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The map label for this gene is ampH [C]

Identifier: 124027904

GI number: 124027904

Start: 1048446

End: 1049750

Strand: Direct

Name: ampH [C]

Synonym: Hbut_1035

Alternate gene names: 124027904

Gene position: 1048446-1049750 (Clockwise)

Preceding gene: 124027903

Following gene: 124027905

Centisome position: 62.89

GC content: 57.85

Gene sequence:

>1305_bases
TTGCCTTTACCTCCTTCCCGCGACGTTCTAGGATATGTTGTTAGGCGAATGTCCAAAACTATGACGCCTGGTCTCAGCCT
CGCCGTGAATGCCAACAATGATATCTATGCGCGTGGCTTCGGCTACCGTAGCCTTGAGAAGGGGTTGCCTGCCACGCCCG
ACACGATATACTGTATCGCTTCCATAACAAAGCCCATTACAGCTGTTGCCGTCATGCAGCTCGTCGAGGCCGGAAAGCTC
AGCCTTGACGACCCGGCCGATAAGTATCTCCCTGTAGAGTTGCGTGTGCGCGGTGAGCCGGTTCGGATATGGCACCTGCT
CAGCCATACTAGCGGCATACCCGCGCTTGGCTATGCCGAGGCCCTTCTGACCGGGTACCTGGGGCTTGACAGACGCTGGC
TTCCATTCTCTGAGCCCATGGCTGTGCTTGAATGGCTTGAGCGTGGAGCACGGCGCTGGGCTGTTGCGACTCCTGGTGAG
AGGTTCTTCTACCTCAACGAGGGCTATGTAGCGCTTGGCGTTATCATCGAAAGAGTTTCCGGCGTCAAATTCGAGGAGTA
TGTGGAGAGGAACATCTTCAAGCCACTGGGCATGTCCTCGTCAACCCTTGACGCCTCTAGGGCCCAGTCCTCCCCTCTAT
ATGCAACCCCCTACGATGCATCCACGACACCGCCACAGCCAGCAATGATTCCCACTGGGATAACAGCTGATGGTGGAGCC
TATAGCACCGTGCTAGACCTCGTAAAGTTCATGTCAATGCTCGCTAACAGGGGGAGGCTAGGTGACACCGAGATATTGTC
GCCTAGCAGTGTGGAGGAGATGGAGAAACCGAGGGTCCAGCTACCGAGCCAGATATGGGGCAACGACAGCTACGGCCTAG
GCCTCATAATATACGATGGGTTCCCCGGTGGACGCCTCGTAGGCCATAGTGGTAGCGTCTACGTGCACACGGGCTTTGCC
GGCTACATCCGCGGCAAGAGTGTTATTGTGAGCGTGCTGGCCAACGCTGACCCGGGCGCGACAACTATAGGCATGGCATT
GGCCGCTGACGCTGCCGGGATTGACTACCATGTGCTGCCAATGGTGAGGGCTGAGGAGGCTGCTGAGAAGCTCGAGGGTA
TCTACCATGGCTACGAGGGCACGGTGAGGTTCCGGGTCAAAGCCCTGGGTGACGCCCTGGTCATAGAGAGTCTGAGCCGG
CCGGGACTCCAGGAGGTCATGGTGCCAGAGCGCATAGAGCCGCCGGTCTACGAGTACACCGTATACCGTGGCGGCCGGAG
AATGAAGGCAGTGTTCACCCGTTGA

Upstream 100 bases:

>100_bases
CTAGCTCTTCGTGAGGATGTACGGTATGTGGCCGGGGTATAGTCCACTTTCTTAACCCTGGAGCTACCCGTGTAGTGTCT
CCGGGGCGGGTGGCTGGGTC

Downstream 100 bases:

>100_bases
CCCGGAAACAGGCAAGGTTGAGCTGATCTACGAGAGGTACCGCCTCGTAAAAGTGGCCGAGGCCAGTGCCGCCGGCGGTG
TCTAACATGTCCCGTTACGC

Product: Beta-lactamase class C

Products: substituted beta-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPLPPSRDVLGYVVRRMSKTMTPGLSLAVNANNDIYARGFGYRSLEKGLPATPDTIYCIASITKPITAVAVMQLVEAGKL
SLDDPADKYLPVELRVRGEPVRIWHLLSHTSGIPALGYAEALLTGYLGLDRRWLPFSEPMAVLEWLERGARRWAVATPGE
RFFYLNEGYVALGVIIERVSGVKFEEYVERNIFKPLGMSSSTLDASRAQSSPLYATPYDASTTPPQPAMIPTGITADGGA
YSTVLDLVKFMSMLANRGRLGDTEILSPSSVEEMEKPRVQLPSQIWGNDSYGLGLIIYDGFPGGRLVGHSGSVYVHTGFA
GYIRGKSVIVSVLANADPGATTIGMALAADAAGIDYHVLPMVRAEEAAEKLEGIYHGYEGTVRFRVKALGDALVIESLSR
PGLQEVMVPERIEPPVYEYTVYRGGRRMKAVFTR

Sequences:

>Translated_434_residues
MPLPPSRDVLGYVVRRMSKTMTPGLSLAVNANNDIYARGFGYRSLEKGLPATPDTIYCIASITKPITAVAVMQLVEAGKL
SLDDPADKYLPVELRVRGEPVRIWHLLSHTSGIPALGYAEALLTGYLGLDRRWLPFSEPMAVLEWLERGARRWAVATPGE
RFFYLNEGYVALGVIIERVSGVKFEEYVERNIFKPLGMSSSTLDASRAQSSPLYATPYDASTTPPQPAMIPTGITADGGA
YSTVLDLVKFMSMLANRGRLGDTEILSPSSVEEMEKPRVQLPSQIWGNDSYGLGLIIYDGFPGGRLVGHSGSVYVHTGFA
GYIRGKSVIVSVLANADPGATTIGMALAADAAGIDYHVLPMVRAEEAAEKLEGIYHGYEGTVRFRVKALGDALVIESLSR
PGLQEVMVPERIEPPVYEYTVYRGGRRMKAVFTR
>Mature_433_residues
PLPPSRDVLGYVVRRMSKTMTPGLSLAVNANNDIYARGFGYRSLEKGLPATPDTIYCIASITKPITAVAVMQLVEAGKLS
LDDPADKYLPVELRVRGEPVRIWHLLSHTSGIPALGYAEALLTGYLGLDRRWLPFSEPMAVLEWLERGARRWAVATPGER
FFYLNEGYVALGVIIERVSGVKFEEYVERNIFKPLGMSSSTLDASRAQSSPLYATPYDASTTPPQPAMIPTGITADGGAY
STVLDLVKFMSMLANRGRLGDTEILSPSSVEEMEKPRVQLPSQIWGNDSYGLGLIIYDGFPGGRLVGHSGSVYVHTGFAG
YIRGKSVIVSVLANADPGATTIGMALAADAAGIDYHVLPMVRAEEAAEKLEGIYHGYEGTVRFRVKALGDALVIESLSRP
GLQEVMVPERIEPPVYEYTVYRGGRRMKAVFTR

Specific function: Binds Penicillin And Contributes To The Normal Morphology Of Escherichia Coli. [C]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI26051231, Length=113, Percent_Identity=38.9380530973451, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI26051233, Length=113, Percent_Identity=38.9380530973451, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786574, Length=235, Percent_Identity=26.8085106382979, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082101, Length=288, Percent_Identity=25.6944444444444, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790593, Length=195, Percent_Identity=23.0769230769231, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17550934, Length=304, Percent_Identity=27.6315789473684, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533026, Length=330, Percent_Identity=28.1818181818182, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565630, Length=332, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6

Molecular weight: Translated: 47342; Mature: 47211

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPPSRDVLGYVVRRMSKTMTPGLSLAVNANNDIYARGFGYRSLEKGLPATPDTIYCIA
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHCCCCCCCCEEEEE
SITKPITAVAVMQLVEAGKLSLDDPADKYLPVELRVRGEPVRIWHLLSHTSGIPALGYAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEHHCCCCCCHHHHH
ALLTGYLGLDRRWLPFSEPMAVLEWLERGARRWAVATPGERFFYLNEGYVALGVIIERVS
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHC
GVKFEEYVERNIFKPLGMSSSTLDASRAQSSPLYATPYDASTTPPQPAMIPTGITADGGA
CCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCEECCCCCCCCCH
YSTVLDLVKFMSMLANRGRLGDTEILSPSSVEEMEKPRVQLPSQIWGNDSYGLGLIIYDG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEEECC
FPGGRLVGHSGSVYVHTGFAGYIRGKSVIVSVLANADPGATTIGMALAADAAGIDYHVLP
CCCCEEECCCCCEEEEECEEHHHCCHHHEEHEECCCCCCCHHHHHHHHHCCCCCCEEEEC
MVRAEEAAEKLEGIYHGYEGTVRFRVKALGDALVIESLSRPGLQEVMVPERIEPPVYEYT
HHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHHCCCCHHHHCCCCCCCCCCEEEE
VYRGGRRMKAVFTR
EEECCCEEEEEECC
>Mature Secondary Structure 
PLPPSRDVLGYVVRRMSKTMTPGLSLAVNANNDIYARGFGYRSLEKGLPATPDTIYCIA
CCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHCCCCCCCCEEEEE
SITKPITAVAVMQLVEAGKLSLDDPADKYLPVELRVRGEPVRIWHLLSHTSGIPALGYAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEHHCCCCCCHHHHH
ALLTGYLGLDRRWLPFSEPMAVLEWLERGARRWAVATPGERFFYLNEGYVALGVIIERVS
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHC
GVKFEEYVERNIFKPLGMSSSTLDASRAQSSPLYATPYDASTTPPQPAMIPTGITADGGA
CCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCEECCCCCCCCCH
YSTVLDLVKFMSMLANRGRLGDTEILSPSSVEEMEKPRVQLPSQIWGNDSYGLGLIIYDG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEEECC
FPGGRLVGHSGSVYVHTGFAGYIRGKSVIVSVLANADPGATTIGMALAADAAGIDYHVLP
CCCCEEECCCCCEEEEECEEHHHCCHHHEEHEECCCCCCCHHHHHHHHHCCCCCCEEEEC
MVRAEEAAEKLEGIYHGYEGTVRFRVKALGDALVIESLSRPGLQEVMVPERIEPPVYEYT
HHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHHCCCCHHHHCCCCCCCCCCEEEE
VYRGGRRMKAVFTR
EEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]