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The map label for this gene is rpoN

Identifier: 124027419

GI number: 124027419

Start: 515018

End: 515218

Strand: Direct

Name: rpoN

Synonym: Hbut_0534

Alternate gene names: 124027419

Gene position: 515018-515218 (Clockwise)

Preceding gene: 124027418

Following gene: 124027420

Centisome position: 30.89

GC content: 50.25

Gene sequence:

>201_bases
ATGATTATTCCTATGCGTTGTTTTACGTGTGGTAGGCCTCTTGCTCAGTACTGGGAGGAGTTTCGTAGCCGCGTTGAGGG
GGGCGAAGATCCCCGGAAGGTGCTTGATGAACTTGGTGTCAAAAGGTACTGTTGTAGGAAGACCCTACTGGCTCACGTTC
CAGCCATCTATGAGGTTCGCAAGTTTAAGCGTGTACTCTAG

Upstream 100 bases:

>100_bases
CCGTTCAATGATAGCTGGTGATCCGAGACAGACGGAGCCGGAGAAGCCTATGAGGAGGAGTGCTAGGAGAAGGTGGCAGA
AGAGCTATAGGTGAAACACT

Downstream 100 bases:

>100_bases
AGGTATAACCCCCCGTCCAGCTTGGTGCGTGGAGGGGGCTAGAAGATGAGCCAGGAGTATGGGAGGCAGCAGGTAGAGAT
CGGGGGTAAGGTGGTAGAGC

Product: DNA-directed RNA polymerase subunit N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MIIPMRCFTCGRPLAQYWEEFRSRVEGGEDPRKVLDELGVKRYCCRKTLLAHVPAIYEVRKFKRVL

Sequences:

>Translated_66_residues
MIIPMRCFTCGRPLAQYWEEFRSRVEGGEDPRKVLDELGVKRYCCRKTLLAHVPAIYEVRKFKRVL
>Mature_66_residues
MIIPMRCFTCGRPLAQYWEEFRSRVEGGEDPRKVLDELGVKRYCCRKTLLAHVPAIYEVRKFKRVL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG1644

COG function: function code K; DNA-directed RNA polymerase, subunit N (RpoN/RPB10)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family

Homologues:

Organism=Homo sapiens, GI10863925, Length=62, Percent_Identity=45.1612903225806, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17509857, Length=62, Percent_Identity=43.5483870967742, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24649759, Length=62, Percent_Identity=43.5483870967742, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPON_HYPBU (A2BK83)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012739.1
- ProteinModelPortal:   A2BK83
- SMR:   A2BK83
- STRING:   A2BK83
- GeneID:   4782386
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0534
- eggNOG:   arNOG12678
- HOGENOM:   HBG391971
- OMA:   KYCCRRM
- PhylomeDB:   A2BK83
- ProtClustDB:   PRK04016
- BioCyc:   HBUT415426:HBUT_0534-MONOMER
- HAMAP:   MF_00250
- InterPro:   IPR012287
- InterPro:   IPR000268
- InterPro:   IPR020789
- Gene3D:   G3DSA:1.10.10.60
- PANTHER:   PTHR23413:SF2
- PIRSF:   PIRSF005653
- ProDom:   PD006539

Pfam domain/function: PF01194 RNA_pol_N; SSF46924 RNA_pol_N/8_sub

EC number: =2.7.7.6

Molecular weight: Translated: 7859; Mature: 7859

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01112 RNA_POL_N_8KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPMRCFTCGRPLAQYWEEFRSRVEGGEDPRKVLDELGVKRYCCRKTLLAHVPAIYEVR
CCCCCHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFKRVL
HHHHCC
>Mature Secondary Structure
MIIPMRCFTCGRPLAQYWEEFRSRVEGGEDPRKVLDELGVKRYCCRKTLLAHVPAIYEVR
CCCCCHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFKRVL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA