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The map label for this gene is argF

Identifier: 124028062

GI number: 124028062

Start: 1214078

End: 1215022

Strand: Direct

Name: argF

Synonym: Hbut_1203

Alternate gene names: 124028062

Gene position: 1214078-1215022 (Clockwise)

Preceding gene: 124028060

Following gene: 124028064

Centisome position: 72.82

GC content: 53.33

Gene sequence:

>945_bases
TTGCCCGGCCCCCTCTACGGCCGCGACCTTTTATCAATACGTGACCTCAGCAGCGAGGAGGTATGGCTAGTCATTGAAAC
TGCTAGGCAGATGAAGCTACGCTACTACGCTGGTGAGCGCATCATACCCATGCTAAAGGGTAAGACTATCGCCCTCATCT
TCGAGAAGCCTAGTACGCGTACAAGGGTCAGCATGGAGGTTGCCGCGCTACAACTCGGCGCGACCCCCCTGACATTCAGA
CGCGACGAGCTACAGCTGGCCCGCGGCGAGCCAATTAAGGATACAGCACGCGTACTATCGCGCTACGTGGACGCAATAGC
CGCAAGGGTCTTCAAGCATGAGAGCCTGGAAGAAATGGCTGCCTATGCAAGCGTACCCGTTATAAACATGCTCAGCGACC
TCGAACATCCCTTGCAAGCTCTAGCGGATGCCTTAACGATTTACGAGAAGAAGGGCCACATAAAGGGCATCAAAGTCGTA
TACGTGGGTGATGGTAGAAACAATGTGGCGCACAGCCTCCTACTGGTCATCGCTAAGCTCGGGGGACACATAGTTATATC
ATCGCCTAAGGAGCTGACACCGAGAAAAGACATCCTCGAAGCCGCACAGCTGGCAGCAAAGGAGACAGGAGCAACAATAG
AGCTTATTGAGGATCCCGCTGAGGCTGTTAGGGGTGCCGACGTGGTCTATACCGATGTCTGGGTGAGCATGGGTGAGGAA
TCCCTAGCCGAGGAAAGGAGGCGTCTATTACAGCGATACCAGGTTAACGAGAAACTAATGTCACTAGCATCAAACAATGC
CATATTCATGCACTGCCTACCGGCCCATCGCGGTGAGGAGGTAACCGAGGAAGTCATAGAAGGCCCATGGAGTGTCGTGT
GGGATCAAGCTGAGAATAGGCTACACGCACAAAAGGCTGTTCTAGCACTGATATTAGCGCCTTAA

Upstream 100 bases:

>100_bases
GAGGGACTGGTATGAGGAAACTCCCATGAATGGAGCCCACATAGACTGAAGACATGTTAAAGTACCGGACCCTTACTTAT
GTTTAAACGGCTGAAGCCCC

Downstream 100 bases:

>100_bases
AACGCCGTATACTTTTCTCTTCACCTAACCCTCCTAATCTCCACGCCCAGCTCTCTAGCAGCAGTCTTCGCGTCGTCCAG
GAGTACTGGGCCTGAACCGT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPGPLYGRDLLSIRDLSSEEVWLVIETARQMKLRYYAGERIIPMLKGKTIALIFEKPSTRTRVSMEVAALQLGATPLTFR
RDELQLARGEPIKDTARVLSRYVDAIAARVFKHESLEEMAAYASVPVINMLSDLEHPLQALADALTIYEKKGHIKGIKVV
YVGDGRNNVAHSLLLVIAKLGGHIVISSPKELTPRKDILEAAQLAAKETGATIELIEDPAEAVRGADVVYTDVWVSMGEE
SLAEERRRLLQRYQVNEKLMSLASNNAIFMHCLPAHRGEEVTEEVIEGPWSVVWDQAENRLHAQKAVLALILAP

Sequences:

>Translated_314_residues
MPGPLYGRDLLSIRDLSSEEVWLVIETARQMKLRYYAGERIIPMLKGKTIALIFEKPSTRTRVSMEVAALQLGATPLTFR
RDELQLARGEPIKDTARVLSRYVDAIAARVFKHESLEEMAAYASVPVINMLSDLEHPLQALADALTIYEKKGHIKGIKVV
YVGDGRNNVAHSLLLVIAKLGGHIVISSPKELTPRKDILEAAQLAAKETGATIELIEDPAEAVRGADVVYTDVWVSMGEE
SLAEERRRLLQRYQVNEKLMSLASNNAIFMHCLPAHRGEEVTEEVIEGPWSVVWDQAENRLHAQKAVLALILAP
>Mature_313_residues
PGPLYGRDLLSIRDLSSEEVWLVIETARQMKLRYYAGERIIPMLKGKTIALIFEKPSTRTRVSMEVAALQLGATPLTFRR
DELQLARGEPIKDTARVLSRYVDAIAARVFKHESLEEMAAYASVPVINMLSDLEHPLQALADALTIYEKKGHIKGIKVVY
VGDGRNNVAHSLLLVIAKLGGHIVISSPKELTPRKDILEAAQLAAKETGATIELIEDPAEAVRGADVVYTDVWVSMGEES
LAEERRRLLQRYQVNEKLMSLASNNAIFMHCLPAHRGEEVTEEVIEGPWSVVWDQAENRLHAQKAVLALILAP

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=40.453074433657, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.6527331189711, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1790703, Length=335, Percent_Identity=41.7910447761194, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1786469, Length=335, Percent_Identity=41.7910447761194, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI2367364, Length=323, Percent_Identity=31.8885448916409, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994908, Length=339, Percent_Identity=25.0737463126844, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=25.9036144578313, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=324, Percent_Identity=38.5802469135802, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=31.0344827586207, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=24.4372990353698, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_HYPBU (A2BM26)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013382.1
- ProteinModelPortal:   A2BM26
- SMR:   A2BM26
- STRING:   A2BM26
- GeneID:   4781565
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1203
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   IGAKLGW
- PhylomeDB:   A2BM26
- ProtClustDB:   PRK00779
- BioCyc:   HBUT415426:HBUT_1203-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34947; Mature: 34816

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 109-109 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGPLYGRDLLSIRDLSSEEVWLVIETARQMKLRYYAGERIIPMLKGKTIALIFEKPSTR
CCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCC
TRVSMEVAALQLGATPLTFRRDELQLARGEPIKDTARVLSRYVDAIAARVFKHESLEEMA
CEEHHHHHHHHCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYASVPVINMLSDLEHPLQALADALTIYEKKGHIKGIKVVYVGDGRNNVAHSLLLVIAKL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
GGHIVISSPKELTPRKDILEAAQLAAKETGATIELIEDPAEAVRGADVVYTDVWVSMGEE
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCEEEEHHHHHCCHH
SLAEERRRLLQRYQVNEKLMSLASNNAIFMHCLPAHRGEEVTEEVIEGPWSVVWDQAENR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
LHAQKAVLALILAP
HHHHHHHHHHEECC
>Mature Secondary Structure 
PGPLYGRDLLSIRDLSSEEVWLVIETARQMKLRYYAGERIIPMLKGKTIALIFEKPSTR
CCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCC
TRVSMEVAALQLGATPLTFRRDELQLARGEPIKDTARVLSRYVDAIAARVFKHESLEEMA
CEEHHHHHHHHCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYASVPVINMLSDLEHPLQALADALTIYEKKGHIKGIKVVYVGDGRNNVAHSLLLVIAKL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
GGHIVISSPKELTPRKDILEAAQLAAKETGATIELIEDPAEAVRGADVVYTDVWVSMGEE
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCEEEEHHHHHCCHH
SLAEERRRLLQRYQVNEKLMSLASNNAIFMHCLPAHRGEEVTEEVIEGPWSVVWDQAENR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
LHAQKAVLALILAP
HHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA