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The map label for this gene is hydB [H]

Identifier: 124028218

GI number: 124028218

Start: 1358229

End: 1360154

Strand: Reverse

Name: hydB [H]

Synonym: Hbut_1368

Alternate gene names: 124028218

Gene position: 1360154-1358229 (Counterclockwise)

Preceding gene: 124028219

Following gene: 124028217

Centisome position: 81.58

GC content: 53.22

Gene sequence:

>1926_bases
ATGACACGTACATCACGCACAATTAGGATCCATATAGACCCTATAACGAGGATTGAGGGGCACCTCGGACTCCGGCTCCA
CGTAGATGCAGAGACTAGGAAGCCTACCGGCGAGGCATGGACATTCGTAACAATGTTCCGTGGCTTCGAAATCTTCAGTA
TAGGCAGACCGCCGGAAGACCTGCCGCACGTAACCAGCAGGATTTGTGGTGTATGTGGCGCCAGCCATGCTAATGCTAGC
GTTCTAGCAGTAGACATGGCATATGGTGTTAGCCCAAAGCCCATGGGTGTTGTGCTTAGAAACATGGCATTCGCTATGAC
CGACCACATCTACGACCATAGCATTATTCTCGGCATGCTCGAGGGCCCCGACTATAGTGCAGCAATAGTCTCGAAGTTTA
CGCCAAGCGTATATGACCACGCCAAGAAGGTCTACGCTGAGCACAGGGATATACATGGCTTTACAACCATAGCAGACATA
ATGGAGGCCCTTAACCCGCTCACCGGCAAGGTGTGGCAGTACGCGGTTAGGTTCCAGAGGCTTGCTAGAGAAGCTGGCGT
ACTTATCTACGGTAGGCACAGCCACCCATCAACACTGATACCTGGCGGCATATCCACTGACATAAGCGATGCAGAAAACC
TACTACTAGCCTATACGACACGCCTCATCAAGCTAACTGCGTGGGTAAAATGGTTCTACGCTCTATGGGAGGACCTGCTA
AGCTTCTTCGAGAAAGAAGTAACGCTGCCGGGAGGCCTGCCATACTCCTGCAACGGGCTAAGTTACGCCTACACCAGCGA
GCCCAGCGAGTGCCCAGGCATGAAGACAACCGTCCTAGCAGTAACAAGCAGCGGTATATTCGACGACCCCGAGGCGTATG
GCGAGGACATGCCACAGTACGACCCAGTAGAATTCTACAAGGCCATAGACAAGGCCGCTATTAAGCGCCTACTAAAGCCG
GGTATAGTGATACGCGGTGAACTCGTCTCCACAAGCCTTAAGGAGTACCAGCGCAGCATAATGGAGCACGTTGAAAGGGC
GTTCTACGAGGACTGGGATAAGCCACGCTGGTATCGAGAACAAGATCCCGAGGGGAGCCCGCTGCTGCACGGCAAGTATG
ACGCAAAGTACCATCCATGGAACAAGGTTACCATACCGAAGCTTGAGGGCAAAGCAATCAACTGGAGTGGAAAGTACACA
TGGGCAACACATGTAAGGCTAGTATGGAAGGACGGCTCTATACTACCATTCGAGGTAGGCCCCTATGCAAGGCTGCTCAT
AACATCAATGCACAACTACAGCGTTAACGTTGACGGTGCTGATGCATGGTTTAAGGCTGGTGGGAGGGAGATAACTGTAC
ACCTGCCACGCGCTTGTACAGACGAGCTACCAGGCTCTGTCTGCGAGCCAATGGAGTTCAAGTGGAAGGTGCCACCAATA
AGCACGACGATATACAGGCTATGGGCTAGAGCATTCAACCTACTGTTAGACGTAGTGGGCGCCTGGAAGGGTGTATCACA
GTATCGCAGCCTACTAGATAAGCGGTATACCGAGACATCGAGGCCGTGGAAGGTACCACGAACAATAACTTTCGGTTTCG
GCTTCACCGAGGCGCCACGCGGCACTGTGAGACACTGGGTAGTCCAAGAGAACGGTAAGGTGCTCAACGTGCAGATACAC
GCGCCAACCACGGGCAATGTCTCGCCACATGACAGGTTCGGATACAGCCCCTTCGAGCAGAGCGCGCTAAACAGCTGGGT
AACAGAAGAGCTGCCACCCGAGGAGTGGCAGGGCCTAGACTTCGTGAGATCGATAAGGAGCTTCGACCCATGCCTGGCAT
GCGCTGTCCACCTAGCATTCCGCAAGGACGGCAAAACCGTCAAGACCGTCGAGAAGATGCTAACACACGCATACTACACC
TTCTGA

Upstream 100 bases:

>100_bases
GTATGTGCGGGGTGTTGGAACCAAATGACAGTTCATGACCCGTACACGGTTTTTTGACAACACAAACCCCGGCTGAGCAC
AGTGACGAGGGGTGCACACA

Downstream 100 bases:

>100_bases
GCCCCCACGCCGTTTTTTAACACGGTATATCTCACCCCCAGGATATATTAGCCCCAGACTCACTTGCGCCACTTTACTGG
CCTCCCGGCTAGCCTCGCTA

Product: periplasmic hydrogenase large subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MTRTSRTIRIHIDPITRIEGHLGLRLHVDAETRKPTGEAWTFVTMFRGFEIFSIGRPPEDLPHVTSRICGVCGASHANAS
VLAVDMAYGVSPKPMGVVLRNMAFAMTDHIYDHSIILGMLEGPDYSAAIVSKFTPSVYDHAKKVYAEHRDIHGFTTIADI
MEALNPLTGKVWQYAVRFQRLAREAGVLIYGRHSHPSTLIPGGISTDISDAENLLLAYTTRLIKLTAWVKWFYALWEDLL
SFFEKEVTLPGGLPYSCNGLSYAYTSEPSECPGMKTTVLAVTSSGIFDDPEAYGEDMPQYDPVEFYKAIDKAAIKRLLKP
GIVIRGELVSTSLKEYQRSIMEHVERAFYEDWDKPRWYREQDPEGSPLLHGKYDAKYHPWNKVTIPKLEGKAINWSGKYT
WATHVRLVWKDGSILPFEVGPYARLLITSMHNYSVNVDGADAWFKAGGREITVHLPRACTDELPGSVCEPMEFKWKVPPI
STTIYRLWARAFNLLLDVVGAWKGVSQYRSLLDKRYTETSRPWKVPRTITFGFGFTEAPRGTVRHWVVQENGKVLNVQIH
APTTGNVSPHDRFGYSPFEQSALNSWVTEELPPEEWQGLDFVRSIRSFDPCLACAVHLAFRKDGKTVKTVEKMLTHAYYT
F

Sequences:

>Translated_641_residues
MTRTSRTIRIHIDPITRIEGHLGLRLHVDAETRKPTGEAWTFVTMFRGFEIFSIGRPPEDLPHVTSRICGVCGASHANAS
VLAVDMAYGVSPKPMGVVLRNMAFAMTDHIYDHSIILGMLEGPDYSAAIVSKFTPSVYDHAKKVYAEHRDIHGFTTIADI
MEALNPLTGKVWQYAVRFQRLAREAGVLIYGRHSHPSTLIPGGISTDISDAENLLLAYTTRLIKLTAWVKWFYALWEDLL
SFFEKEVTLPGGLPYSCNGLSYAYTSEPSECPGMKTTVLAVTSSGIFDDPEAYGEDMPQYDPVEFYKAIDKAAIKRLLKP
GIVIRGELVSTSLKEYQRSIMEHVERAFYEDWDKPRWYREQDPEGSPLLHGKYDAKYHPWNKVTIPKLEGKAINWSGKYT
WATHVRLVWKDGSILPFEVGPYARLLITSMHNYSVNVDGADAWFKAGGREITVHLPRACTDELPGSVCEPMEFKWKVPPI
STTIYRLWARAFNLLLDVVGAWKGVSQYRSLLDKRYTETSRPWKVPRTITFGFGFTEAPRGTVRHWVVQENGKVLNVQIH
APTTGNVSPHDRFGYSPFEQSALNSWVTEELPPEEWQGLDFVRSIRSFDPCLACAVHLAFRKDGKTVKTVEKMLTHAYYT
F
>Mature_640_residues
TRTSRTIRIHIDPITRIEGHLGLRLHVDAETRKPTGEAWTFVTMFRGFEIFSIGRPPEDLPHVTSRICGVCGASHANASV
LAVDMAYGVSPKPMGVVLRNMAFAMTDHIYDHSIILGMLEGPDYSAAIVSKFTPSVYDHAKKVYAEHRDIHGFTTIADIM
EALNPLTGKVWQYAVRFQRLAREAGVLIYGRHSHPSTLIPGGISTDISDAENLLLAYTTRLIKLTAWVKWFYALWEDLLS
FFEKEVTLPGGLPYSCNGLSYAYTSEPSECPGMKTTVLAVTSSGIFDDPEAYGEDMPQYDPVEFYKAIDKAAIKRLLKPG
IVIRGELVSTSLKEYQRSIMEHVERAFYEDWDKPRWYREQDPEGSPLLHGKYDAKYHPWNKVTIPKLEGKAINWSGKYTW
ATHVRLVWKDGSILPFEVGPYARLLITSMHNYSVNVDGADAWFKAGGREITVHLPRACTDELPGSVCEPMEFKWKVPPIS
TTIYRLWARAFNLLLDVVGAWKGVSQYRSLLDKRYTETSRPWKVPRTITFGFGFTEAPRGTVRHWVVQENGKVLNVQIHA
PTTGNVSPHDRFGYSPFEQSALNSWVTEELPPEEWQGLDFVRSIRSFDPCLACAVHLAFRKDGKTVKTVEKMLTHAYYTF

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=678, Percent_Identity=26.5486725663717, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787207, Length=661, Percent_Identity=27.0801815431165, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 72626; Mature: 72495

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTSRTIRIHIDPITRIEGHLGLRLHVDAETRKPTGEAWTFVTMFRGFEIFSIGRPPED
CCCCCCEEEEEECCHHEECCCEEEEEEECCCCCCCCCCCEEHHHHHCCCEEEECCCCCHH
LPHVTSRICGVCGASHANASVLAVDMAYGVSPKPMGVVLRNMAFAMTDHIYDHSIILGML
HHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
EGPDYSAAIVSKFTPSVYDHAKKVYAEHRDIHGFTTIADIMEALNPLTGKVWQYAVRFQR
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
LAREAGVLIYGRHSHPSTLIPGGISTDISDAENLLLAYTTRLIKLTAWVKWFYALWEDLL
HHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFFEKEVTLPGGLPYSCNGLSYAYTSEPSECPGMKTTVLAVTSSGIFDDPEAYGEDMPQY
HHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCC
DPVEFYKAIDKAAIKRLLKPGIVIRGELVSTSLKEYQRSIMEHVERAFYEDWDKPRWYRE
CHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QDPEGSPLLHGKYDAKYHPWNKVTIPKLEGKAINWSGKYTWATHVRLVWKDGSILPFEVG
CCCCCCCEEECCCCCCCCCCCCEECCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECC
PYARLLITSMHNYSVNVDGADAWFKAGGREITVHLPRACTDELPGSVCEPMEFKWKVPPI
CHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCEEECCCC
STTIYRLWARAFNLLLDVVGAWKGVSQYRSLLDKRYTETSRPWKVPRTITFGFGFTEAPR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
GTVRHWVVQENGKVLNVQIHAPTTGNVSPHDRFGYSPFEQSALNSWVTEELPPEEWQGLD
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCHH
FVRSIRSFDPCLACAVHLAFRKDGKTVKTVEKMLTHAYYTF
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRTSRTIRIHIDPITRIEGHLGLRLHVDAETRKPTGEAWTFVTMFRGFEIFSIGRPPED
CCCCCEEEEEECCHHEECCCEEEEEEECCCCCCCCCCCEEHHHHHCCCEEEECCCCCHH
LPHVTSRICGVCGASHANASVLAVDMAYGVSPKPMGVVLRNMAFAMTDHIYDHSIILGML
HHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
EGPDYSAAIVSKFTPSVYDHAKKVYAEHRDIHGFTTIADIMEALNPLTGKVWQYAVRFQR
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
LAREAGVLIYGRHSHPSTLIPGGISTDISDAENLLLAYTTRLIKLTAWVKWFYALWEDLL
HHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFFEKEVTLPGGLPYSCNGLSYAYTSEPSECPGMKTTVLAVTSSGIFDDPEAYGEDMPQY
HHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCC
DPVEFYKAIDKAAIKRLLKPGIVIRGELVSTSLKEYQRSIMEHVERAFYEDWDKPRWYRE
CHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QDPEGSPLLHGKYDAKYHPWNKVTIPKLEGKAINWSGKYTWATHVRLVWKDGSILPFEVG
CCCCCCCEEECCCCCCCCCCCCEECCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECC
PYARLLITSMHNYSVNVDGADAWFKAGGREITVHLPRACTDELPGSVCEPMEFKWKVPPI
CHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCEEECCCC
STTIYRLWARAFNLLLDVVGAWKGVSQYRSLLDKRYTETSRPWKVPRTITFGFGFTEAPR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
GTVRHWVVQENGKVLNVQIHAPTTGNVSPHDRFGYSPFEQSALNSWVTEELPPEEWQGLD
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCHH
FVRSIRSFDPCLACAVHLAFRKDGKTVKTVEKMLTHAYYTF
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]