIMSIDESLNISITYSFEKDQRDNKFDLMPKEFHT
NNLLLAKWYGYERNNNSKKTALETKISKKFNQKGWFKCLMKNNTYNEIAFGKPINFEFWMNYVEKGDIFFDSGMYQGNNR
NYSQWRSINSFWEELIEERYTKAI

Specific function: Recognizes the double-stranded sequence CCNGG and cleaves after C-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019063 [H]

Pfam domain/function: PF09562 RE_LlaMI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 31270; Mature: 31270

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVKEKIKELFFKNVYGLSPNIEGYDKKHAGAKGHWLEKKLGKTPDASNEADFWGYECK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
NFTSNKTTWGDWSANEYIFDKNNEFQIPRDKFIKLFGKPNQAKKNRCSWSGQPVPDSPNK
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
YTLFGQIMSIDESLNISITYSFEKDQRDNKFDLMPKEFHTNNLLLAKWYGYERNNNSKKT
EEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCEEEEEEECEECCCCCHHH
ALETKISKKFNQKGWFKCLMKNNTYNEIAFGKPINFEFWMNYVEKGDIFFDSGMYQGNNR
HHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCHHHHHHHHHCCCEEEECCEEECCCC
NYSQWRSINSFWEELIEERYTKAI
CHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAVKEKIKELFFKNVYGLSPNIEGYDKKHAGAKGHWLEKKLGKTPDASNEADFWGYECK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
NFTSNKTTWGDWSANEYIFDKNNEFQIPRDKFIKLFGKPNQAKKNRCSWSGQPVPDSPNK
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
YTLFGQIMSIDESLNISITYSFEKDQRDNKFDLMPKEFHTNNLLLAKWYGYERNNNSKKT
EEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCEEEEEEECEECCCCCHHH
ALETKISKKFNQKGWFKCLMKNNTYNEIAFGKPINFEFWMNYVEKGDIFFDSGMYQGNNR
HHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCHHHHHHHHHCCCEEEECCEEECCCC
NYSQWRSINSFWEELIEERYTKAI
CHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9245816 [H]