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The map label for this gene is pcn [H]

Identifier: 124028484

GI number: 124028484

Start: 1641461

End: 1642201

Strand: Reverse

Name: pcn [H]

Synonym: Hbut_1645

Alternate gene names: 124028484

Gene position: 1642201-1641461 (Counterclockwise)

Preceding gene: 124028485

Following gene: 124028483

Centisome position: 98.5

GC content: 46.96

Gene sequence:

>741_bases
TTGAAGCTTGTATTCTTTGATGCGAGGATTTGGAGGTACATAGTGTCAGCTATATCGAAGGTTATAGAGGAGGGGGTATT
CGTTGTAGATCCCGAGGAGGGTTTCCTCTTCCGTGCAATGGACCCATCACATGTCATAATGTTGAATATGAGATTTCCAC
GAGACTCCTTCGAGGTATTCGAGGTAGACTCTAAGGTTGAGCTGGGAGTAAACTTTGAGGATGTAGCTAAGGTTCTACGT
AGAGCCTCGAAGGAGGACAAGCTTGAGATAACTTCTGATGGCAATACGGTCTCGTTCGCGTTCATAGGTAAGGGGTATAG
GAAGTTTACATTGCCGCTCCTCGATATTACTGCTGAGGAGCTTCCCGAGCCACAGCTAGAGTTCAAGGCGATAGTGAAAG
TTATGAGTGACGTGTATCGCGATGCGATAAAGGATGTTGAGCTTATAGGCGATGTTATAAGGTTCATAGCATCCCAGGAC
GAGTTTAAGGTAGTCTCTTCAAGTGATATTGGTGAGGCTGAAATAATCTATACGCGGGAGAGCGGTAGCATCATAGAGAT
GGAGGTTGAGGATACGCAGCAGGCCAGCTATACGCTCGAATACTTCAGCGATCTATCCGGTGCTGCTAGGGTTGCAGACA
CTATAACGATAAAGTTTAGCAGCGACATGCCCGCTCTCATAGAGCATGAACTCCAGCAAGGGGCAAAATTCAGCTTCCTC
ATAGCACCGAGGGTAGACTAG

Upstream 100 bases:

>100_bases
GCCCGTGTGGAGGGTGAGGTGGGGCCAGGCTCTAATCGGCCTCTCAAACAAGTACATAACGTAGCGGGTGTAGAAGAAAG
GTAGGGGAGGTAGATAGGGA

Downstream 100 bases:

>100_bases
CGCATGCCGCGAAGCCAGGATCCCGTAATGAGGACACGCAGGTTCCTAGAACACCTAACCCGCAAGTACTATGAGAGGGC
GCGTGTCAAACTACCCGGAG

Product: DNA polymerase sliding clamp B2

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog; PCNA [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKLVFFDARIWRYIVSAISKVIEEGVFVVDPEEGFLFRAMDPSHVIMLNMRFPRDSFEVFEVDSKVELGVNFEDVAKVLR
RASKEDKLEITSDGNTVSFAFIGKGYRKFTLPLLDITAEELPEPQLEFKAIVKVMSDVYRDAIKDVELIGDVIRFIASQD
EFKVVSSSDIGEAEIIYTRESGSIIEMEVEDTQQASYTLEYFSDLSGAARVADTITIKFSSDMPALIEHELQQGAKFSFL
IAPRVD

Sequences:

>Translated_246_residues
MKLVFFDARIWRYIVSAISKVIEEGVFVVDPEEGFLFRAMDPSHVIMLNMRFPRDSFEVFEVDSKVELGVNFEDVAKVLR
RASKEDKLEITSDGNTVSFAFIGKGYRKFTLPLLDITAEELPEPQLEFKAIVKVMSDVYRDAIKDVELIGDVIRFIASQD
EFKVVSSSDIGEAEIIYTRESGSIIEMEVEDTQQASYTLEYFSDLSGAARVADTITIKFSSDMPALIEHELQQGAKFSFL
IAPRVD
>Mature_246_residues
MKLVFFDARIWRYIVSAISKVIEEGVFVVDPEEGFLFRAMDPSHVIMLNMRFPRDSFEVFEVDSKVELGVNFEDVAKVLR
RASKEDKLEITSDGNTVSFAFIGKGYRKFTLPLLDITAEELPEPQLEFKAIVKVMSDVYRDAIKDVELIGDVIRFIASQD
EFKVVSSSDIGEAEIIYTRESGSIIEMEVEDTQQASYTLEYFSDLSGAARVADTITIKFSSDMPALIEHELQQGAKFSFL
IAPRVD

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication [H]

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family [H]

Homologues:

Organism=Homo sapiens, GI33239451, Length=257, Percent_Identity=22.9571984435798, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4505641, Length=257, Percent_Identity=22.9571984435798, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71995072, Length=221, Percent_Identity=20.8144796380091, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319564, Length=250, Percent_Identity=22.4, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585303, Length=242, Percent_Identity=23.1404958677686, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17136790, Length=250, Percent_Identity=21.6, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45552759, Length=250, Percent_Identity=21.6, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022648 [H]

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N [H]

EC number: NA

Molecular weight: Translated: 27853; Mature: 27853

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVFFDARIWRYIVSAISKVIEEGVFVVDPEEGFLFRAMDPSHVIMLNMRFPRDSFEVF
CEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEEEEECCCCCCEEE
EVDSKVELGVNFEDVAKVLRRASKEDKLEITSDGNTVSFAFIGKGYRKFTLPLLDITAEE
EECCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCEEEEEEHEECCHHH
LPEPQLEFKAIVKVMSDVYRDAIKDVELIGDVIRFIASQDEFKVVSSSDIGEAEIIYTRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEC
SGSIIEMEVEDTQQASYTLEYFSDLSGAARVADTITIKFSSDMPALIEHELQQGAKFSFL
CCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEE
IAPRVD
EECCCC
>Mature Secondary Structure
MKLVFFDARIWRYIVSAISKVIEEGVFVVDPEEGFLFRAMDPSHVIMLNMRFPRDSFEVF
CEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEEEEECCCCCCEEE
EVDSKVELGVNFEDVAKVLRRASKEDKLEITSDGNTVSFAFIGKGYRKFTLPLLDITAEE
EECCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCEEEEEEHEECCHHH
LPEPQLEFKAIVKVMSDVYRDAIKDVELIGDVIRFIASQDEFKVVSSSDIGEAEIIYTRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEC
SGSIIEMEVEDTQQASYTLEYFSDLSGAARVADTITIKFSSDMPALIEHELQQGAKFSFL
CCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEE
IAPRVD
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA