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The map label for this gene is thiI [H]

Identifier: 124027451

GI number: 124027451

Start: 538581

End: 539747

Strand: Direct

Name: thiI [H]

Synonym: Hbut_0566

Alternate gene names: 124027451

Gene position: 538581-539747 (Clockwise)

Preceding gene: 124027450

Following gene: 124027453

Centisome position: 32.31

GC content: 54.24

Gene sequence:

>1167_bases
GTGGAGTATGAAAAGCTGGTACTTGCAGCGCTTTCCGGCGAAATAGTGTTGAAGTCAGAGCGTACAAGGCCTAGGTTTGA
GCAGAGACTAGTTGAGAATATCCATGATGCCCTTAGCCGCAATAATGTGTCGTGCAAGAGTATCGCCATAGAGTATGCAA
GGCTGCTAGTTAGGGACTGCAGCGACTACGATAAGGCGCTGGATACGCTGCTCCGAGTCTTCGGTGTCCACGGCGCAGCA
ATAGCCTACAGCTTCAAGCACAGTGGGAGCCTCGACGAGATAGCGGTAGGGGTACGAGAAATCGCGGCTGAATGGGTGAA
AGGGCGCAAGTTCGCGGTGCGCGCCAGGCGTAGTGGTGTCGAGGGCTTCACAAGCCTGGACATAGCTAGGCGTGTAGGCG
CAGAGCTCTACCCCTACTCGGCGGGCGTAGACCTAGAGAAACCCGAGGTGGAGGTATACGTTGAGGTTAGAGGACGCCAA
GTCTACGTCTACCAGGGCATGAGGCCTGGGCCTGGCGGGCTCCCCGTAGGCGTTGAGGGCCGGGCTCTCGTTCTCTTCTC
CGGCGGCCTAGACTCTCCAGTTGCGGCATGGTATACGGCTAAGAGGGGCGTCGAGGTTGACCTTCTCCACTTCATACTAG
CTGTTCCAGCATCAGCCAGGGATGCCGAGCGTGTCGGTAGGAGGCTAGCAGAACTATGGCTCTACGGGTATAAGCCAAAA
CTGTTCCTCGTAGACTTTAGGCCGGTAACAGTAACGATAGCTTCAAACACTAGGAGAGGCTATGAGCAGATAGTGCTAAG
GCTAGCAATGTACTATGCAGCACAGCTAATAGCCGAGAAGCACGGATATGACGCGGTGGTTACAGGCGAGAGTATAGGCC
AGGTTTCAAGCCAGACCCTCAAAAACATGAAAGCGATGGCCCAGGCCTTACCCCTGCGCCTCCCCCTCATAAGGCCCCTG
GCTGGCATGGATAAGGAGGAGATAGTAACGGTGTGCAGGAGGATAGGAGTCTACGAGGAAGCAGCAAAAACCAAGGAGTA
CTGCCAGATAGCGAGAGGTCTAGCAATAACTAGAGCAGACCCAAAGATTCTGAGCGAAGAGTTCGAAAAGGTAAAAGATA
TTGTTGAAGCTTCACTAGCCACGCTTAAAACTGTCGATCTCGCCTAG

Upstream 100 bases:

>100_bases
GAATGATAATAGTTGATACGTATAACATGGAGTTCATGAACGTAGAGCTAAAGCCCCGCAAGGGGTGCATGTGTCCCGAG
CCTGGGGTGTAAGCTAGGCC

Downstream 100 bases:

>100_bases
CCTCATAATACCTAGCGTGTGGATCGGAGAGTATGGGTGATGGATCAACAATAAGCTCCTCGAGCTTCTGTGCGGGTCTA
AACACGACACCCTCAACAAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MEYEKLVLAALSGEIVLKSERTRPRFEQRLVENIHDALSRNNVSCKSIAIEYARLLVRDCSDYDKALDTLLRVFGVHGAA
IAYSFKHSGSLDEIAVGVREIAAEWVKGRKFAVRARRSGVEGFTSLDIARRVGAELYPYSAGVDLEKPEVEVYVEVRGRQ
VYVYQGMRPGPGGLPVGVEGRALVLFSGGLDSPVAAWYTAKRGVEVDLLHFILAVPASARDAERVGRRLAELWLYGYKPK
LFLVDFRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIAEKHGYDAVVTGESIGQVSSQTLKNMKAMAQALPLRLPLIRPL
AGMDKEEIVTVCRRIGVYEEAAKTKEYCQIARGLAITRADPKILSEEFEKVKDIVEASLATLKTVDLA

Sequences:

>Translated_388_residues
MEYEKLVLAALSGEIVLKSERTRPRFEQRLVENIHDALSRNNVSCKSIAIEYARLLVRDCSDYDKALDTLLRVFGVHGAA
IAYSFKHSGSLDEIAVGVREIAAEWVKGRKFAVRARRSGVEGFTSLDIARRVGAELYPYSAGVDLEKPEVEVYVEVRGRQ
VYVYQGMRPGPGGLPVGVEGRALVLFSGGLDSPVAAWYTAKRGVEVDLLHFILAVPASARDAERVGRRLAELWLYGYKPK
LFLVDFRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIAEKHGYDAVVTGESIGQVSSQTLKNMKAMAQALPLRLPLIRPL
AGMDKEEIVTVCRRIGVYEEAAKTKEYCQIARGLAITRADPKILSEEFEKVKDIVEASLATLKTVDLA
>Mature_388_residues
MEYEKLVLAALSGEIVLKSERTRPRFEQRLVENIHDALSRNNVSCKSIAIEYARLLVRDCSDYDKALDTLLRVFGVHGAA
IAYSFKHSGSLDEIAVGVREIAAEWVKGRKFAVRARRSGVEGFTSLDIARRVGAELYPYSAGVDLEKPEVEVYVEVRGRQ
VYVYQGMRPGPGGLPVGVEGRALVLFSGGLDSPVAAWYTAKRGVEVDLLHFILAVPASARDAERVGRRLAELWLYGYKPK
LFLVDFRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIAEKHGYDAVVTGESIGQVSSQTLKNMKAMAQALPLRLPLIRPL
AGMDKEEIVTVCRRIGVYEEAAKTKEYCQIARGLAITRADPKILSEEFEKVKDIVEASLATLKTVDLA

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=390, Percent_Identity=29.7435897435897, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF00581 Rhodanese; PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 43038; Mature: 43038

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYEKLVLAALSGEIVLKSERTRPRFEQRLVENIHDALSRNNVSCKSIAIEYARLLVRDC
CCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
SDYDKALDTLLRVFGVHGAAIAYSFKHSGSLDEIAVGVREIAAEWVKGRKFAVRARRSGV
CHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EGFTSLDIARRVGAELYPYSAGVDLEKPEVEVYVEVRGRQVYVYQGMRPGPGGLPVGVEG
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCC
RALVLFSGGLDSPVAAWYTAKRGVEVDLLHFILAVPASARDAERVGRRLAELWLYGYKPK
CEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
LFLVDFRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIAEKHGYDAVVTGESIGQVSSQTL
EEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
KNMKAMAQALPLRLPLIRPLAGMDKEEIVTVCRRIGVYEEAAKTKEYCQIARGLAITRAD
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCC
PKILSEEFEKVKDIVEASLATLKTVDLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEYEKLVLAALSGEIVLKSERTRPRFEQRLVENIHDALSRNNVSCKSIAIEYARLLVRDC
CCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
SDYDKALDTLLRVFGVHGAAIAYSFKHSGSLDEIAVGVREIAAEWVKGRKFAVRARRSGV
CHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EGFTSLDIARRVGAELYPYSAGVDLEKPEVEVYVEVRGRQVYVYQGMRPGPGGLPVGVEG
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCC
RALVLFSGGLDSPVAAWYTAKRGVEVDLLHFILAVPASARDAERVGRRLAELWLYGYKPK
CEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
LFLVDFRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIAEKHGYDAVVTGESIGQVSSQTL
EEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
KNMKAMAQALPLRLPLIRPLAGMDKEEIVTVCRRIGVYEEAAKTKEYCQIARGLAITRAD
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCC
PKILSEEFEKVKDIVEASLATLKTVDLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA