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The map label for this gene is rtcA

Identifier: 124028407

GI number: 124028407

Start: 1570944

End: 1572023

Strand: Direct

Name: rtcA

Synonym: Hbut_1561

Alternate gene names: 124028407

Gene position: 1570944-1572023 (Clockwise)

Preceding gene: 124028406

Following gene: 124028410

Centisome position: 94.23

GC content: 53.24

Gene sequence:

>1080_bases
TTGATAGTTATTGATGGCAGTATCGGTGAGGGTGGAGGCCAGATTCTACGGACAACACTGGCTCTGGCAGCACTGCTCGG
GAAACCGGTTAGGATAGTCAATATTCGCGCTAAGAGGCCAAGGCCTGGGCTACAGAGACAGCATCTTACCTCTGTTAAGG
CTGTTGCAGAGTTAGCATCTGCCAGGGTTGAGGGCTTAGAGCTTGGATCTACTACGCTTGTATTCATCCCTCGCGGTCTC
CGGAGCGGCAGGTTTTACTTCAACATTGGCACTGCTGGCAGCATTACACTTGTCCTACAAGCTCTCCTCCCGGTTACAGC
GTTTGCTCCAGGCCCGGTTGAGGTGGAAATTGTAGGTGGTACCGATGTGCCGTGGAGTCCTCCAATCGACTATGTACGGT
TTGTACTCAGAAAGCTACTAGCAATGTTCGGCTTCGAGTTTGAGATCATTGTTAAGCGTAGGGGACACTATCCTAGGGGT
GGTGGTAGAGTAGTTCTACGGGTGACACAGCCCCCTCACGTGTTGAAGCCTGTAAAGCTCGAGGAACGAGGCAAGGTGCT
GCGTGTTGAAGGCTTATCTCATGCTGTCAGGCTTCCACGCCATGTTGCTGAGAGGCAGGCTAGGAGCGCAGAAGCTGTAC
TACGCTCAAAACTTCCAGGAGTACCCATATCCATTGATCTTGAATGGTATGAGCCTAGCCGCGACCCACACCTAGGGCCT
GGAAGCGGTGTCGTAGTCTGGGCTGTTGCTGAGCACAGCGTACTTGGCAGTGACTCACTCGGCGCTAAGGGTAAGCCTGC
AGAGGCTGTTGGTAGGGAGGCTGCCGAGAAACTACTAGAGGATCTCGCCACGGGCACCGCTCTTGACCGCCATGCTTCCG
ACATGCTGATACCGTATGCAGCGCTTGCTTGTGGAGAATCAATACTTGGTGGTGCAAGGCTGACAATGCACGCCTGGACT
AACATCGAGGTTGTTAAGATGCTCGTGCCGGGAGCTGAGATGGAGTTTATTGAAGGTGGCAAGCTGAATGAGAAGTTTAA
GCTGAGGGTGAAGGGTATCTGCTACAAGCCTAGCTCCTAG

Upstream 100 bases:

>100_bases
CTTGTACCACCCTCGGTTGCAAGATTTATAGACGAGATTAAGGGTGTTGATAGGCTCAGGGCTATAGCATCACACGACTA
GCATAGGGAGGGCTTGGACC

Downstream 100 bases:

>100_bases
GCTCTGCTCCAGGGAAGAGCCAAGGCGTTTTACGCTTCAAACACTCAATAACCACATCTAGCAGCTCGCCTACACCTTGC
CCATGCAGTGCAGATATCGG

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIVIDGSIGEGGGQILRTTLALAALLGKPVRIVNIRAKRPRPGLQRQHLTSVKAVAELASARVEGLELGSTTLVFIPRGL
RSGRFYFNIGTAGSITLVLQALLPVTAFAPGPVEVEIVGGTDVPWSPPIDYVRFVLRKLLAMFGFEFEIIVKRRGHYPRG
GGRVVLRVTQPPHVLKPVKLEERGKVLRVEGLSHAVRLPRHVAERQARSAEAVLRSKLPGVPISIDLEWYEPSRDPHLGP
GSGVVVWAVAEHSVLGSDSLGAKGKPAEAVGREAAEKLLEDLATGTALDRHASDMLIPYAALACGESILGGARLTMHAWT
NIEVVKMLVPGAEMEFIEGGKLNEKFKLRVKGICYKPSS

Sequences:

>Translated_359_residues
MIVIDGSIGEGGGQILRTTLALAALLGKPVRIVNIRAKRPRPGLQRQHLTSVKAVAELASARVEGLELGSTTLVFIPRGL
RSGRFYFNIGTAGSITLVLQALLPVTAFAPGPVEVEIVGGTDVPWSPPIDYVRFVLRKLLAMFGFEFEIIVKRRGHYPRG
GGRVVLRVTQPPHVLKPVKLEERGKVLRVEGLSHAVRLPRHVAERQARSAEAVLRSKLPGVPISIDLEWYEPSRDPHLGP
GSGVVVWAVAEHSVLGSDSLGAKGKPAEAVGREAAEKLLEDLATGTALDRHASDMLIPYAALACGESILGGARLTMHAWT
NIEVVKMLVPGAEMEFIEGGKLNEKFKLRVKGICYKPSS
>Mature_359_residues
MIVIDGSIGEGGGQILRTTLALAALLGKPVRIVNIRAKRPRPGLQRQHLTSVKAVAELASARVEGLELGSTTLVFIPRGL
RSGRFYFNIGTAGSITLVLQALLPVTAFAPGPVEVEIVGGTDVPWSPPIDYVRFVLRKLLAMFGFEFEIIVKRRGHYPRG
GGRVVLRVTQPPHVLKPVKLEERGKVLRVEGLSHAVRLPRHVAERQARSAEAVLRSKLPGVPISIDLEWYEPSRDPHLGP
GSGVVVWAVAEHSVLGSDSLGAKGKPAEAVGREAAEKLLEDLATGTALDRHASDMLIPYAALACGESILGGARLTMHAWT
NIEVVKMLVPGAEMEFIEGGKLNEKFKLRVKGICYKPSS

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=336, Percent_Identity=37.202380952381, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI195927044, Length=349, Percent_Identity=35.8166189111748, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI157426877, Length=293, Percent_Identity=29.6928327645051, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI48994937, Length=331, Percent_Identity=40.4833836858006, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI32564710, Length=312, Percent_Identity=26.6025641025641, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324564, Length=310, Percent_Identity=23.5483870967742, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18543269, Length=345, Percent_Identity=37.1014492753623, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI18860065, Length=305, Percent_Identity=25.9016393442623, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_HYPBU (A2BN21)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013727.1
- ProteinModelPortal:   A2BN21
- SMR:   A2BN21
- STRING:   A2BN21
- GeneID:   4781412
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1561
- eggNOG:   arNOG04553
- HOGENOM:   HBG683539
- OMA:   SINVERR
- PhylomeDB:   A2BN21
- ProtClustDB:   PRK04204
- BioCyc:   HBUT415426:HBUT_1561-MONOMER
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 38710; Mature: 38710

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 317-317 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIDGSIGEGGGQILRTTLALAALLGKPVRIVNIRAKRPRPGLQRQHLTSVKAVAELAS
CEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ARVEGLELGSTTLVFIPRGLRSGRFYFNIGTAGSITLVLQALLPVTAFAPGPVEVEIVGG
HHHCCEECCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
TDVPWSPPIDYVRFVLRKLLAMFGFEFEIIVKRRGHYPRGGGRVVLRVTQPPHVLKPVKL
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
EERGKVLRVEGLSHAVRLPRHVAERQARSAEAVLRSKLPGVPISIDLEWYEPSRDPHLGP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC
GSGVVVWAVAEHSVLGSDSLGAKGKPAEAVGREAAEKLLEDLATGTALDRHASDMLIPYA
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHH
ALACGESILGGARLTMHAWTNIEVVKMLVPGAEMEFIEGGKLNEKFKLRVKGICYKPSS
HHHHCHHHCCCCEEEEEECCCEEEEEEECCCCCEEEECCCCCCCEEEEEEEEEEECCCC
>Mature Secondary Structure
MIVIDGSIGEGGGQILRTTLALAALLGKPVRIVNIRAKRPRPGLQRQHLTSVKAVAELAS
CEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ARVEGLELGSTTLVFIPRGLRSGRFYFNIGTAGSITLVLQALLPVTAFAPGPVEVEIVGG
HHHCCEECCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
TDVPWSPPIDYVRFVLRKLLAMFGFEFEIIVKRRGHYPRGGGRVVLRVTQPPHVLKPVKL
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
EERGKVLRVEGLSHAVRLPRHVAERQARSAEAVLRSKLPGVPISIDLEWYEPSRDPHLGP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC
GSGVVVWAVAEHSVLGSDSLGAKGKPAEAVGREAAEKLLEDLATGTALDRHASDMLIPYA
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHH
ALACGESILGGARLTMHAWTNIEVVKMLVPGAEMEFIEGGKLNEKFKLRVKGICYKPSS
HHHHCHHHCCCCEEEEEECCCEEEEEEECCCCCEEEECCCCCCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA