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The map label for this gene is grpE

Identifier: 124024728

GI number: 124024728

Start: 18732

End: 19511

Strand: Direct

Name: grpE

Synonym: NATL1_00151

Alternate gene names: 124024728

Gene position: 18732-19511 (Clockwise)

Preceding gene: 124024727

Following gene: 124024729

Centisome position: 1.0

GC content: 38.08

Gene sequence:

>780_bases
ATGAATTCAGACGTTTCCTCTTCCGAACATGAATTATCACAAGATGGTTCATCACAAAATAATCCCAGTGAAAATTTTGT
AAGTTCTTCTAATAGTAATGAATCAGTTAATCAAGTTGAGCTTTCTGATAATCCCGAAGTAGAGCATCAAGTGAAGAATG
ATTCAGTAGATACAGCAAAAGAACAATCTTCAACTAGTTGTGAGTCAAATATTAAAGGTTCAGATACTGAAGCAAGATTA
CAGCAATTAGAAAAAGAGCATGAAACTTTAAACAGCCAATATATGAGAATAGCTGCTGACTTTGATAATTTCCGAAAACG
ACAGACGCGTGATCAAGATGATCTAAAAATTCAACTTACTTGCACAACCCTTAGTGAAATTCTTCCTATTGTTGATAATT
TTGAAAGAGCAAGGCAGCAGTTGAATCCTGAGGGTGAAGAAGCTCAAGCATTACACCGCAGTTACCAAGGGCTTTACAAA
CAATTAGTTGAAGTTCTTAAGAATCTTGGCGTTGCTCCAATGCGAGTGGTTGATCAGGCTTTTGATCCGTCTTTGCATGA
GGCAGTTATGAGAGAGCCCAGCGATGAAAAGGCTGAGGATATTGTGATTGAAGAATTACAACGGGGCTATCACCTTAACG
GTCGTGTTTTAAGGCATGCCTTGGTTAAAGTTTCTATGGGTCCAGGCCCGAAAGCTGTTAATGAAGAGATTCCTGATCAG
AGTGCTTCTAATCAAGAACTAAGTGAATCTGTAGATGGTCCAACTAAAGATGAGAATTAA

Upstream 100 bases:

>100_bases
TCGTAAATGATTATCTTTTCTATTCAAATAGGTGTGGGCTTCCGTAGCTTGTACAAAAAACCCTTTGCAATCAACATGGT
CTAAAAGCTGATTGAATTAT

Downstream 100 bases:

>100_bases
CTACATGATGATCGAAACGGAATTGAATTTGTTTTAATGTACTAATGGCTGATTTTTACGATCTATTGGGTGTCAGCAGA
GATGCTGATGCTGACACTTT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNSDVSSSEHELSQDGSSQNNPSENFVSSSNSNESVNQVELSDNPEVEHQVKNDSVDTAKEQSSTSCESNIKGSDTEARL
QQLEKEHETLNSQYMRIAADFDNFRKRQTRDQDDLKIQLTCTTLSEILPIVDNFERARQQLNPEGEEAQALHRSYQGLYK
QLVEVLKNLGVAPMRVVDQAFDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQ
SASNQELSESVDGPTKDEN

Sequences:

>Translated_259_residues
MNSDVSSSEHELSQDGSSQNNPSENFVSSSNSNESVNQVELSDNPEVEHQVKNDSVDTAKEQSSTSCESNIKGSDTEARL
QQLEKEHETLNSQYMRIAADFDNFRKRQTRDQDDLKIQLTCTTLSEILPIVDNFERARQQLNPEGEEAQALHRSYQGLYK
QLVEVLKNLGVAPMRVVDQAFDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQ
SASNQELSESVDGPTKDEN
>Mature_259_residues
MNSDVSSSEHELSQDGSSQNNPSENFVSSSNSNESVNQVELSDNPEVEHQVKNDSVDTAKEQSSTSCESNIKGSDTEARL
QQLEKEHETLNSQYMRIAADFDNFRKRQTRDQDDLKIQLTCTTLSEILPIVDNFERARQQLNPEGEEAQALHRSYQGLYK
QLVEVLKNLGVAPMRVVDQAFDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQ
SASNQELSESVDGPTKDEN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=132, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PROM1 (A2BZB9)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001013844.1
- ProteinModelPortal:   A2BZB9
- SMR:   A2BZB9
- STRING:   A2BZB9
- GeneID:   4781281
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_00151
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14143
- BioCyc:   PMAR167555:NATL1_00151-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 29009; Mature: 29009

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDVSSSEHELSQDGSSQNNPSENFVSSSNSNESVNQVELSDNPEVEHQVKNDSVDTAK
CCCCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHCCCCHHHHH
EQSSTSCESNIKGSDTEARLQQLEKEHETLNSQYMRIAADFDNFRKRQTRDQDDLKIQLT
HHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
CTTLSEILPIVDNFERARQQLNPEGEEAQALHRSYQGLYKQLVEVLKNLGVAPMRVVDQA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQ
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCC
SASNQELSESVDGPTKDEN
CCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNSDVSSSEHELSQDGSSQNNPSENFVSSSNSNESVNQVELSDNPEVEHQVKNDSVDTAK
CCCCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHCCCCHHHHH
EQSSTSCESNIKGSDTEARLQQLEKEHETLNSQYMRIAADFDNFRKRQTRDQDDLKIQLT
HHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
CTTLSEILPIVDNFERARQQLNPEGEEAQALHRSYQGLYKQLVEVLKNLGVAPMRVVDQA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FDPSLHEAVMREPSDEKAEDIVIEELQRGYHLNGRVLRHALVKVSMGPGPKAVNEEIPDQ
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCC
SASNQELSESVDGPTKDEN
CCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA