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The map label for this gene is priA

Identifier: 124028483

GI number: 124028483

Start: 1640315

End: 1641457

Strand: Reverse

Name: priA

Synonym: Hbut_1644

Alternate gene names: 124028483

Gene position: 1641457-1640315 (Counterclockwise)

Preceding gene: 124028484

Following gene: 124028482

Centisome position: 98.46

GC content: 51.53

Gene sequence:

>1143_bases
ATGCCGCGAAGCCAGGATCCCGTAATGAGGACACGCAGGTTCCTAGAACACCTAACCCGCAAGTACTATGAGAGGGCGCG
TGTCAAACTACCCGGAGACTTTAGCCTACGCGAGTTTGCTCTCCAGACCTGGACAGGTAAGAGCTATCTACGGCACCTCT
CATTCGCGAGTACAGAGGCTGTACATAGGCTACTAGTCGAGAAAGCGCCACGGCACTTCTACTATTCCTCAGCACGCTAC
GACCAGCCTGGCGCAGACGACATGGATGCTAAGGGGTGGCGCTCGGCTGACCTAACATTTGATATTGACGCAGATCATTT
GCCGGAATGTAGTGGCAGCATAGTAGAGGTTGACGGTGGTATTGAGGGGAAGACTAGCTTTATAGAAGAGGCATTATGCA
TGAGGGCTGCTGCTCTCAGAGCACAAATATTGTACGACATACTTGTCTACGAGTTGGGCTTTGACAAGTCCCGCATAGCT
ATAGAGTTTTCGGGGCATAGAGGGTTCCACGTAACAGTATACCTTGACGACTTCGATGATTATGCAAAGGCGGGATCAGA
TGTTAGGCGTGAAATAGTAAACTATGTTAAGGCTCTCGGTCTCCGTGCCGACGTACTTGAGCCATGGACAATGCTGCAGG
TGAGGAGGGGGAAGCCTATACCCATACCGCCAAACGTTCAGCTTGCGGGTGCGAGGGGTAGGGTTGCAAGGATTATACGA
CGCCTAGCACTCCGCGACGGCGCTATAGATATTGTAAAGGCTGTGGAGGGTCCCTCTACAACTTATAGTGAGGAACTTAG
AGAATACGAGGATAAGGCTCGCCAGCTCATAGGTGTCGAGATTGACGAGCAAGTTTCCGTTGATGTTAAGCGGCTTATAC
GTGTACCCTACAGCATAAACGGCAAGACGGGTCTCCTAGTAAAACCAGTTACTGTTGATGAGCTAGACGAGTTCGTAGTA
GATGAGACGCTTTCACCCTTTGCACGCGAGCCCCCAGTGAGGATACGTGTTGTTACGAGTCTCCCTAGCTCGGTGACCAT
ATTGGGTAATAGACTGAAGCTACGCGAGGGTGATAGCCCCCGCCTTCCTGCCCCAGTAGCTGTATACTTAATGGCTAAGG
GTGTGGCAGTCCTAGCCCAGTAG

Upstream 100 bases:

>100_bases
AACGATAAAGTTTAGCAGCGACATGCCCGCTCTCATAGAGCATGAACTCCAGCAAGGGGCAAAATTCAGCTTCCTCATAG
CACCGAGGGTAGACTAGCGC

Downstream 100 bases:

>100_bases
GCTGGCAGAGGGAGGTGAATAGTACGATGGCGGAGGAGTACATGCTACAGCGTGCTGTGAGGCTCCTAGGAGATCTAATT
GATAGACGTGATGGAAAACT

Product: DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPRSQDPVMRTRRFLEHLTRKYYERARVKLPGDFSLREFALQTWTGKSYLRHLSFASTEAVHRLLVEKAPRHFYYSSARY
DQPGADDMDAKGWRSADLTFDIDADHLPECSGSIVEVDGGIEGKTSFIEEALCMRAAALRAQILYDILVYELGFDKSRIA
IEFSGHRGFHVTVYLDDFDDYAKAGSDVRREIVNYVKALGLRADVLEPWTMLQVRRGKPIPIPPNVQLAGARGRVARIIR
RLALRDGAIDIVKAVEGPSTTYSEELREYEDKARQLIGVEIDEQVSVDVKRLIRVPYSINGKTGLLVKPVTVDELDEFVV
DETLSPFAREPPVRIRVVTSLPSSVTILGNRLKLREGDSPRLPAPVAVYLMAKGVAVLAQ

Sequences:

>Translated_380_residues
MPRSQDPVMRTRRFLEHLTRKYYERARVKLPGDFSLREFALQTWTGKSYLRHLSFASTEAVHRLLVEKAPRHFYYSSARY
DQPGADDMDAKGWRSADLTFDIDADHLPECSGSIVEVDGGIEGKTSFIEEALCMRAAALRAQILYDILVYELGFDKSRIA
IEFSGHRGFHVTVYLDDFDDYAKAGSDVRREIVNYVKALGLRADVLEPWTMLQVRRGKPIPIPPNVQLAGARGRVARIIR
RLALRDGAIDIVKAVEGPSTTYSEELREYEDKARQLIGVEIDEQVSVDVKRLIRVPYSINGKTGLLVKPVTVDELDEFVV
DETLSPFAREPPVRIRVVTSLPSSVTILGNRLKLREGDSPRLPAPVAVYLMAKGVAVLAQ
>Mature_379_residues
PRSQDPVMRTRRFLEHLTRKYYERARVKLPGDFSLREFALQTWTGKSYLRHLSFASTEAVHRLLVEKAPRHFYYSSARYD
QPGADDMDAKGWRSADLTFDIDADHLPECSGSIVEVDGGIEGKTSFIEEALCMRAAALRAQILYDILVYELGFDKSRIAI
EFSGHRGFHVTVYLDDFDDYAKAGSDVRREIVNYVKALGLRADVLEPWTMLQVRRGKPIPIPPNVQLAGARGRVARIIRR
LALRDGAIDIVKAVEGPSTTYSEELREYEDKARQLIGVEIDEQVSVDVKRLIRVPYSINGKTGLLVKPVTVDELDEFVVD
ETLSPFAREPPVRIRVVTSLPSSVTILGNRLKLREGDSPRLPAPVAVYLMAKGVAVLAQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_HYPBU (A2BN97)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013803.1
- ProteinModelPortal:   A2BN97
- SMR:   A2BN97
- STRING:   A2BN97
- GeneID:   4782235
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1644
- eggNOG:   arNOG05167
- HOGENOM:   HBG512871
- OMA:   SGGRGYH
- BioCyc:   HBUT415426:HBUT_1644-MONOMER
- HAMAP:   MF_00700
- InterPro:   IPR002755

Pfam domain/function: PF01896 DNA_primase_S

EC number: 2.7.7.-

Molecular weight: Translated: 42815; Mature: 42683

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: ACT_SITE 101-101 ACT_SITE 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSQDPVMRTRRFLEHLTRKYYERARVKLPGDFSLREFALQTWTGKSYLRHLSFASTEA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VHRLLVEKAPRHFYYSSARYDQPGADDMDAKGWRSADLTFDIDADHLPECSGSIVEVDGG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCC
IEGKTSFIEEALCMRAAALRAQILYDILVYELGFDKSRIAIEFSGHRGFHVTVYLDDFDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCHH
YAKAGSDVRREIVNYVKALGLRADVLEPWTMLQVRRGKPIPIPPNVQLAGARGRVARIIR
HHHCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHH
RLALRDGAIDIVKAVEGPSTTYSEELREYEDKARQLIGVEIDEQVSVDVKRLIRVPYSIN
HHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEC
GKTGLLVKPVTVDELDEFVVDETLSPFAREPPVRIRVVTSLPSSVTILGNRLKLREGDSP
CCCCEEEECCCHHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCCEEEECCEEEECCCCCC
RLPAPVAVYLMAKGVAVLAQ
CCCCHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
PRSQDPVMRTRRFLEHLTRKYYERARVKLPGDFSLREFALQTWTGKSYLRHLSFASTEA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VHRLLVEKAPRHFYYSSARYDQPGADDMDAKGWRSADLTFDIDADHLPECSGSIVEVDGG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCC
IEGKTSFIEEALCMRAAALRAQILYDILVYELGFDKSRIAIEFSGHRGFHVTVYLDDFDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCHH
YAKAGSDVRREIVNYVKALGLRADVLEPWTMLQVRRGKPIPIPPNVQLAGARGRVARIIR
HHHCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHH
RLALRDGAIDIVKAVEGPSTTYSEELREYEDKARQLIGVEIDEQVSVDVKRLIRVPYSIN
HHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEC
GKTGLLVKPVTVDELDEFVVDETLSPFAREPPVRIRVVTSLPSSVTILGNRLKLREGDSP
CCCCEEEECCCHHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCCEEEECCEEEECCCCCC
RLPAPVAVYLMAKGVAVLAQ
CCCCHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA