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The map label for this gene is birA [C]

Identifier: 124028020

GI number: 124028020

Start: 1169823

End: 1170533

Strand: Direct

Name: birA [C]

Synonym: Hbut_1158

Alternate gene names: 124028020

Gene position: 1169823-1170533 (Clockwise)

Preceding gene: 124028017

Following gene: 124028021

Centisome position: 70.17

GC content: 66.95

Gene sequence:

>711_bases
GTGCTTGGGCAGCTTAGCGTTTTAGAGGTATACGACGCTATAGATACGACTATGGACGCCACGCCCCCCAGGCTCCCGGC
CGCTGTCGCCGCGCTCACACAGCGCCGGGGCCGTGGCAGAACTGGGGCCTGGCACAGCCCTCGGGGCGGCGCATGGATAA
CGGTATTCCTCAACGCACCGCCCGACCCCAGGCTCCCCACAGCTGTTGGGGGCTACCTCGCAGGGCTCCTCGAGGAGTTC
CTATGCGGCGCCGTACACCTCACCGTCAAATGGCCAAACGACGTCTACGCGGGCGGGAGGAAGCTCGCCGGCATCCTGGT
CGAGCACCGTGCGGGAATGCTACGGGTGGGCGTGGGGGTGAACGTCTACAACGAGCCGCCGCCGGGCGCGACGTCGCTAG
CCCAGCTGGGCTACGGGGGTCCGCTAGCGCTGGTCTACGCCGCCATCATAGAGGCTGTTATGAGGGCTGCGGAGGACCCC
GCAGGCGGCGTCGCGGAGGCTGCCGCCAGGGACATGCTCTGGGGCTGCAGGGTAGCCATAGAATCCCCCGAGGGGCTCGT
CGAGGGAGTAGCCAGAGGCATCACTAGCAGTGGAGCCCTACTCGTCGAGACGGGTGAGGGGACCGTGGAGCTGGCGTGCT
GCCACGTAAAAGCCTGGAGCTGCAGTAACACCAGGCCAGCCACCAAGTCAAGCCCGGCGGGAGGAGACTAG

Upstream 100 bases:

>100_bases
ACGTGTGGATAGCGGATAAATAAGTAAGGGTTTTTGACAGGTGGCGAGATAGGTGTACGGCGGGTGTCGAACACTGTGCT
ACTCAGCGCAGCTCCGCCAG

Downstream 100 bases:

>100_bases
CCCCGGGATGGCCCGGCCTTGATACTCTCAGACCGCGACATAGCATGGTACATCGAGAAGGGCCTCCTCCGGATAGACCC
ACTACTAGACGACACGATAA

Product: biotin/lipoate A/B protein ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Alternate protein names: Biotin-Acetyl-CoA-Carboxylase Ligase; Biotin-Protein Ligase; Biotin-(Acetyl-CoA-Carboxylase) Ligase; Biotin-(Acetyl-CoA-Carboxylase) Synthetase; Biotin Operon Repressor; Bira Biotin-(Acetyl-CoA-Carboxylase) Ligase; Biotin-(Acetyl-CoA Carboxylase) Ligase; Biotin Acetyl-CoA-Carboxylase Synthetase; Bifunctional Protein BirA; Biotin-(Acetyl-CoA Carboxylase) Synthetase; Biotin/Lipoate A/B Protein Ligase; Biotin Ligase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLGQLSVLEVYDAIDTTMDATPPRLPAAVAALTQRRGRGRTGAWHSPRGGAWITVFLNAPPDPRLPTAVGGYLAGLLEEF
LCGAVHLTVKWPNDVYAGGRKLAGILVEHRAGMLRVGVGVNVYNEPPPGATSLAQLGYGGPLALVYAAIIEAVMRAAEDP
AGGVAEAAARDMLWGCRVAIESPEGLVEGVARGITSSGALLVETGEGTVELACCHVKAWSCSNTRPATKSSPAGGD

Sequences:

>Translated_236_residues
MLGQLSVLEVYDAIDTTMDATPPRLPAAVAALTQRRGRGRTGAWHSPRGGAWITVFLNAPPDPRLPTAVGGYLAGLLEEF
LCGAVHLTVKWPNDVYAGGRKLAGILVEHRAGMLRVGVGVNVYNEPPPGATSLAQLGYGGPLALVYAAIIEAVMRAAEDP
AGGVAEAAARDMLWGCRVAIESPEGLVEGVARGITSSGALLVETGEGTVELACCHVKAWSCSNTRPATKSSPAGGD
>Mature_236_residues
MLGQLSVLEVYDAIDTTMDATPPRLPAAVAALTQRRGRGRTGAWHSPRGGAWITVFLNAPPDPRLPTAVGGYLAGLLEEF
LCGAVHLTVKWPNDVYAGGRKLAGILVEHRAGMLRVGVGVNVYNEPPPGATSLAQLGYGGPLALVYAAIIEAVMRAAEDP
AGGVAEAAARDMLWGCRVAIESPEGLVEGVARGITSSGALLVETGEGTVELACCHVKAWSCSNTRPATKSSPAGGD

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15

Molecular weight: Translated: 24364; Mature: 24364

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGQLSVLEVYDAIDTTMDATPPRLPAAVAALTQRRGRGRTGAWHSPRGGAWITVFLNAP
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
PDPRLPTAVGGYLAGLLEEFLCGAVHLTVKWPNDVYAGGRKLAGILVEHRAGMLRVGVGV
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCE
NVYNEPPPGATSLAQLGYGGPLALVYAAIIEAVMRAAEDPAGGVAEAAARDMLWGCRVAI
EECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEE
ESPEGLVEGVARGITSSGALLVETGEGTVELACCHVKAWSCSNTRPATKSSPAGGD
CCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGQLSVLEVYDAIDTTMDATPPRLPAAVAALTQRRGRGRTGAWHSPRGGAWITVFLNAP
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
PDPRLPTAVGGYLAGLLEEFLCGAVHLTVKWPNDVYAGGRKLAGILVEHRAGMLRVGVGV
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCE
NVYNEPPPGATSLAQLGYGGPLALVYAAIIEAVMRAAEDPAGGVAEAAARDMLWGCRVAI
EECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEE
ESPEGLVEGVARGITSSGALLVETGEGTVELACCHVKAWSCSNTRPATKSSPAGGD
CCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA