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The map label for this gene is rio1 [H]

Identifier: 124028253

GI number: 124028253

Start: 1404188

End: 1404967

Strand: Direct

Name: rio1 [H]

Synonym: Hbut_1404

Alternate gene names: 124028253

Gene position: 1404188-1404967 (Clockwise)

Preceding gene: 124028252

Following gene: 124028254

Centisome position: 84.23

GC content: 58.08

Gene sequence:

>780_bases
TTGTTTGAGACTGTGGAGGAGGTCTGGGATAGTCAGACCCTGCTGGCGGCCTACGAGGTTATGAGGAGGCTCCGCATAGA
CCGTTTTGCTGGCGTTGTTAGTGCGGGGAAGGAGGCCCGGGTCTACCGGGCGATCGGAAGGGACGGCAAGGAGTACGCCG
TGAAGATCTACCTTACGGTTACCGCTGAGTTCCGGAAGAGCATCCAGAAGTACCTCCTTGGCGACCCTCGGTTCGAGGGC
GTAGACATAAGCAATACCAAGAAGCTGTTCTTCGCCTGGGCCCGCAAGGAGTACCGGAACTTGAAGAGAATGTGGGAGGC
TGGCGTAAACGTCCCCCGGCCGTACCTGGTCTACCAGAACATCATAGTGATGGAGTTTCTGGGCCGCAACGGGCTCAGAG
CCCCGACACTACACGAGGTAAAGCACGAGCTGGAGCCCGAGGAGGCGGAGAAGCTGGCGTGGGAGGCGGTAGACCAGTAT
GCCCGCATCTACTGCTGCGCTAGGCTGGTACACGCCGACTATAGCGAGTACAACCTAATGCTCCTAGACGGCAAGCTATA
CGTGATAGATGTGGCCCAGGCTGTCTCGCTGGAACACCCCAACGCCGAGGACTTCCTACGTCACGACATTGAGAACATAT
ACCGGTTCTTCCATAAACAACTCGGCGTGGAGCTACCCGAGCCCCAGGAGCTACTCGAGAGGGTGAAACAATGCAGGATA
CAGTGCGTCGAGAGGAAAGTGGAGGAGGAAACTCAGCGGGCCAGCAGGAGGGCAGGGTGA

Upstream 100 bases:

>100_bases
TGTTAGAGCCTTGTAGCGGTCTACCCCGGATCTGGAAGGGTTGTAGCTGGGGTGTTGGCTGCTGGGCCGGCGTAGGGGGC
GTAGGATCAAGGATAAGGAC

Downstream 100 bases:

>100_bases
CCCCAGGCCTCCTAAGGCTCTACGCTAAGGTGCCACCGGACCGTATCGGCGTGCTGATAGGCGAAGGCGGCAAGGTGAAG
ATAGAGGTTATGAAGAGGAC

Product: putative kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MFETVEEVWDSQTLLAAYEVMRRLRIDRFAGVVSAGKEARVYRAIGRDGKEYAVKIYLTVTAEFRKSIQKYLLGDPRFEG
VDISNTKKLFFAWARKEYRNLKRMWEAGVNVPRPYLVYQNIIVMEFLGRNGLRAPTLHEVKHELEPEEAEKLAWEAVDQY
ARIYCCARLVHADYSEYNLMLLDGKLYVIDVAQAVSLEHPNAEDFLRHDIENIYRFFHKQLGVELPEPQELLERVKQCRI
QCVERKVEEETQRASRRAG

Sequences:

>Translated_259_residues
MFETVEEVWDSQTLLAAYEVMRRLRIDRFAGVVSAGKEARVYRAIGRDGKEYAVKIYLTVTAEFRKSIQKYLLGDPRFEG
VDISNTKKLFFAWARKEYRNLKRMWEAGVNVPRPYLVYQNIIVMEFLGRNGLRAPTLHEVKHELEPEEAEKLAWEAVDQY
ARIYCCARLVHADYSEYNLMLLDGKLYVIDVAQAVSLEHPNAEDFLRHDIENIYRFFHKQLGVELPEPQELLERVKQCRI
QCVERKVEEETQRASRRAG
>Mature_259_residues
MFETVEEVWDSQTLLAAYEVMRRLRIDRFAGVVSAGKEARVYRAIGRDGKEYAVKIYLTVTAEFRKSIQKYLLGDPRFEG
VDISNTKKLFFAWARKEYRNLKRMWEAGVNVPRPYLVYQNIIVMEFLGRNGLRAPTLHEVKHELEPEEAEKLAWEAVDQY
ARIYCCARLVHADYSEYNLMLLDGKLYVIDVAQAVSLEHPNAEDFLRHDIENIYRFFHKQLGVELPEPQELLERVKQCRI
QCVERKVEEETQRASRRAG

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=215, Percent_Identity=39.0697674418605, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI22325377, Length=280, Percent_Identity=32.8571428571429, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI23510358, Length=126, Percent_Identity=40.4761904761905, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI229331971, Length=228, Percent_Identity=27.1929824561404, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI229331967, Length=213, Percent_Identity=27.2300469483568, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115532073, Length=231, Percent_Identity=41.5584415584416, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17556977, Length=225, Percent_Identity=36.8888888888889, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71989043, Length=135, Percent_Identity=42.2222222222222, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324693, Length=250, Percent_Identity=38.8, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324122, Length=227, Percent_Identity=25.5506607929515, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24662811, Length=250, Percent_Identity=41.2, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21356879, Length=250, Percent_Identity=41.2, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19920700, Length=238, Percent_Identity=33.1932773109244, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 30452; Mature: 30452

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00109 PROTEIN_KINASE_TYR ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETVEEVWDSQTLLAAYEVMRRLRIDRFAGVVSAGKEARVYRAIGRDGKEYAVKIYLTV
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEEEE
TAEFRKSIQKYLLGDPRFEGVDISNTKKLFFAWARKEYRNLKRMWEAGVNVPRPYLVYQN
EHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IIVMEFLGRNGLRAPTLHEVKHELEPEEAEKLAWEAVDQYARIYCCARLVHADYSEYNLM
HHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LLDGKLYVIDVAQAVSLEHPNAEDFLRHDIENIYRFFHKQLGVELPEPQELLERVKQCRI
EECCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QCVERKVEEETQRASRRAG
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFETVEEVWDSQTLLAAYEVMRRLRIDRFAGVVSAGKEARVYRAIGRDGKEYAVKIYLTV
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEEEE
TAEFRKSIQKYLLGDPRFEGVDISNTKKLFFAWARKEYRNLKRMWEAGVNVPRPYLVYQN
EHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IIVMEFLGRNGLRAPTLHEVKHELEPEEAEKLAWEAVDQYARIYCCARLVHADYSEYNLM
HHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LLDGKLYVIDVAQAVSLEHPNAEDFLRHDIENIYRFFHKQLGVELPEPQELLERVKQCRI
EECCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QCVERKVEEETQRASRRAG
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]