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The map label for this gene is pdxT

Identifier: 124027902

GI number: 124027902

Start: 1046069

End: 1046677

Strand: Direct

Name: pdxT

Synonym: Hbut_1033

Alternate gene names: 124027902

Gene position: 1046069-1046677 (Clockwise)

Preceding gene: 124027901

Following gene: 124027903

Centisome position: 62.75

GC content: 50.9

Gene sequence:

>609_bases
ATGGTTGTAGTAGGTGTTCTCGCGCTGCAGGGGGACTGGATTGAGCATATAGAGCTTCTAAGAGAAATTGAAGGCGTTGA
AGCCGTACCTGTTAAGACGGAGAAGCAGCTTGAAAGCATAGATGCGCTGATTATACCTGGCGGAGAGTCAACAACCATAG
GAAGGCTCATAGAGCGTAGGGGACTCCTAGAGCCTCTACGGGACAGGATTAGGGCAGGCCTACCCGTACTTGGAACTTGT
GCTGGAAGCATACTGCTAGCTAAAAAGGTTCGTGATAGGGTTGTAGGCCCCGTCGAGCAGCCCCTACTAGCTGTTATGGA
TATAGCTGTTCTACGTAACGCGTTTGGCAGACAGATGAACTCCTTCGAGGCAGAAGTGCACATTGAAGGTGTAGGTACTG
TTCACGCAGCCTTCATAAGAGCACCAATAATTGAGGAGACATGGGGAGAGGCTAGACCCATAGCAAAGCTGAACCATCCC
CTGCTGGGGGAAGTTACAGTAGCCGCTAGGCAAAAGGCAATAATCGCTACTGTATTCCACCCAGAGATAACAGGCGACAG
TAAGCTCCACAAGCTTCTAGTAGCTGCAGCCAAGGGGAGGGCCTTCTAG

Upstream 100 bases:

>100_bases
CGGAAGCCCAGTCGATGGTTTCAGAGAGGAACGCGATGCCGGGTATAGACATTACTAGGCTCAAGCCCGAAGAGCTGTTG
CAGATTAGAGGTGAGTAGCC

Downstream 100 bases:

>100_bases
ACCTTGACATTTAGGTTGGAGGGTATCGCAAGCAAGTGTCTACGGTGCGGCTACGAGCTTCACAATGTTGTTTTGCCCCG
CTGCCCACGCTGCGGATACA

Product: glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MVVVGVLALQGDWIEHIELLREIEGVEAVPVKTEKQLESIDALIIPGGESTTIGRLIERRGLLEPLRDRIRAGLPVLGTC
AGSILLAKKVRDRVVGPVEQPLLAVMDIAVLRNAFGRQMNSFEAEVHIEGVGTVHAAFIRAPIIEETWGEARPIAKLNHP
LLGEVTVAARQKAIIATVFHPEITGDSKLHKLLVAAAKGRAF

Sequences:

>Translated_202_residues
MVVVGVLALQGDWIEHIELLREIEGVEAVPVKTEKQLESIDALIIPGGESTTIGRLIERRGLLEPLRDRIRAGLPVLGTC
AGSILLAKKVRDRVVGPVEQPLLAVMDIAVLRNAFGRQMNSFEAEVHIEGVGTVHAAFIRAPIIEETWGEARPIAKLNHP
LLGEVTVAARQKAIIATVFHPEITGDSKLHKLLVAAAKGRAF
>Mature_202_residues
MVVVGVLALQGDWIEHIELLREIEGVEAVPVKTEKQLESIDALIIPGGESTTIGRLIERRGLLEPLRDRIRAGLPVLGTC
AGSILLAKKVRDRVVGPVEQPLLAVMDIAVLRNAFGRQMNSFEAEVHIEGVGTVHAAFIRAPIIEETWGEARPIAKLNHP
LLGEVTVAARQKAIIATVFHPEITGDSKLHKLLVAAAKGRAF

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=212, Percent_Identity=37.2641509433962, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323995, Length=216, Percent_Identity=36.1111111111111, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=215, Percent_Identity=35.8139534883721, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_HYPBU (A2BLL6)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013222.1
- ProteinModelPortal:   A2BLL6
- SMR:   A2BLL6
- STRING:   A2BLL6
- GeneID:   4782000
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1033
- eggNOG:   arNOG08412
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   A2BLL6
- BioCyc:   HBUT415426:HBUT_1033-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21882; Mature: 21882

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 80-80 ACT_SITE 180-180 ACT_SITE 182-182 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVVGVLALQGDWIEHIELLREIEGVEAVPVKTEKQLESIDALIIPGGESTTIGRLIERR
CEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHC
GLLEPLRDRIRAGLPVLGTCAGSILLAKKVRDRVVGPVEQPLLAVMDIAVLRNAFGRQMN
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
SFEAEVHIEGVGTVHAAFIRAPIIEETWGEARPIAKLNHPLLGEVTVAARQKAIIATVFH
CCEEEEEEECCCHHHHHHHHCCCHHHHCCCCCCHHHCCCCCCCHHHHHHHHHEEEEEEEC
PEITGDSKLHKLLVAAAKGRAF
CCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVVVGVLALQGDWIEHIELLREIEGVEAVPVKTEKQLESIDALIIPGGESTTIGRLIERR
CEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHC
GLLEPLRDRIRAGLPVLGTCAGSILLAKKVRDRVVGPVEQPLLAVMDIAVLRNAFGRQMN
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
SFEAEVHIEGVGTVHAAFIRAPIIEETWGEARPIAKLNHPLLGEVTVAARQKAIIATVFH
CCEEEEEEECCCHHHHHHHHCCCHHHHCCCCCCHHHCCCCCCCHHHHHHHHHEEEEEEEC
PEITGDSKLHKLLVAAAKGRAF
CCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA