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The map label for this gene is trm1 [H]

Identifier: 124027351

GI number: 124027351

Start: 442841

End: 444031

Strand: Reverse

Name: trm1 [H]

Synonym: Hbut_0460

Alternate gene names: 124027351

Gene position: 444031-442841 (Counterclockwise)

Preceding gene: 124027352

Following gene: 124027347

Centisome position: 26.63

GC content: 50.97

Gene sequence:

>1191_bases
ATGGATGTAGGATTCCCAGTGAGAGTCGTACGTGAAGGCGGCGTGGAAATCGTTGTACCTGACATGGATGTCTATCGTAG
ACCCAATGGTGTCTACGAGCCTGCGTGGGCGCCAGTCTTCTATAATCCGAGGATGGCGTTCAATCGTGACATAGCGGTGG
TGTTTGCGCGTACATATGCTAGGCTACGTGGACTCGATAAGCTCGTTGTTGTAGAGCCACTTGCTGGTAGTGGTGTTAGG
GCGGTACGCTATGCTGTTGAGGCTGGGGCAATAGTGTTTGCCTCAGACATAGATTCCGACGCCGTATACCTCTCGAGAGT
TAATGCTGAGCGGAATAAGGTCTCCGAGAGAGTTCGTGTGGAAAAGGCGGATGCAAACGAGTTTATGGCGCGGCTTCCGA
GGATGGGGGTGAAGCCAACGATAATAGATATAGATCCTTTCGGTAGTCCCGCGCCATTCCTAGACACTGCTATACAAGCC
CTAAGACCCCGAGGCGTACTCGCTGTAACAGCGACAGATACAGCCCCCCTCTCTGGAACGCATCCTAGAGCTCTACGCCG
ACGCTATGATGTAAGGCCTGGAAGACTTGCATGGGAAAAAGAGCAGGCAGTACGTATACTAGCAGGCTACATTATAAGGC
GCGCAGCAGCACACGAGTATGGTGTCCGCATACTTCTAGCCTACTATGTAGACTACTATGTCCGAATCTATGCCGAGCTA
TGGCGGGGGGCAGGCCGGGCAGATAAGTCACTGGAACAGCTAGGCTACGGTGCATATTGCTACAGCTGTGGCTACACTAG
CCTAGCTAGCAATCCGTTAGAACGCTGCCCCTACTGCCAAGCACAGTTAGGCATAGTAGGCCCACTATATATCGGGAAGC
TCTGCGACGAGTCATTCATAGAGGAGATGTTGAGAGAAGCTGAGAAGATGTGGGACATACTAGCAGAACCTGATAGGGTT
GTAAAACTTCTAGAACAACTCCTCAAGGAGTGTGACATAGTAAAGCCCTATTATAGGCTCGACAAGCTATGCAGTATACT
ACGCATGAACATGCCTAAGATGAACACAGTAGTCGAGGAACTCCGTAACCGAGGCTACAATGCAACCAGAACGCATTTCG
ATCCACGCGGCTTTAAAACTAATGCACCCCACTGGGCTGTGGTAAAGATGCTCCTAGAAACTAGGCGTTAG

Upstream 100 bases:

>100_bases
CAGTGTAGGCTGTGCATCTAATGGTGTGTGGGTATATTCTTTACGGTTTCATTGTTTTTGCACCCCCACCTCTGCCAGGG
ATGTTAGGGGTATAGCTGGT

Downstream 100 bases:

>100_bases
CTCTGCATCGCCCTCCTCAACATCCTAATCTTAGTGTTGAGCCAGGCACCAAACATAGGGCAGTTTATCGGGTCGAAGAA
GCCGCCACTATGGCATTTTT

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDVGFPVRVVREGGVEIVVPDMDVYRRPNGVYEPAWAPVFYNPRMAFNRDIAVVFARTYARLRGLDKLVVVEPLAGSGVR
AVRYAVEAGAIVFASDIDSDAVYLSRVNAERNKVSERVRVEKADANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQA
LRPRGVLAVTATDTAPLSGTHPRALRRRYDVRPGRLAWEKEQAVRILAGYIIRRAAAHEYGVRILLAYYVDYYVRIYAEL
WRGAGRADKSLEQLGYGAYCYSCGYTSLASNPLERCPYCQAQLGIVGPLYIGKLCDESFIEEMLREAEKMWDILAEPDRV
VKLLEQLLKECDIVKPYYRLDKLCSILRMNMPKMNTVVEELRNRGYNATRTHFDPRGFKTNAPHWAVVKMLLETRR

Sequences:

>Translated_396_residues
MDVGFPVRVVREGGVEIVVPDMDVYRRPNGVYEPAWAPVFYNPRMAFNRDIAVVFARTYARLRGLDKLVVVEPLAGSGVR
AVRYAVEAGAIVFASDIDSDAVYLSRVNAERNKVSERVRVEKADANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQA
LRPRGVLAVTATDTAPLSGTHPRALRRRYDVRPGRLAWEKEQAVRILAGYIIRRAAAHEYGVRILLAYYVDYYVRIYAEL
WRGAGRADKSLEQLGYGAYCYSCGYTSLASNPLERCPYCQAQLGIVGPLYIGKLCDESFIEEMLREAEKMWDILAEPDRV
VKLLEQLLKECDIVKPYYRLDKLCSILRMNMPKMNTVVEELRNRGYNATRTHFDPRGFKTNAPHWAVVKMLLETRR
>Mature_396_residues
MDVGFPVRVVREGGVEIVVPDMDVYRRPNGVYEPAWAPVFYNPRMAFNRDIAVVFARTYARLRGLDKLVVVEPLAGSGVR
AVRYAVEAGAIVFASDIDSDAVYLSRVNAERNKVSERVRVEKADANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQA
LRPRGVLAVTATDTAPLSGTHPRALRRRYDVRPGRLAWEKEQAVRILAGYIIRRAAAHEYGVRILLAYYVDYYVRIYAEL
WRGAGRADKSLEQLGYGAYCYSCGYTSLASNPLERCPYCQAQLGIVGPLYIGKLCDESFIEEMLREAEKMWDILAEPDRV
VKLLEQLLKECDIVKPYYRLDKLCSILRMNMPKMNTVVEELRNRGYNATRTHFDPRGFKTNAPHWAVVKMLLETRR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [H]

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family [H]

Homologues:

Organism=Homo sapiens, GI8923219, Length=433, Percent_Identity=25.1732101616628, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI209862871, Length=433, Percent_Identity=25.1732101616628, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI216548445, Length=414, Percent_Identity=26.0869565217391, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI163792194, Length=324, Percent_Identity=25.3086419753086, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17564890, Length=406, Percent_Identity=27.5862068965517, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320325, Length=461, Percent_Identity=25.3796095444685, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19921190, Length=413, Percent_Identity=27.3607748184019, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022923
- InterPro:   IPR004039
- InterPro:   IPR002905 [H]

Pfam domain/function: PF02005 TRM [H]

EC number: =2.1.1.32 [H]

Molecular weight: Translated: 44966; Mature: 44966

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVGFPVRVVREGGVEIVVPDMDVYRRPNGVYEPAWAPVFYNPRMAFNRDIAVVFARTYA
CCCCCCCCEEECCCCEEEECCHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHH
RLRGLDKLVVVEPLAGSGVRAVRYAVEAGAIVFASDIDSDAVYLSRVNAERNKVSERVRV
HHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCHHHHHHHHHHHH
EKADANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQALRPRGVLAVTATDTAPLSGT
HHCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
HPRALRRRYDVRPGRLAWEKEQAVRILAGYIIRRAAAHEYGVRILLAYYVDYYVRIYAEL
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRGAGRADKSLEQLGYGAYCYSCGYTSLASNPLERCPYCQAQLGIVGPLYIGKLCDESFI
HHCCCCCHHHHHHHCCCCEEECCCCHHHHCCHHHHCCCCHHHHCCCCHHHHHHHCCHHHH
EEMLREAEKMWDILAEPDRVVKLLEQLLKECDIVKPYYRLDKLCSILRMNMPKMNTVVEE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LRNRGYNATRTHFDPRGFKTNAPHWAVVKMLLETRR
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDVGFPVRVVREGGVEIVVPDMDVYRRPNGVYEPAWAPVFYNPRMAFNRDIAVVFARTYA
CCCCCCCCEEECCCCEEEECCHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHH
RLRGLDKLVVVEPLAGSGVRAVRYAVEAGAIVFASDIDSDAVYLSRVNAERNKVSERVRV
HHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCHHHHHHHHHHHH
EKADANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQALRPRGVLAVTATDTAPLSGT
HHCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
HPRALRRRYDVRPGRLAWEKEQAVRILAGYIIRRAAAHEYGVRILLAYYVDYYVRIYAEL
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRGAGRADKSLEQLGYGAYCYSCGYTSLASNPLERCPYCQAQLGIVGPLYIGKLCDESFI
HHCCCCCHHHHHHHCCCCEEECCCCHHHHCCHHHHCCCCHHHHCCCCHHHHHHHCCHHHH
EEMLREAEKMWDILAEPDRVVKLLEQLLKECDIVKPYYRLDKLCSILRMNMPKMNTVVEE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LRNRGYNATRTHFDPRGFKTNAPHWAVVKMLLETRR
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA