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The map label for this gene is rpoA2 [H]

Identifier: 124028421

GI number: 124028421

Start: 1586838

End: 1588112

Strand: Direct

Name: rpoA2 [H]

Synonym: Hbut_1575

Alternate gene names: 124028421

Gene position: 1586838-1588112 (Clockwise)

Preceding gene: 124028420

Following gene: 124028422

Centisome position: 95.18

GC content: 50.51

Gene sequence:

>1275_bases
ATGCCAGCTGTGCAGGGCCTCATACATGAATGGGAGAAGCTTGAGGAGCAGATAAGACCCGCACTCGAAGATGCTGCTAG
AAGGCTACCAAAGAGAATCTACGACGAGTTAGTAGAGAAGTTAAAGGCAACAACAGAGAAGCACGGACTTACACCCGAGG
AAGCCAAGAAGATAATTGTCAACGTCCTATATGAGTACGAGTATAGCAAGGTTGAACCAGGCGAGCCAGTGGGTACGGTG
GCAGCACAATCCATTGGCGAGCCAGGCACACAGATGACCCTACGTACATTCCACTACGCTGGCATACGTGAGTTCAACGT
TACGCTAGGCCTACCACGCTTCATAGAACTTGTGGATGCCAGGAGGGAGCCTTCAACACCAATAATGCACATTTACCTGG
ACGAGGAGCACCGCTATGATGAGAATAAGGCTAAGGAGGTTGCCCGCCGCATAGAGTTTACGAGGGTCATCAACGTAGCG
TCGGAAGTTGACATAGACCTTGTCAACATGAGAATGGTTATCAAACTTGACCCAGTAATGCTTGAGGATAAAGGAGTGAC
TGTAGAGCAGGTGAAGAAGGCCCTAGAAAGGCTTAAGCTCGGCAAGGTTGAGCAAGACCCATCCAACCCATTAGTACTCT
ACATACCGATAGAGATTAGTGAAGGCTTTAGCATTATAGACCTTCAGAAGAAGAGGGAGAAGATCCTCAACGCGAAGATA
AAGGGTATAAGGAAGATTAGACGCGTCATAATACAATCCATAACAGGGCCGGACGGCAGGACTGAATACTTCCTCGTGAC
TGAGGGCAGTAATCTCAAGGAGGTCCTTGAAGTCCCCGGTGTTGATCCGAGGCGCATCTACACGAATAGCATCCATGAGA
TCGAAGAGGTTCTCGGCATAGAGGCCGCTAGGACTATGCTGATAAGGGAGATGAAAGATGTACTTGATGAACAAGGTCTA
GACGTAGACATAAGGCACTTAATGCTTGTAGCCGACATAATGACCATGACTGGTAGAGTTAGACAGATAGGTAGGCATGG
TGTAAGCGGTGAGAAGCCTAGCCCATTCGCTAGGGCAGCCTTCGAGATGACCACCCAGAACCTCTACGCTGCTGCAGTTG
AGGGCGAGGTTGATAGGCTGCTAGGTGTAGCCGAGACCGTTATAGTTGGCGGGGTCATACGTGTAGGCACCGGGATGGTT
GAGGTACGTATGAGCCCGACCGCGCTAGCCAGAACAGTTAAGGGCTCCACCGGAGCCGGTGCTGGGTCGGGGTGA

Upstream 100 bases:

>100_bases
TATGGCGAAGACGGTGTAGACCCGATGAAGAGCGCCCATGGCAAGGCCGTAAACATAGACCGCGAAATAGAGCGTGTAAT
AGGCTGGAGGGTGTAGCGGG

Downstream 100 bases:

>100_bases
GCCGTTATGAGTCAGGTTCAGGTTACGGATATCGAAAAGGAGCTTGTCAACGCCCTCAAAACAGGCAAGGTCATACTCGG
CTCCCGGAAAACGCTCAAGT

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPAVQGLIHEWEKLEEQIRPALEDAARRLPKRIYDELVEKLKATTEKHGLTPEEAKKIIVNVLYEYEYSKVEPGEPVGTV
AAQSIGEPGTQMTLRTFHYAGIREFNVTLGLPRFIELVDARREPSTPIMHIYLDEEHRYDENKAKEVARRIEFTRVINVA
SEVDIDLVNMRMVIKLDPVMLEDKGVTVEQVKKALERLKLGKVEQDPSNPLVLYIPIEISEGFSIIDLQKKREKILNAKI
KGIRKIRRVIIQSITGPDGRTEYFLVTEGSNLKEVLEVPGVDPRRIYTNSIHEIEEVLGIEAARTMLIREMKDVLDEQGL
DVDIRHLMLVADIMTMTGRVRQIGRHGVSGEKPSPFARAAFEMTTQNLYAAAVEGEVDRLLGVAETVIVGGVIRVGTGMV
EVRMSPTALARTVKGSTGAGAGSG

Sequences:

>Translated_424_residues
MPAVQGLIHEWEKLEEQIRPALEDAARRLPKRIYDELVEKLKATTEKHGLTPEEAKKIIVNVLYEYEYSKVEPGEPVGTV
AAQSIGEPGTQMTLRTFHYAGIREFNVTLGLPRFIELVDARREPSTPIMHIYLDEEHRYDENKAKEVARRIEFTRVINVA
SEVDIDLVNMRMVIKLDPVMLEDKGVTVEQVKKALERLKLGKVEQDPSNPLVLYIPIEISEGFSIIDLQKKREKILNAKI
KGIRKIRRVIIQSITGPDGRTEYFLVTEGSNLKEVLEVPGVDPRRIYTNSIHEIEEVLGIEAARTMLIREMKDVLDEQGL
DVDIRHLMLVADIMTMTGRVRQIGRHGVSGEKPSPFARAAFEMTTQNLYAAAVEGEVDRLLGVAETVIVGGVIRVGTGMV
EVRMSPTALARTVKGSTGAGAGSG
>Mature_423_residues
PAVQGLIHEWEKLEEQIRPALEDAARRLPKRIYDELVEKLKATTEKHGLTPEEAKKIIVNVLYEYEYSKVEPGEPVGTVA
AQSIGEPGTQMTLRTFHYAGIREFNVTLGLPRFIELVDARREPSTPIMHIYLDEEHRYDENKAKEVARRIEFTRVINVAS
EVDIDLVNMRMVIKLDPVMLEDKGVTVEQVKKALERLKLGKVEQDPSNPLVLYIPIEISEGFSIIDLQKKREKILNAKIK
GIRKIRRVIIQSITGPDGRTEYFLVTEGSNLKEVLEVPGVDPRRIYTNSIHEIEEVLGIEAARTMLIREMKDVLDEQGLD
VDIRHLMLVADIMTMTGRVRQIGRHGVSGEKPSPFARAAFEMTTQNLYAAAVEGEVDRLLGVAETVIVGGVIRVGTGMVE
VRMSPTALARTVKGSTGAGAGSG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=407, Percent_Identity=33.1695331695332, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4505939, Length=421, Percent_Identity=31.1163895486936, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI103471997, Length=167, Percent_Identity=37.7245508982036, Blast_Score=106, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25145495, Length=363, Percent_Identity=33.0578512396694, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71987878, Length=156, Percent_Identity=39.1025641025641, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71998295, Length=122, Percent_Identity=42.6229508196721, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320061, Length=420, Percent_Identity=31.9047619047619, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324690, Length=385, Percent_Identity=33.5064935064935, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324917, Length=139, Percent_Identity=31.6546762589928, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360912, Length=362, Percent_Identity=34.2541436464088, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI17530899, Length=457, Percent_Identity=29.7592997811816, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17647875, Length=216, Percent_Identity=32.4074074074074, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 47435; Mature: 47304

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVQGLIHEWEKLEEQIRPALEDAARRLPKRIYDELVEKLKATTEKHGLTPEEAKKIIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVLYEYEYSKVEPGEPVGTVAAQSIGEPGTQMTLRTFHYAGIREFNVTLGLPRFIELVDA
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHC
RREPSTPIMHIYLDEEHRYDENKAKEVARRIEFTRVINVASEVDIDLVNMRMVIKLDPVM
CCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCEE
LEDKGVTVEQVKKALERLKLGKVEQDPSNPLVLYIPIEISEGFSIIDLQKKREKILNAKI
ECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHH
KGIRKIRRVIIQSITGPDGRTEYFLVTEGSNLKEVLEVPGVDPRRIYTNSIHEIEEVLGI
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EAARTMLIREMKDVLDEQGLDVDIRHLMLVADIMTMTGRVRQIGRHGVSGEKPSPFARAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FEMTTQNLYAAAVEGEVDRLLGVAETVIVGGVIRVGTGMVEVRMSPTALARTVKGSTGAG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEEECHHHHHHHHCCCCCCC
AGSG
CCCC
>Mature Secondary Structure 
PAVQGLIHEWEKLEEQIRPALEDAARRLPKRIYDELVEKLKATTEKHGLTPEEAKKIIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVLYEYEYSKVEPGEPVGTVAAQSIGEPGTQMTLRTFHYAGIREFNVTLGLPRFIELVDA
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHC
RREPSTPIMHIYLDEEHRYDENKAKEVARRIEFTRVINVASEVDIDLVNMRMVIKLDPVM
CCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCEE
LEDKGVTVEQVKKALERLKLGKVEQDPSNPLVLYIPIEISEGFSIIDLQKKREKILNAKI
ECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHH
KGIRKIRRVIIQSITGPDGRTEYFLVTEGSNLKEVLEVPGVDPRRIYTNSIHEIEEVLGI
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EAARTMLIREMKDVLDEQGLDVDIRHLMLVADIMTMTGRVRQIGRHGVSGEKPSPFARAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FEMTTQNLYAAAVEGEVDRLLGVAETVIVGGVIRVGTGMVEVRMSPTALARTVKGSTGAG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEEECHHHHHHHHCCCCCCC
AGSG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]