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The map label for this gene is 124028064

Identifier: 124028064

GI number: 124028064

Start: 1215829

End: 1217961

Strand: Direct

Name: 124028064

Synonym: Hbut_1205

Alternate gene names: NA

Gene position: 1215829-1217961 (Clockwise)

Preceding gene: 124028062

Following gene: 124028065

Centisome position: 72.93

GC content: 49.74

Gene sequence:

>2133_bases
ATGCCATTTGAGAACTACAAGCACGTAGAGAAATTTGCCAGGAATTACGGCATCTCACCCGACGTAGCAAAATTCTCCTA
CATAGACGCCAACGACCCCTGGATAGAGTACTGGCTAGCCAGGTACCTACACGATAAAGGTGTGTCGTCTTGGGCTAGCC
TTACCCGTGACAACCCTGTAACAAAGCTATTCTGGTACCCGTGGGGTAGAGACTTTACGCATACAGAGTTCCCATTCATA
CCCGTTGTTGGCGCGCTAACCCCTGGAGGCATGAGGGTTGTTGAATGGGTTTCACTGCTACCGCCAGTTTTCGGAACACT
AGCAATAATTATTGCAGCCATCTACATGTACCGTGTCTATGGACGTCCAGCAGCAATCATAGCTGCTGCTCTTCTATCAC
TCCTGCCAGCATCAACCTCAAGGACCTACGCTGGGTTCGTGGAGAAGATTGGTATTGCACTTCCCTTCCTCATTCTAGGC
CTCATGTTCTACGCCGAAGCACTTAGGAGGCGTTCGCTGGTATACGCTGTTGCTGCTGGTGCTGCATTTGGCGTTATAAG
TTATGTATGGGGAGGCTACGTTATTGCCGGCCTTGCTATCGCCGTAACTTCACTGCTAGTACCACTAGCTGTCAATAGGC
TTGATGAGGCTAAGCCTTATCTCCTGACAAGCATTACTGTAGCTGCTACCGCGCTTGCAGCATCCATACCAGCCGACATC
TACGGCCCGGCATCAATCAGGTTTTATGCCGCCGCACTCGCTGTAGCACTGTATGTGTATGGCATTGTGTTCGTTCTAGA
AAAGCTTCATGCCAGGAGTACTGTAGCGAGAGGCTTCGTCTATAGGCTCGCAGCTCGTTGGCGCAAGTTCTACACATATG
TGCTCGCAGCATCCATTATCATCGGCATGTTTATAGCGTCGCTGATCAAGCTGCCGAGCCGCGCCGTGTTTGCTATAGCC
TGGCCTCTACGGGAGACAGGGCTCATACACTTGTCCCTGCTCGCTGAGACTGTTGCCGAGCATTCATCACCACTTACAGA
TCCGAGGCTTATGAGGGACTTCATGTGGAGTACCAATGTACTAGTTGTGCTTGCACCAATAGCTGGGTTATACCTCCTCT
ATCGAGGCCTATGGAGAAGAGAGGCTTACCACATACCGCTAGCTATCACAGCTCTAGGACTGTACTATGCCGTGCTCGGA
ATGGTTTACTTCCAGCAAGCAGCATCGGTAGTAGGCATACTCGCTACAGCTGCACTAACACCCTATGTGCTAGAGCCGAT
CACTATTACGAGCATACAGCGCACAAGGAGATGGAGACGTAAGAGCCAGGGGCCCTCGGAGCTGCAGCTCCTTGGAGCCG
TAGTATTCATAGCACTCATAGCTGGTGGCATGGTGGTAAGCGCGTATAGAACAGTAAACGTCCTCGAGAAGCATGTAGCA
ATGATTACAGGGTATAGCCTAGACACTACCCAGCTGGGCTGGCTCTACCTGCTAGATTATCTCCGGCAGAGTATAAGCAA
CGATACAGTTGTCGTCACATGGTGGGACTATGGGTACCAGATAAGTGTTGGTGCCGAACGCCCCTCAGTAGCTGATGGTG
CAACGATTAATGCTACCCAGATACGACTACTAGCAGAGTTCTACACAGCCACAAGCGAAGACGAAGCCGCCTCGATACTC
GAAAGGCTCGGGCTAAAGCCTAACCAGACCTTGATATTCGTACACGATCTGGCACTCTACAACCCATCAAAAGGCATACT
TGTGTATACACCCGCTATAGACATACCAAAGTCAGCTGCCATGCTCCATATAGCTGAGAAGGACAAGGAGGGGTTCTGGC
CGATAAACGATAAGTACATGTATACGATGATATACCGGTTCTTCTCTACTGCACCATTCTACCTCGACAAGATAGGCATA
GCAGTGCCCAACGTAACTGAGCTAGTAAAGCCAAAAACCGTTCTCGTCGGCAACCTCCAGATGAAACCATTTACTATGAA
GAGGTTTGAACCATACATGGTAGTCGTAGGGTTCTACATCGACGCTGAGACAGGCCAACCAATCGCAAAAATAGTAAACG
GAGATGTGTACTATTACGTAATGCTGCTAATACTGTTTAAGTGGGTTAAATAG

Upstream 100 bases:

>100_bases
ATGAACACATCTAAACTATTAACGGTTACAACAAGTATTCAGCCATTATCATCATAGCTCTCGTGACACTGTTACAGTTA
TAGGCTTCTATGTCAGGTAC

Downstream 100 bases:

>100_bases
CTGCATCACTGACAAACTGGGCTGAGGAGGCTGAAGGCTTCAATACATAGCTCTGAGTCCCATTTATTAAGGGGGGCTAG
AGAGTAGAGGGTGCAGCGCT

Product: hypothetical protein

Products: dolichyl diphosphate; glycoprotein with the oligosaccharide chain attached by N-glycosyl ; $linkage to protein L-asparagine

Alternate protein names: Oligosaccharyl Transferase Subunit; Oligosaccharyl Transferase STT3 Like-Protein; Dolichyl-Diphosphooligosaccharide-Protein Glycotransferase

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MPFENYKHVEKFARNYGISPDVAKFSYIDANDPWIEYWLARYLHDKGVSSWASLTRDNPVTKLFWYPWGRDFTHTEFPFI
PVVGALTPGGMRVVEWVSLLPPVFGTLAIIIAAIYMYRVYGRPAAIIAAALLSLLPASTSRTYAGFVEKIGIALPFLILG
LMFYAEALRRRSLVYAVAAGAAFGVISYVWGGYVIAGLAIAVTSLLVPLAVNRLDEAKPYLLTSITVAATALAASIPADI
YGPASIRFYAAALAVALYVYGIVFVLEKLHARSTVARGFVYRLAARWRKFYTYVLAASIIIGMFIASLIKLPSRAVFAIA
WPLRETGLIHLSLLAETVAEHSSPLTDPRLMRDFMWSTNVLVVLAPIAGLYLLYRGLWRREAYHIPLAITALGLYYAVLG
MVYFQQAASVVGILATAALTPYVLEPITITSIQRTRRWRRKSQGPSELQLLGAVVFIALIAGGMVVSAYRTVNVLEKHVA
MITGYSLDTTQLGWLYLLDYLRQSISNDTVVVTWWDYGYQISVGAERPSVADGATINATQIRLLAEFYTATSEDEAASIL
ERLGLKPNQTLIFVHDLALYNPSKGILVYTPAIDIPKSAAMLHIAEKDKEGFWPINDKYMYTMIYRFFSTAPFYLDKIGI
AVPNVTELVKPKTVLVGNLQMKPFTMKRFEPYMVVVGFYIDAETGQPIAKIVNGDVYYYVMLLILFKWVK

Sequences:

>Translated_710_residues
MPFENYKHVEKFARNYGISPDVAKFSYIDANDPWIEYWLARYLHDKGVSSWASLTRDNPVTKLFWYPWGRDFTHTEFPFI
PVVGALTPGGMRVVEWVSLLPPVFGTLAIIIAAIYMYRVYGRPAAIIAAALLSLLPASTSRTYAGFVEKIGIALPFLILG
LMFYAEALRRRSLVYAVAAGAAFGVISYVWGGYVIAGLAIAVTSLLVPLAVNRLDEAKPYLLTSITVAATALAASIPADI
YGPASIRFYAAALAVALYVYGIVFVLEKLHARSTVARGFVYRLAARWRKFYTYVLAASIIIGMFIASLIKLPSRAVFAIA
WPLRETGLIHLSLLAETVAEHSSPLTDPRLMRDFMWSTNVLVVLAPIAGLYLLYRGLWRREAYHIPLAITALGLYYAVLG
MVYFQQAASVVGILATAALTPYVLEPITITSIQRTRRWRRKSQGPSELQLLGAVVFIALIAGGMVVSAYRTVNVLEKHVA
MITGYSLDTTQLGWLYLLDYLRQSISNDTVVVTWWDYGYQISVGAERPSVADGATINATQIRLLAEFYTATSEDEAASIL
ERLGLKPNQTLIFVHDLALYNPSKGILVYTPAIDIPKSAAMLHIAEKDKEGFWPINDKYMYTMIYRFFSTAPFYLDKIGI
AVPNVTELVKPKTVLVGNLQMKPFTMKRFEPYMVVVGFYIDAETGQPIAKIVNGDVYYYVMLLILFKWVK
>Mature_709_residues
PFENYKHVEKFARNYGISPDVAKFSYIDANDPWIEYWLARYLHDKGVSSWASLTRDNPVTKLFWYPWGRDFTHTEFPFIP
VVGALTPGGMRVVEWVSLLPPVFGTLAIIIAAIYMYRVYGRPAAIIAAALLSLLPASTSRTYAGFVEKIGIALPFLILGL
MFYAEALRRRSLVYAVAAGAAFGVISYVWGGYVIAGLAIAVTSLLVPLAVNRLDEAKPYLLTSITVAATALAASIPADIY
GPASIRFYAAALAVALYVYGIVFVLEKLHARSTVARGFVYRLAARWRKFYTYVLAASIIIGMFIASLIKLPSRAVFAIAW
PLRETGLIHLSLLAETVAEHSSPLTDPRLMRDFMWSTNVLVVLAPIAGLYLLYRGLWRREAYHIPLAITALGLYYAVLGM
VYFQQAASVVGILATAALTPYVLEPITITSIQRTRRWRRKSQGPSELQLLGAVVFIALIAGGMVVSAYRTVNVLEKHVAM
ITGYSLDTTQLGWLYLLDYLRQSISNDTVVVTWWDYGYQISVGAERPSVADGATINATQIRLLAEFYTATSEDEAASILE
RLGLKPNQTLIFVHDLALYNPSKGILVYTPAIDIPKSAAMLHIAEKDKEGFWPINDKYMYTMIYRFFSTAPFYLDKIGIA
VPNVTELVKPKTVLVGNLQMKPFTMKRFEPYMVVVGFYIDAETGQPIAKIVNGDVYYYVMLLILFKWVK

Specific function: Unknown

COG id: COG1287

COG function: function code R; Uncharacterized membrane protein, required for N-linked glycosylation

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI30578410, Length=637, Percent_Identity=22.9199372056515, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI22749415, Length=570, Percent_Identity=21.9298245614035, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555006, Length=587, Percent_Identity=22.3168654173765, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321416, Length=630, Percent_Identity=24.1269841269841, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129103, Length=651, Percent_Identity=22.4270353302611, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17738187, Length=587, Percent_Identity=21.8057921635434, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.4.1.119

Molecular weight: Translated: 79155; Mature: 79024

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFENYKHVEKFARNYGISPDVAKFSYIDANDPWIEYWLARYLHDKGVSSWASLTRDNPV
CCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCC
TKLFWYPWGRDFTHTEFPFIPVVGALTPGGMRVVEWVSLLPPVFGTLAIIIAAIYMYRVY
CEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRPAAIIAAALLSLLPASTSRTYAGFVEKIGIALPFLILGLMFYAEALRRRSLVYAVAAG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFGVISYVWGGYVIAGLAIAVTSLLVPLAVNRLDEAKPYLLTSITVAATALAASIPADI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
YGPASIRFYAAALAVALYVYGIVFVLEKLHARSTVARGFVYRLAARWRKFYTYVLAASII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGMFIASLIKLPSRAVFAIAWPLRETGLIHLSLLAETVAEHSSPLTDPRLMRDFMWSTNV
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
LVVLAPIAGLYLLYRGLWRREAYHIPLAITALGLYYAVLGMVYFQQAASVVGILATAALT
HHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYVLEPITITSIQRTRRWRRKSQGPSELQLLGAVVFIALIAGGMVVSAYRTVNVLEKHVA
HHHCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MITGYSLDTTQLGWLYLLDYLRQSISNDTVVVTWWDYGYQISVGAERPSVADGATINATQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCEECHHH
IRLLAEFYTATSEDEAASILERLGLKPNQTLIFVHDLALYNPSKGILVYTPAIDIPKSAA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCHH
MLHIAEKDKEGFWPINDKYMYTMIYRFFSTAPFYLDKIGIAVPNVTELVKPKTVLVGNLQ
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCEEEEECCE
MKPFTMKRFEPYMVVVGFYIDAETGQPIAKIVNGDVYYYVMLLILFKWVK
ECCCHHHHCCCEEEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFENYKHVEKFARNYGISPDVAKFSYIDANDPWIEYWLARYLHDKGVSSWASLTRDNPV
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCC
TKLFWYPWGRDFTHTEFPFIPVVGALTPGGMRVVEWVSLLPPVFGTLAIIIAAIYMYRVY
CEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRPAAIIAAALLSLLPASTSRTYAGFVEKIGIALPFLILGLMFYAEALRRRSLVYAVAAG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFGVISYVWGGYVIAGLAIAVTSLLVPLAVNRLDEAKPYLLTSITVAATALAASIPADI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
YGPASIRFYAAALAVALYVYGIVFVLEKLHARSTVARGFVYRLAARWRKFYTYVLAASII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGMFIASLIKLPSRAVFAIAWPLRETGLIHLSLLAETVAEHSSPLTDPRLMRDFMWSTNV
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
LVVLAPIAGLYLLYRGLWRREAYHIPLAITALGLYYAVLGMVYFQQAASVVGILATAALT
HHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYVLEPITITSIQRTRRWRRKSQGPSELQLLGAVVFIALIAGGMVVSAYRTVNVLEKHVA
HHHCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MITGYSLDTTQLGWLYLLDYLRQSISNDTVVVTWWDYGYQISVGAERPSVADGATINATQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCEECHHH
IRLLAEFYTATSEDEAASILERLGLKPNQTLIFVHDLALYNPSKGILVYTPAIDIPKSAA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCHH
MLHIAEKDKEGFWPINDKYMYTMIYRFFSTAPFYLDKIGIAVPNVTELVKPKTVLVGNLQ
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCEEEEECCE
MKPFTMKRFEPYMVVVGFYIDAETGQPIAKIVNGDVYYYVMLLILFKWVK
ECCCHHHHCCCEEEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dolichyl diphosphooligosaccharide; protein L-asparagine

Specific reaction: dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide $chain attached by N-glycosyl linkage to protein L-asparagine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA