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The map label for this gene is pheT [H]

Identifier: 124027762

GI number: 124027762

Start: 889184

End: 890854

Strand: Direct

Name: pheT [H]

Synonym: Hbut_0888

Alternate gene names: 124027762

Gene position: 889184-890854 (Clockwise)

Preceding gene: 124027761

Following gene: 124027763

Centisome position: 53.34

GC content: 52.12

Gene sequence:

>1671_bases
ATGCCAGTTCTCAGATTTAAGCCCGGTAGGGTGGAGGAGGTCATAGGAGTACAGCTCGACCAGGCATTGAAGCTAATGGA
GAGGTTAAAGATCGAGGTTGAAATAGACGAGGAAGGTTACGTGGTAGCAGAGCTAGAGGTAGACCGGCCCGACATGTATA
GCCTAGAGGGTATCGGTCGCCAGGTCAAAGGCTTGTTGGGGGTAGAGAGGGGGCTTCCCCGCTACAACATCGTCGAGACA
GACTATAGGATCGTAGCAGATGATGTACCCACGAGACCCTATGTCCTTGGTGCAATAGTATGGGACGTTGATGTTGACGA
GGACTTCTTAGAGGAGCTAATACAGTTCCAGGAAAAGCTACACGTAAGCCATGGAGATAAGAGGCGTAGGGTCGCAATAG
GTCTCCACGATCTCGACAAGCTTCCGGGCAAGACTATCCACTACAAGTTCGAGCATGTGGACATGGTGAGGTTTACACCG
CTGCACGGCGATAGGGAGATGAGCCTTAGGGAGGTGTTTAACGAGACACCTCAAGGCAGAGAGTATAGTGGCATAGCTCT
TCAGGGCGATAGGCATCCCGTACTCTACGCTAACGGCGAGGTTATCAGTGTGCCACCAGTGATAAATGCGGAGCTTACAA
AGGTTGAGCCTGGAACCCGTCACCTCTTCATTGATGTTACGGGGCCCGAGCTGGGCATTGTAGAGGATATCCTAGCCATA
CTAGCTACAAACCTTGCTGAACGTAGCCGTACAAAGAGGATAGGTCTTGTAAGGGTTGAAGCGCCATGGGGTAATCGTGT
AGTACCAGGTCTTGAGCCTCACCGGTGGCGGCTCAGACTTGCAACTGTTGAGAGAGTCATAGGGTTGAAGCTCTCGGCGA
ACGAAGTCGCGCAAAAGCTTGAGGCTATGAGGTTTGGTGTGGTCGAGGTTGGAGGAGATTATGTGGACGTCCTGGTGCCC
AGGTTTAGGATAGACATTATGCACGAGGTTGACCTGGCTGAAGAGGTAGCACTAGCAATAGGCTTTGATGCCCTCAAACC
TGAGAAGCCCAAACTGATGCTGCGTGGCGGTCTGCTACCCGTACGGGCATGGGAACGTGAAGCTAGGAAGCTCCTAGCAG
GCCACGGCTACGTGGAGGTGAAAACCTATACTCTAGTCTCCTGCAGGGAGCAAGAAGCAGTAGGAGGTATACCGGCAGAG
AAGCTTGTCAAGATAGCTAATCCCGTAAGCGTTGAGGCTGACTGTGTGCGGGCAACCATGCTGCCGAATCTCCTGCGTAT
TGCAGCTTCGAACCAGCATAGAATACCACTACGCATCTTCGAGTTGGGCGAGGTTGTCGAGGTAACAGGGGAAGGTGACC
GTGGCGTGCGCTACGGCAGAAAGCTAGCAATACTATACATGGCGGACAAGGCTGGCTACGAAGACATTCAAGCAGTGGTA
TATAGTATTGTGAGATTAACAAACGACGAAATAAGCGTGATAAAAGAGTATACACATCCACTCTTGATCCAGGGCAGGAC
AGCACTAGCCAAGACAAAGCGTGGACTAAGAATAATCATTGGGGAAGTTCGCCCAGAAATCCTAGAGCGTTATGGCATCG
CGTACCCGATAGCTGTGGCTGAAATAGATTACACGGCGCTCGCAGGGGGTGCCGCTGTCCCCCGCCCCTAA

Upstream 100 bases:

>100_bases
GAGAGGCTTGCAATGGCACTCTACGACCTCACAGATATTAGGCAGCTGTACTCGCACGATGTCGAATTTCTCGAGAATAT
ACCTGTAAGGTGGTGGATCT

Downstream 100 bases:

>100_bases
CACCCAGCCTCACAGACCCCGCGTGGGGGCCTCCGGCTGGGCTCTTATAGGCGGGAGTAACGGGGCGGCGCGGCACCCCC
AGACAGTTAGCTGCATGTTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPVLRFKPGRVEEVIGVQLDQALKLMERLKIEVEIDEEGYVVAELEVDRPDMYSLEGIGRQVKGLLGVERGLPRYNIVET
DYRIVADDVPTRPYVLGAIVWDVDVDEDFLEELIQFQEKLHVSHGDKRRRVAIGLHDLDKLPGKTIHYKFEHVDMVRFTP
LHGDREMSLREVFNETPQGREYSGIALQGDRHPVLYANGEVISVPPVINAELTKVEPGTRHLFIDVTGPELGIVEDILAI
LATNLAERSRTKRIGLVRVEAPWGNRVVPGLEPHRWRLRLATVERVIGLKLSANEVAQKLEAMRFGVVEVGGDYVDVLVP
RFRIDIMHEVDLAEEVALAIGFDALKPEKPKLMLRGGLLPVRAWEREARKLLAGHGYVEVKTYTLVSCREQEAVGGIPAE
KLVKIANPVSVEADCVRATMLPNLLRIAASNQHRIPLRIFELGEVVEVTGEGDRGVRYGRKLAILYMADKAGYEDIQAVV
YSIVRLTNDEISVIKEYTHPLLIQGRTALAKTKRGLRIIIGEVRPEILERYGIAYPIAVAEIDYTALAGGAAVPRP

Sequences:

>Translated_556_residues
MPVLRFKPGRVEEVIGVQLDQALKLMERLKIEVEIDEEGYVVAELEVDRPDMYSLEGIGRQVKGLLGVERGLPRYNIVET
DYRIVADDVPTRPYVLGAIVWDVDVDEDFLEELIQFQEKLHVSHGDKRRRVAIGLHDLDKLPGKTIHYKFEHVDMVRFTP
LHGDREMSLREVFNETPQGREYSGIALQGDRHPVLYANGEVISVPPVINAELTKVEPGTRHLFIDVTGPELGIVEDILAI
LATNLAERSRTKRIGLVRVEAPWGNRVVPGLEPHRWRLRLATVERVIGLKLSANEVAQKLEAMRFGVVEVGGDYVDVLVP
RFRIDIMHEVDLAEEVALAIGFDALKPEKPKLMLRGGLLPVRAWEREARKLLAGHGYVEVKTYTLVSCREQEAVGGIPAE
KLVKIANPVSVEADCVRATMLPNLLRIAASNQHRIPLRIFELGEVVEVTGEGDRGVRYGRKLAILYMADKAGYEDIQAVV
YSIVRLTNDEISVIKEYTHPLLIQGRTALAKTKRGLRIIIGEVRPEILERYGIAYPIAVAEIDYTALAGGAAVPRP
>Mature_555_residues
PVLRFKPGRVEEVIGVQLDQALKLMERLKIEVEIDEEGYVVAELEVDRPDMYSLEGIGRQVKGLLGVERGLPRYNIVETD
YRIVADDVPTRPYVLGAIVWDVDVDEDFLEELIQFQEKLHVSHGDKRRRVAIGLHDLDKLPGKTIHYKFEHVDMVRFTPL
HGDREMSLREVFNETPQGREYSGIALQGDRHPVLYANGEVISVPPVINAELTKVEPGTRHLFIDVTGPELGIVEDILAIL
ATNLAERSRTKRIGLVRVEAPWGNRVVPGLEPHRWRLRLATVERVIGLKLSANEVAQKLEAMRFGVVEVGGDYVDVLVPR
FRIDIMHEVDLAEEVALAIGFDALKPEKPKLMLRGGLLPVRAWEREARKLLAGHGYVEVKTYTLVSCREQEAVGGIPAEK
LVKIANPVSVEADCVRATMLPNLLRIAASNQHRIPLRIFELGEVVEVTGEGDRGVRYGRKLAILYMADKAGYEDIQAVVY
SIVRLTNDEISVIKEYTHPLLIQGRTALAKTKRGLRIIIGEVRPEILERYGIAYPIAVAEIDYTALAGGAAVPRP

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=527, Percent_Identity=27.5142314990512, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1788006, Length=359, Percent_Identity=25.9052924791086, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17533403, Length=536, Percent_Identity=27.2388059701493, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323089, Length=473, Percent_Identity=27.4841437632135, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21355211, Length=528, Percent_Identity=27.8409090909091, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918 [H]

Pfam domain/function: PF03484 B5 [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 62310; Mature: 62179

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLRFKPGRVEEVIGVQLDQALKLMERLKIEVEIDEEGYVVAELEVDRPDMYSLEGIGR
CCCEECCCCCHHHHHHCCHHHHHHHHHHCEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
QVKGLLGVERGLPRYNIVETDYRIVADDVPTRPYVLGAIVWDVDVDEDFLEELIQFQEKL
HHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
HVSHGDKRRRVAIGLHDLDKLPGKTIHYKFEHVDMVRFTPLHGDREMSLREVFNETPQGR
CCCCCCCCCEEEEEHHHHHHCCCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCC
EYSGIALQGDRHPVLYANGEVISVPPVINAELTKVEPGTRHLFIDVTGPELGIVEDILAI
CCCCEEEECCCCEEEEECCCEEECCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHH
LATNLAERSRTKRIGLVRVEAPWGNRVVPGLEPHRWRLRLATVERVIGLKLSANEVAQKL
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCEECHHHHHHHH
EAMRFGVVEVGGDYVDVLVPRFRIDIMHEVDLAEEVALAIGFDALKPEKPKLMLRGGLLP
HHHHCCEEEECCCEEEEECCHHEEEEHHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCCC
VRAWEREARKLLAGHGYVEVKTYTLVSCREQEAVGGIPAEKLVKIANPVSVEADCVRATM
HHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHCCCCCHHHHHHHCCCCCCCHHHHHHHH
LPNLLRIAASNQHRIPLRIFELGEVVEVTGEGDRGVRYGRKLAILYMADKAGYEDIQAVV
HHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
YSIVRLTNDEISVIKEYTHPLLIQGRTALAKTKRGLRIIIGEVRPEILERYGIAYPIAVA
HHHHHHCCCHHHHHHHCCCCEEEECCHHHHHCCCCEEEEEECCCHHHHHHCCCEEEEEEE
EIDYTALAGGAAVPRP
EECHHEECCCCCCCCC
>Mature Secondary Structure 
PVLRFKPGRVEEVIGVQLDQALKLMERLKIEVEIDEEGYVVAELEVDRPDMYSLEGIGR
CCEECCCCCHHHHHHCCHHHHHHHHHHCEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
QVKGLLGVERGLPRYNIVETDYRIVADDVPTRPYVLGAIVWDVDVDEDFLEELIQFQEKL
HHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
HVSHGDKRRRVAIGLHDLDKLPGKTIHYKFEHVDMVRFTPLHGDREMSLREVFNETPQGR
CCCCCCCCCEEEEEHHHHHHCCCCEEEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCC
EYSGIALQGDRHPVLYANGEVISVPPVINAELTKVEPGTRHLFIDVTGPELGIVEDILAI
CCCCEEEECCCCEEEEECCCEEECCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHH
LATNLAERSRTKRIGLVRVEAPWGNRVVPGLEPHRWRLRLATVERVIGLKLSANEVAQKL
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCEECHHHHHHHH
EAMRFGVVEVGGDYVDVLVPRFRIDIMHEVDLAEEVALAIGFDALKPEKPKLMLRGGLLP
HHHHCCEEEECCCEEEEECCHHEEEEHHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCCC
VRAWEREARKLLAGHGYVEVKTYTLVSCREQEAVGGIPAEKLVKIANPVSVEADCVRATM
HHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHCCCCCHHHHHHHCCCCCCCHHHHHHHH
LPNLLRIAASNQHRIPLRIFELGEVVEVTGEGDRGVRYGRKLAILYMADKAGYEDIQAVV
HHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
YSIVRLTNDEISVIKEYTHPLLIQGRTALAKTKRGLRIIIGEVRPEILERYGIAYPIAVA
HHHHHHCCCHHHHHHHCCCCEEEECCHHHHHCCCCEEEEEECCCHHHHHHCCCEEEEEEE
EIDYTALAGGAAVPRP
EECHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]