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The map label for this gene is pheS [H]

Identifier: 124027761

GI number: 124027761

Start: 887707

End: 889203

Strand: Direct

Name: pheS [H]

Synonym: Hbut_0887

Alternate gene names: 124027761

Gene position: 887707-889203 (Clockwise)

Preceding gene: 124027760

Following gene: 124027762

Centisome position: 53.25

GC content: 55.38

Gene sequence:

>1497_bases
TTGCAAGGCAAGATACCGGTTTCAGAATCTGAACATAAGCTCCTAGAGCTACTAGTCGGCCGCGTCGAGGCTGGTAGAGA
ATATGACACTGAGACCTTGGCGGAGCTGGCTGGGCTTGATAGGAGCAAGGTAGAGTCACTGGTTAGGCTCCTAGCAGAGA
AGGGGCTTGTTAGGCTCCGCGAGGAAACTATGGAGAAGTACGTTGCGACCGAGGAGGCTAAGCGTTATCTACGCGAAGGG
TTTCCCGAGGAGAAGCTTGTAAAGATCCTAGCTGGGGCCGGCGGCGAGGCGAGGCTGGATGAACTCCGTAACGCTATGGG
CCAGGAACTCTCCATAGCGCTCGCTAATGCTACGAGGAAGGGTTGGGTTGAGGTTCGCGGTGGCAGGGTTAGGCTTGTAG
TGGACCCCTCTAAGGCTGTTGCCGAGGAGAGGAAGCTCCTAGAAAAGCTTGAGATTGGAGAGCGGCTTCAGCCCAGTGAG
TTTCGCCTACTGCGACGGAGAAGGTTAGTGGAACGCCTAGCTGAAAGGAGAACTATCGTTGTGTTCCCAGAGGCTCCAGA
GAAGCTGCTAGAGCAGGTCGTCGTTGAGGTTGGTGCACTTACACGTGACCTCATAGCTTCGGGGCGTTGGAGAACGGTTC
GCCTAAGGAGATACAATGTAGCGGCTGAGCCCCCCAGGCGGCGGCTCGGCAGGCTAAACTTCTTCGTAGAGTTCATAGAG
TATCTGCGCGACGTAATGAAGGAGATGGGATTCCAAGAGGTTGATGATGCTCCAGTGGAGCTAGAGTTCTGGAACTATGA
CGTGTTGTACCAGCCGCAGTATCATCCTGCGAGAAGCCCTACCGACACCTTCTACTTGAGCAACCCCTCCCGGGGCGAGC
TGCCGAGCGAGCTTACTCCAAGGGTGGCTGAAGCGCATGAAAAGGGGCTTGCGGGCAGCAGGGGCTGGAGGTACCGGTGG
AGTCAGGAGCTTGCTGCGAGGCTGATACTGCGTAGCCACACCACAGCCGTGTCAGCGCGTGTGCTAGCCTCCAAGCCTAG
GCCGCCATTCCGCTTCTTCACCATAGGCCGCGTCTACCGTGTAGAGACGATTGACCCAAGGCATTTGCCTGAGTTCCACC
AGCTGGATGGTATAGCGAGCGAGGACGGTGTTAGCCTACGCTGGCTACTTGGCCTCCTAGCAGAATTCCTGGAGCGTATC
GGGATAAGGGAATACAAGTTTAGGCCAGCCTACTTTCCCTTTACCGAGCCCTCAGTCGAGGCCTACGTGAGGGTTCGCGA
TCAGTGGCTTGAGGTTCTAGGTGCAGGACTGTTTAGGCCGGAAATGTTGGCAGCTCTAGGCATAGACTATCCTGTAGCAG
CATGGGGCATGGGTATTGAGAGGCTTGCAATGGCACTCTACGACCTCACAGATATTAGGCAGCTGTACTCGCACGATGTC
GAATTTCTCGAGAATATACCTGTAAGGTGGTGGATCTATGCCAGTTCTCAGATTTAA

Upstream 100 bases:

>100_bases
CTGGCGAGCTATACCAGTTCTAGCCTATACAGTCACAACAATACCCCATAAGTCTATTTTTACGTCTAACCAACCTCCTC
CCGCCAAGGGCTTCAGTATC

Downstream 100 bases:

>100_bases
GCCCGGTAGGGTGGAGGAGGTCATAGGAGTACAGCTCGACCAGGCATTGAAGCTAATGGAGAGGTTAAAGATCGAGGTTG
AAATAGACGAGGAAGGTTAC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQGKIPVSESEHKLLELLVGRVEAGREYDTETLAELAGLDRSKVESLVRLLAEKGLVRLREETMEKYVATEEAKRYLREG
FPEEKLVKILAGAGGEARLDELRNAMGQELSIALANATRKGWVEVRGGRVRLVVDPSKAVAEERKLLEKLEIGERLQPSE
FRLLRRRRLVERLAERRTIVVFPEAPEKLLEQVVVEVGALTRDLIASGRWRTVRLRRYNVAAEPPRRRLGRLNFFVEFIE
YLRDVMKEMGFQEVDDAPVELEFWNYDVLYQPQYHPARSPTDTFYLSNPSRGELPSELTPRVAEAHEKGLAGSRGWRYRW
SQELAARLILRSHTTAVSARVLASKPRPPFRFFTIGRVYRVETIDPRHLPEFHQLDGIASEDGVSLRWLLGLLAEFLERI
GIREYKFRPAYFPFTEPSVEAYVRVRDQWLEVLGAGLFRPEMLAALGIDYPVAAWGMGIERLAMALYDLTDIRQLYSHDV
EFLENIPVRWWIYASSQI

Sequences:

>Translated_498_residues
MQGKIPVSESEHKLLELLVGRVEAGREYDTETLAELAGLDRSKVESLVRLLAEKGLVRLREETMEKYVATEEAKRYLREG
FPEEKLVKILAGAGGEARLDELRNAMGQELSIALANATRKGWVEVRGGRVRLVVDPSKAVAEERKLLEKLEIGERLQPSE
FRLLRRRRLVERLAERRTIVVFPEAPEKLLEQVVVEVGALTRDLIASGRWRTVRLRRYNVAAEPPRRRLGRLNFFVEFIE
YLRDVMKEMGFQEVDDAPVELEFWNYDVLYQPQYHPARSPTDTFYLSNPSRGELPSELTPRVAEAHEKGLAGSRGWRYRW
SQELAARLILRSHTTAVSARVLASKPRPPFRFFTIGRVYRVETIDPRHLPEFHQLDGIASEDGVSLRWLLGLLAEFLERI
GIREYKFRPAYFPFTEPSVEAYVRVRDQWLEVLGAGLFRPEMLAALGIDYPVAAWGMGIERLAMALYDLTDIRQLYSHDV
EFLENIPVRWWIYASSQI
>Mature_498_residues
MQGKIPVSESEHKLLELLVGRVEAGREYDTETLAELAGLDRSKVESLVRLLAEKGLVRLREETMEKYVATEEAKRYLREG
FPEEKLVKILAGAGGEARLDELRNAMGQELSIALANATRKGWVEVRGGRVRLVVDPSKAVAEERKLLEKLEIGERLQPSE
FRLLRRRRLVERLAERRTIVVFPEAPEKLLEQVVVEVGALTRDLIASGRWRTVRLRRYNVAAEPPRRRLGRLNFFVEFIE
YLRDVMKEMGFQEVDDAPVELEFWNYDVLYQPQYHPARSPTDTFYLSNPSRGELPSELTPRVAEAHEKGLAGSRGWRYRW
SQELAARLILRSHTTAVSARVLASKPRPPFRFFTIGRVYRVETIDPRHLPEFHQLDGIASEDGVSLRWLLGLLAEFLERI
GIREYKFRPAYFPFTEPSVEAYVRVRDQWLEVLGAGLFRPEMLAALGIDYPVAAWGMGIERLAMALYDLTDIRQLYSHDV
EFLENIPVRWWIYASSQI

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=490, Percent_Identity=33.469387755102, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI5729820, Length=270, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788007, Length=284, Percent_Identity=33.4507042253521, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32563657, Length=491, Percent_Identity=34.0122199592668, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17508957, Length=491, Percent_Identity=34.0122199592668, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI32566635, Length=268, Percent_Identity=27.9850746268657, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321087, Length=443, Percent_Identity=32.5056433408578, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18858079, Length=494, Percent_Identity=32.9959514170041, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137424, Length=208, Percent_Identity=32.2115384615385, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 57500; Mature: 57500

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKIPVSESEHKLLELLVGRVEAGREYDTETLAELAGLDRSKVESLVRLLAEKGLVRLR
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EETMEKYVATEEAKRYLREGFPEEKLVKILAGAGGEARLDELRNAMGQELSIALANATRK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEEECCCCCC
GWVEVRGGRVRLVVDPSKAVAEERKLLEKLEIGERLQPSEFRLLRRRRLVERLAERRTIV
CCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VFPEAPEKLLEQVVVEVGALTRDLIASGRWRTVRLRRYNVAAEPPRRRLGRLNFFVEFIE
EECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
YLRDVMKEMGFQEVDDAPVELEFWNYDVLYQPQYHPARSPTDTFYLSNPSRGELPSELTP
HHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCH
RVAEAHEKGLAGSRGWRYRWSQELAARLILRSHTTAVSARVLASKPRPPFRFFTIGRVYR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEE
VETIDPRHLPEFHQLDGIASEDGVSLRWLLGLLAEFLERIGIREYKFRPAYFPFTEPSVE
EECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHH
AYVRVRDQWLEVLGAGLFRPEMLAALGIDYPVAAWGMGIERLAMALYDLTDIRQLYSHDV
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EFLENIPVRWWIYASSQI
HHHHCCCEEEEEEECCCC
>Mature Secondary Structure
MQGKIPVSESEHKLLELLVGRVEAGREYDTETLAELAGLDRSKVESLVRLLAEKGLVRLR
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EETMEKYVATEEAKRYLREGFPEEKLVKILAGAGGEARLDELRNAMGQELSIALANATRK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEEECCCCCC
GWVEVRGGRVRLVVDPSKAVAEERKLLEKLEIGERLQPSEFRLLRRRRLVERLAERRTIV
CCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VFPEAPEKLLEQVVVEVGALTRDLIASGRWRTVRLRRYNVAAEPPRRRLGRLNFFVEFIE
EECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
YLRDVMKEMGFQEVDDAPVELEFWNYDVLYQPQYHPARSPTDTFYLSNPSRGELPSELTP
HHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCH
RVAEAHEKGLAGSRGWRYRWSQELAARLILRSHTTAVSARVLASKPRPPFRFFTIGRVYR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEE
VETIDPRHLPEFHQLDGIASEDGVSLRWLLGLLAEFLERIGIREYKFRPAYFPFTEPSVE
EECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHH
AYVRVRDQWLEVLGAGLFRPEMLAALGIDYPVAAWGMGIERLAMALYDLTDIRQLYSHDV
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EFLENIPVRWWIYASSQI
HHHHCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]